›› 2010, Vol. 37 ›› Issue (6): 75-77.

• 生物技术 • 上一篇    下一篇

鹅源致病性大肠杆菌O86K61 和O44K74亚型的分离与鉴定

陈绍红1,刘艳芬2,马龙3,刘铀1   

  1. (1.广东海洋大学生化中心, 湛江 524088; 2.广东海洋大学农学院, 湛江 528088; 3.广东湛江市畜牧局, 湛江 524020)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-06-20 发布日期:2010-06-20
  • 通讯作者: 刘铀

Isolation and Identification of Pathogenic E.coli O88K61 and O44K74 Serotypes in Goos

CHEN Shao-hong1,LIU Yan-fen2,MA Long3,LIU You1   

  1. (1.Biochemistry Center of Guangdong Ocean University,Zhanjiang 524088,China; 2.Agricultural College of Guangdong 〖JP2〗Ocean University,Zhanjiang 524088,China; 3. Zhanjiang Animal Husbandry Bureau in Guangdong province,Zhanjiang 524020,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
  • Contact: LIU You

摘要: 从湛江养鹅场发生的疑似大肠杆菌病病死鹅无菌采集病料,分离鉴定出8株大肠杆菌,其中,O86K61 4株,O44K74 3株,1株未定型,上述菌株均不产生内毒素;药敏试验结果表明,大部分菌株对阿米卡星高度敏感,对恩诺沙星、先锋霉素、磺胺嘧啶、诺氟沙星、强力霉素呈交叉耐药;对万古霉素、新霉素、呋喃唑酮中度敏感;对阿莫西林、链霉素、卡那霉素、庆大霉素、四环素不敏感。上述结果提示,同一发病鹅场存在着多种致病性大肠杆菌的血清型,并存在多重耐药。

关键词: 鹅; 致病性大肠杆菌; 血清型

Abstract: Tissue samples were taken from the dead geese of breeding goose farm suspected to be colibacillosis infection,8 pathogenic E.coli strains were isolated and identified,the results of identification using O and K serogroups showed that 8 isolates were confirmed to be O88K61(4 strains) and O44K74(3 strains) serotype respectively, except 1 unidentified strain,endotoxin was not detected in the identified E.coli strains above. The antibiotics sensitivity test with 14 kinds of antibacterial drugs indicated the 8 isolates were highly sensitive to Amikacin,moderately sensitive to Vancomycin,Neomycin and Furazolidone,and resistant to Amoxinllin,Streptomycin,Kanamycin,Gentamicin and Tetracycline,the 8 E.coli isolates were cross-resistant to Enrofloxacin,Pioneermycin,Sulfadiazine,Norfloxacin and Doxycycline. The results above also revealed that at least 3 pathogenic E.coli serotypes with multi-drug resistance existed among the sick geese flock in the same farm.

Key words: goose; pathogenic E.coli; serotype

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