关键词: 转基因生物（GMO）; 检测; PCR; 高通量; DSS

Abstract:

Progress in genetic engineering has led to the genetically modified organisms’(GMOs’) increasing in amount and diversification, which pose a challenge in analysis of GMO and lead to the development of analytical methods for GMO detection. The new analytical technologies unlike the traditional approaches which mostly based on the sequential detection of one target at a time, use high-throughput systems or platforms for the detection of multiple targets once. The development of the newly developing tools such as real-time PCR-based ready-to-use multi-target analytical system, padlock probe ligation in combination with microarray detection PPLMD, Luminex xMAP technology, multiplex PCR-capillary gel electrophoresis, highly sensitive bioluminometric hybridization assay and decision support systems are briefly reviewed in this paper.

Key words: genetically modified organism(GMO); detection; polymerase chain reaction (PCR); high-throughput systems; decision support systems(DSS)