仔猪黄痢病原分离株药敏试验和不同给药方法疗效比较研究

›› 2010, Vol. 37 ›› Issue (2): 159-161.

仔猪黄痢病原分离株药敏试验和不同给药方法疗效比较研究

唐光武1，吴玉臣1，郭爽2，闫若潜3

(1.郑州牧业工程高等专科学校，郑州 450011； 2.河南农业大学，郑州 450002;　3.河南省动物疫病预防控制中心，郑州 450008)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-20 发布日期:2010-02-20

Study on the Drug Sensitivity Test of Pathogenic Escherichia coliIsolated from Piglet’s Yellow Dysentery and the Cure Effects of Different Drug Administration Ways

TANG Guang-wu1 , WU Yu-cen1, GUO Shuang2 , YAN Ruo-qian3

(1.Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China; 2.Henan Agricultural University, Zhengzhou 450002， China; 3.Henan Centre for Animal Disease Control and Prevention, Zhengzhou 450008, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

摘要/Abstract

摘要：

摘要：为科学评价河南省仔猪黄痢大肠杆菌地方分离株的耐药性和不同给药方法对仔猪黄痢疗效的影响，本研究首先自河南省25个临床发病猪场病猪体内分离鉴定仔猪黄痢大肠杆菌；通过小白鼠致病力试验以检测大肠杆菌分离株的毒力；采用20种抗菌药物对分离株进行药敏试验以筛选对仔猪黄痢大肠杆菌最敏感的药物；选取敏感药物分别采用口腔灌服、肌肉注射和腹腔注射3种给药方法对临床上仔猪黄痢发病猪进行治疗效果比较试验以确定最佳给药方法。结果自河南省25个发生仔猪黄痢的猪场的病猪体内共分离鉴定出25株大肠杆菌。25株大肠杆菌分离株对小白鼠的致病率为100%；对临床上常用的20种抗菌药物都有耐药现象，但耐药率高低不同（4%～100%）；20种抗菌药物中诺氟沙星、氧氟沙星、新霉素和利福平等4种药物的敏感率较高，高敏率分别为92%、88%、88%和80%。3种不同的给药方法对仔猪黄痢病例的治疗效果不同，其中通过口腔灌服和腹腔注射的给药方法对仔猪黄痢的治愈率（分别为92.3%、95.0%）显著高于肌肉注射给药方法（58.8%）；腹腔注射组治愈率稍高于口腔灌服组，但二者差异不显著。本研究为临床上仔猪黄痢的防制提供了科学依据。

关键词: 关键词：仔猪黄痢; 大肠杆菌; 药敏试验; 口腔灌服; 肌肉注射; 腹腔注射; 疗效

Abstract: Abstract：In order to scientifically evaluate the drug sensitivity of pathogenic Escherichia coli strains isolated from piglit’s yellow dysentery and the cure effects of different drug administrating ways, the pathogenic Escherichia coli(E. coli) strains were first isolated from piglets sickened yellow dysentery in 25 large-scale pig farms from Henan province. The virulence tests were carried out by analyzing pathogenicity of the isolated E. coli strains to mouse. Drug sensitive tests of the isolated bacteria strains on 20 kinds of antibiotics were carried out to estimate the most sensitive antibiotics drugs. Three different ways of drug administrating which including oral administration, intramuscular injection and intraperitoneal injection were tested to evaluate the best drug administration way. The results demonstrated that total 25 strains of E. coli were isolated from 25 sickened pig farms. All the isolated E. coli strains displayed the pathogenicity to mouse with 100% morbidity rate. Drug resistance appeared to each antibacterial drug, but the drug resistance rate was different (from 4% to 100%). Among the 20 antibacterial drugs, Norfloxacin,Floxacin,Neomycin and Rifampicin showed higher drug sensitivity to 25 E. coli isolated strains than the others, which displayed the high sensitivity rates of 92%,88%,88% and 80%, respectively .Three different ways of drug administration exhibited the different therapeutic effects on clinic sickened piglets. The drug administration ways of oral administration and intraperitoneal injection demonstrated higher cure rates (92.3% and 95.0% respectively) than that by drug administrating of intramuscular injection (58.8%）. The oral administration showed slightly higher cure rate than the intraperitoneal injection, which was no significantly statistical difference between them.

