›› 2010, Vol. 37 ›› Issue (10): 15-17.

• 动物营养与饲料科学 • 上一篇    下一篇

天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响

康乐, 姚东璧, 胡庭俊, 李晓栩   

  1. (广西大学动物科学技术学院, 南宁 530005)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 胡庭俊

The Effect of Asparagus Cochinchinensis Polysaccharide on Proliferation of Splenic Lymphocytes from Mice in vitro

KANG Le, YAO Dong-bi,HU Ting-jun,LI Xiao-xu   

  1. (College of Animal Science and Technology, Guangxi University, Nanning 530005, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: HU Ting-jun

摘要: 本试验旨在研究天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响。从正常小鼠分离脾脏淋巴细胞体外培养,在细胞培养体系中分别加入终浓度为50、100、200、400 mg/L天门冬多糖或配合刀豆素(5 mg/L)作用24、48、72 h,以MTT法测定细胞的增殖程度。结果表明,天门冬多糖可显著促进体外培养的小鼠脾脏淋巴细胞增殖,并与ConA有协同作用。

关键词: 天门冬; 多糖; 小鼠; 淋巴细胞; 增殖

Abstract: To investigate immunomodulatory effect of Asparagus cochinchinensis polysaccharide (ACP),the murine splenic lymphocytes cells were incubated in RPMI-1640 tissue culture medium supplemented with ConA (final concentration 5 mg/L ). ACP was added at concentrations of 0, 50, 100, 200 or 400 mg /L on 24,48,72 h, respectively. The cell proliferation were observed. The results showed that ACP was able to stimulate proliferation of splenic lymphocytes within 50 to 400 mg/L.

Key words: Asparagus cochinchinensis; polysaccharide; mice; lymphocytes; proliferation

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