›› 2009, Vol. 36 ›› Issue (12): 31-35.

• 生理生化 • 上一篇    下一篇

rmtB阳性猪大肠埃希氏菌中氨基糖苷钝化酶分析

陈琳1,2，张俊丰3，刘健华1，陈杖榴1，曾振灵1   

  1. （1.华南农业大学兽医学院， 广东省兽药研制与安全评价重点实验室， 广州 510642； 2.江苏畜牧兽医职业技术学院， 泰州 225300； 3.重庆市畜牧科学院， 荣昌 402460）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-12-20 发布日期:2009-12-20

Characterization of Aminoglycosides Modifying Enzymes in rmtB-positive Escherichia coli

CHEN Lin1,2, ZHANG Jun-feng3, LIU Jian-hua1, CHEN Zhang-liu1, ZENG Zhen-ling1   

  1. (1.Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.Jiangsu Animal Science and Veterinary Medicine College, Taizhou 225300, China; 3.Chongqing Academy of Animal Science, Rongchang 402460, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20

摘要： 分析rmtB阳性猪大肠埃希氏菌中氨基糖苷钝化酶的流行特点。设计10对引物，采用PCR、RFLP和测序等方法检测分析rmtB阳性猪大肠埃希氏菌中的10种氨基糖苷钝化酶基因。采用微量稀释法测定rmtB阳性猪大肠埃希氏菌及其接合子对10种氨基糖苷类的敏感性。48株rmtB阳性猪大肠埃希氏菌中检测到7种氨基糖苷类钝化酶基因，检出率从高到低依次是aac(3)-II(85.8%)、aph(3′)-VII(77.6%)、aph(3′)-II(77.6%)、aadA1(34.7%)、aac(6′)-Ib(14.3%)、aac(3)-IV(10.2%)和aph(4)-I(8.2%)；48株rmtB阳性猪大肠埃希氏菌对卡那霉素、阿米卡星、庆大霉素、妥布霉素、西索米星、萘替米星、新霉素、链霉素、大观霉素及安普霉素的耐药率分别为100%、100%、100%、100%、100%、100%、79.2%、77.1%、89.6%和27.1%。其对氨基糖苷类抗菌药的耐药表型与钝化酶基因型的符合率最高约98%。研究结果表明，氨基糖苷钝化酶广泛存在于rmtB阳性猪大肠埃希氏菌中，它们与其他氨基糖苷类耐药机制共同介导受试菌对氨基糖苷类抗菌药的耐药性。

关键词: 大肠埃希氏菌; 氨基糖苷钝化酶; 耐药; RmtB

Abstract: To analyse the prevalent characterization of aminoglycosides modifying enzymes in rmtB-positive Escherichia coli from pig farms. The polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and DNA sequencing were used to detect and identify the resistant genes encoding aminoglycoside modifying enzymes. Micro-dilution method was adopted to evaluate the MICs to 10 aminoglycosides. Total of 7 aminoglycoside modifying enzymes were presented in 48 rmtB-positive Escherichia coli from pig farms.And the present ratio of aac(3)-II, aph(3′)-II, aph(3′)-VII, aadA1, aac(6′)-Ib, aac(3)-IV and aph(4)-I were 85.8%, 77.6%, 77.6%, 34.7%, 14.3%, 10.2% and 8.2% by turn.The resistant ratio of the 48 rmtB-positive Escherichia coli to Kanamycin, Amikacin, Gentamycin, Tobramycin, Sisomicin, Netilmicin, Neomycin, Streptomycin, Spectinomycin and Apramycin were 100%, 100%, 100%, 100%, 100%, 100%, 79.2%, 77.1%, 89.6% and 27.1% respectively. The highest coincidence was 98% between the phenotypes to aminoglycosides and genotype of AMEs. It indicated that aminoglycoside modifying enzymes were prevalent in rmtB-positive Escherichia coli. They mediated the resistance of Escherichia coli to aminoglycosides with 16S rRNA methylase-RmtB.

Key words: Escherichia coli; aminoglycosides modifying enzymes (AMEs); resistance; RmtB

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