国标法对乳碱性磷酸酶的检测及其在巴氏消毒上的研究

›› 2007, Vol. 34 ›› Issue (9): 68-70.

国标法对乳碱性磷酸酶的检测及其在巴氏消毒上的研究

惠永华，杨国宇，韩立强，王伟杰，苗培，林茂旺

河南农业大学牧医工程学院，郑州 450002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-20 发布日期:2007-09-20

The Study on the Determination of Alkaline Phoaphate by the National Standard in Milk and the Application in the Pasteurization

HUI Yonghua,YANG Guoyu,HAN Liqiang,WANG Weijie,MIAO Pei,LIN Maowang

The College of Animal Husbandry and Veterimary in Henan Agriculture University,Zhengzhou 450002,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

摘要/Abstract

摘要： 采用国标法对乳中碱性磷酸酶（ALP）检测，结果显示：全乳和脱脂乳的酶活力分别为403.345、148.8147 μg/ml，变异系数分别为1.30%、2.99%。与国标法的不同之处：乳样最佳稀释比为1∶25，苯酚的最佳浓度为50 μg/ml；然后对其模拟巴氏消毒得出：63℃ 30 min基本使ALP灭活。

关键词: 国标法; 碱性磷酸酶; 巴氏消毒

Abstract: The result of using the national standard to measure the alkaline phosphate (ALP)in milk is as follows: the activity of enzyme in the whole milk and skimmed milk is 403.345 μg/ml, 148.8147 μg/ml respectively;the coefficient of variation is 1.30%，2.99%， respectively.All these point this method is good and stable.The difference compounded to the national standard: the best dillution ratio of milk samples is 1∶25, the right consistency of phenol is 50 μg/ml. The conclusion by simulating pasteurization: ALP is inactived at 63℃ for 30 min.

Key words: national standard; alkaline phosphate; pasteurization

中图分类号:

S879.1

惠永华;杨国宇;韩立强;王伟杰;苗培;林茂旺 . 国标法对乳碱性磷酸酶的检测及其在巴氏消毒上的研究[J]. , 2007, 34(9): 68-70.

HUI Yonghua;YANG Guoyu;HAN Liqiang;WANG Weijie;MIAO Pei;LIN Maowang. The Study on the Determination of Alkaline Phoaphate by the National Standard in Milk and the Application in the Pasteurization[J]. , 2007, 34(9): 68-70.

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