Key words: Key words：piglet’s yellow dysentery; Escherichia coli; drug sensitivity test; oral administration; intramuscular injection; intraperitoneal injection; therapeutic effect

唐光武;吴玉臣;郭爽;闫若潜. 仔猪黄痢病原分离株药敏试验和不同给药方法疗效比较研究[J]. , 2010, 37(2): 159-161.

TANG Guang-wu;WU Yu-cen;GUO Shuang;YAN Ruo-qian. Study on the Drug Sensitivity Test of Pathogenic Escherichia coliIsolated from Piglet’s Yellow Dysentery and the Cure Effects of Different Drug Administration Ways[J]. , 2010, 37(2): 159-161.

[1]	曹琪, 金清, 袁意, 冯逸雪, 孔丹妮, 陈翔鸿, 吴斌, 宋文博, 汤细彪. 规模化猪场常见呼吸道细菌性病原的流行病学调查[J]. 中国畜牧兽医, 2023, 50(6): 2439-2449.
[2]	卢玉葵, 刘佳琪, 钟嘉诚, 陈济铛, 朱婉君, 张溢珊, 张济培. 鹅源副鸡禽杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2023, 50(6): 2562-2572.
[3]	程前, 高清清, 王雨禾, 郇长超, 高崧. 禽致病性大肠杆菌外膜蛋白OmpA对DF-1细胞自噬的影响[J]. 中国畜牧兽医, 2023, 50(5): 1971-1980.
[4]	王乐平, 潘艳, 谭奕舟, 李磊, 韩凯欧, 曹雅洁, 李军, 周雨晴, 廖玉英, 马东鑫, 龚俞, 彭昊, 王晓晔. 1株大肠杆菌噬菌体vB_EcoP_GN07的分离鉴定及全基因组分析[J]. 中国畜牧兽医, 2023, 50(4): 1522-1531.
[5]	李翠函, 曲志娜, 高玉斌, 李彦, 王娟, 段笑笑, 王琳, 张喜悦, 赵格, 黄秀梅, 赵建梅, 张青青, 王君玮, 黄保续, 刘俊辉. 三类禽源碳青霉烯类耐药大肠杆菌流行病学调查及全基因组测序分析[J]. 中国畜牧兽医, 2023, 50(4): 1653-1662.
[6]	黄巧辰, 廖彬如, 余春燕, 莫玉鹏, 黄炜乾, 李磊, 韩凯欧, 王乐平, 李珣, 王晓晔. 1株大肠杆菌噬菌体EP_H11的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2023, 50(4): 1684-1694.
[7]	朱琪琪, 张继瑜, 王承业, 吴灵玉, 王玮玮, 朱阵. 河北地区动物源大肠杆菌多重耐药性与流行性分析[J]. 中国畜牧兽医, 2023, 50(3): 1081-1092.
[8]	田二杰, 谷振, 王子皓, 刘方正, 刘克森, 张雪飞, 周变华. 黄芩对安普霉素耐药大肠杆菌的耐药消除研究[J]. 中国畜牧兽医, 2023, 50(3): 1279-1286.
[9]	罗干, 邹宏, 任绍科, 吴春霞, 邹瑶, 娄银莹, 周洋, 程方俊, 郭建华. 鸭源致病性大肠杆菌O抗原与毒力基因检测及药敏试验[J]. 中国畜牧兽医, 2023, 50(2): 713-721.
[10]	王巍, 李春洙, 王沙沙, 桑锐, 葛冰洁, 李春亭, 赵欣, 刘馨蔓, 于明弘, 张雪梅. 中药联合益生菌对大肠杆菌致小鼠腹泻保护机制研究[J]. 中国畜牧兽医, 2023, 50(2): 779-788.
[11]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[12]	李佳豪, 李超, 臧长江, 孟军, 李宏坤, 贾怡琪, 麦丽克扎提·艾尔肯, 唐纳尔·托留别克. 灌胃和腹腔注射鼠李糖乳杆菌对妊娠期小鼠粪便菌群多样性的影响[J]. 中国畜牧兽医, 2023, 50(1): 151-160.
[13]	刘琳, 吴保庆, 郭晓银, 于文会, 田浪. 石榴皮水提物对猪源多重耐药大肠杆菌的抑菌作用研究[J]. 中国畜牧兽医, 2023, 50(1): 328-340.
[14]	杨福梅, 杨林富, 曾成, 杨志, 王康, 段纲, 代飞燕. 1株麂子源麦芽香肉杆菌的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2023, 50(1): 398-407.
[15]	康宇晖, 周洪善, 金文杰, 秦爱建. 1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(9): 3676-3682.