关键词: 猪; 囊胚; 饲养层; 培养基; 贴壁率

Abstract: In order to improve pig parthenogenetic blastocyst adhesive rate, the experiment researchs both the culture medium and the feeder layer which can affect porcine embryo adhesive ability. Mouse, porcine and bovine fetal fibroblasts were adopted as feeder, to explore the development of the porcine parthenogenetic blastocysts cultured in DMEM or NCSU-23 or DMEM/ NCSU-23 medium. Bovine fetal fibroblasts feeder could betterly promote the porcine parthenogenetic blastocysts adherent growth, there were significant differences between the adhesion rate of bovine fetal fibroblasts feeder (33.67%) and that of mouse fetal fibroblasts feeder (19.08%)(P<0.05) , while was no significant difference between the adhesion rate of bovine fetal fibroblasts feeder and that of porcine fetal fibroblasts feeder (23.47%), the adhesion rate of mouse embryonic fibroblasts feeder group and that of the porcine fetal fibroblasts feeder groups (P>0.05). In bovine fetal fibroblasts feeder group, the blastocyst adhesion rate (22.53%) cultured in the porcine embryo culture medium NCSU-23 was significantly higher than in the DMEM culture medium (10.41%) and DMEM/NCSU-23 (12.05%) half mixed culture medium (P<0.05), and there was no significant difference between cultured in DMEM culture medium group and DMEM/NCSU-23 half mixed culture medium group (P>0.05). Bovine fetal fibroblasts feeder and porcine embryo culture medium NCSU-23 could better promote development of porcine parthenogenetic blastocyst.

Key words: porcine; embryo; feeder; culture medium; adhesive rate