《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (6): 1890-1894.doi: 10.16431/j.cnki.1671-7236.2017.06.044

• 基础兽医 • 上一篇    

黄芩苷对大肠杆菌细胞通透性的影响

刘昊, 赵自冰, 吴丹丹, 王新   

  1. 黑龙江八一农垦大学动物科技学院, 大庆 163319
  • 收稿日期:2016-12-28 出版日期:2017-06-20 发布日期:2017-06-28
  • 通讯作者: 王新 E-mail:wangxin777@byau.edu.cn
  • 作者简介:刘昊(1992-),男,辽宁盘锦人,硕士生,研究方向:兽医药理与毒理学,E-mail:2352225285@qq.com
  • 基金资助:

    黑龙江省教育厅项目(12531473)

Effect of Baicalin on Cell Permeability of Escherichia coli

LIU Hao, ZHAO Zi-bing, WU Dan-dan, WANG Xin   

  1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2016-12-28 Online:2017-06-20 Published:2017-06-28

摘要:

本试验旨在探索黄芩苷对大肠杆菌的抑菌机制。在测定黄芩苷对大肠杆菌的最小抑菌浓度和生长曲线的基础上,通过测定菌液电导率和碱性磷酸酶含量,研究黄芩苷对大肠杆菌细胞通透性的影响。试验结果表明,黄芩苷对大肠杆菌具有明显的抑菌效果,其最小抑菌浓度为6.25 mg/mL;黄芩苷可引起大肠杆菌菌液的电导率和碱性磷酸酶含量明显升高。证实了黄芩苷通过增加大肠杆菌细胞膜与细胞壁通透性,从而发挥抑菌作用。

关键词: 黄芩苷; 大肠杆菌; 电导率; 碱性磷酸酶

Abstract:

The objective of this study was to explore the antibacterial mechanism of baicalin on Escherichia coli. Based on the determination of baicalin on Escherichia coli minimum inhibitory concentration (MIC) and growth curve, the influence of baicalin on Escherichia coli cells permeability was researched by measuring the conductivity and alkaline phosphatase (AKP) content of bacterial solution. The experimental results showed that baicalin had significantly inhibitory effect on Escherichia coli, and its MIC was 6.25 mg/mL.Baicalin could significantly increase the conductivity and AKP content of Escherichia coli bacteria solution.The results suggested that baicalin on antibacterial activity was mainly due to the changes of the cell membrane permeability and the damage of the cell wall.

Key words: baicalin; Escherichia coli; conductivity; alkaline phosphatase

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