›› 2010, Vol. 37 ›› Issue (11): 39-43.

• 生物技术 • 上一篇    下一篇

64株鸭致病性大肠杆菌氨基糖苷类药物耐药表型及aaC2耐药基因的调查

于学辉，陈颖，杨晓农，黄兰   

  1. （College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20

Investigation on Resistance Phenotype and Resistance Gene aaC2 against Aminoglycoside Antibiotics in 64 Strains of Pathogenic E. coli from Ducks

YU Xue-hui, CHEN Ying, YANG Xiao-nong, HUANG La   

  1. In order to investigate the existence of anti-aminoglycoside antibiotics aaC2 gene in pathogenic E. coli from ducks and to study its relationship with the resistance phenotype, the aminoglycoside antibiotics including streptomycin, neomycin, gentamycin, kanamycin, amikacin and spectinomycin were selected to conducted the drug sensitivity tests for 64 strains of E. coli from ducks by using the K-B method, and the aaC2 genes in these bacteria were detected by PCR, then the results of drug sensitivity test were compared with that of aaC2 gene detection. The results show that, of 6 kinds of drugs, the resistance rates of E. coli to streptomycin, gentamycin and amikacin are 60%, 34.5% and 4.2% respectively. The positive rate of aaC2 gene in 64 strains of isolates is 25%, the gene fragments with length of 384 bp are amplified which is uniform with the expectation, and the analysis of the sequences shows that the amplified products from 3 strains of E. coli show the homology high up to 99.7% with the corresponding sequences in GenBank. Comparison of the drug resistance results with that of resistance gene detection demonstrates that, aaC2 gene has significant influence on the resistance of E. coli to streptomycin and kanamycin, but no influence on amikacin.
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20

摘要： pathogenic E. coli from duck; aaC2 gene; aminoglycoside antibiotics; investigate

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