›› 2010, Vol. 37 ›› Issue (2): 156-158.

• 疾病防治 • 上一篇    下一篇

鸡传染性支气管炎病毒BJ0903株的分离鉴定

王延树1,尤永君1,2,李建丽1,杨保收1,王和平1   

  1. (1.瑞普(保定)生物技术有限公司, 保定 071000; 2.中国农业大学动物医学院, 北京 100193)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-20 发布日期:2010-02-20

Isolation and Identification on Avian Infectious Bronchitis Virus of BJ0903 Strain

WANG Yan- shu1,YOU Yong- jun 1,2,LI Jian- li1,YANG Bao- shou1,WANG He- ping1   

  1. (1.Ringpu (Baoding) Biological Pharmaceutical Co. Ltd, Baoding 071000, China; 2.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

摘要: 摘要:从北京某养鸡场疑似肾型传染性支气管炎病死鸡的肾脏和肺脏组织中分离到一株病毒,经SPF鸡胚连续传代、血凝试验、鸡胚矮小试验、干扰新城疫病毒复制试验、PCR鉴定和动物回归试验,初步确定该病毒为鸡传染性支气管炎病毒,命名为BJ0903株。

关键词: 关键词:肾型传染性支气管炎; 分离; 鉴定

Abstract:
Abstract: A strain of virus was isolated from the kidney and lung tissue of dead chicken which had similar symptoms to nephropathogentic avian infectious bronchitis on a big farm of Beijing.Contiguous passage of SPF chicken embryos,hemagglutination test,dwarfing embryonated chicken eggs,inhibition with NDV test,PCR identification and chicken recurrent infection suggested that the virus was avian infectious bronchitis.And it was named BJ0903 strain.

Key words: Key words: nephropathogentic avian infectious bronchitis; isolation; identification