雪白睡莲花粗多糖提取工艺优化及其对金黄色葡萄球菌性小鼠乳腺炎的治疗效果研究

doi:10.16431/j.cnki.1671-7236.2025.06.034

摘要/Abstract

摘要： 【目的】优化雪白睡莲花粗多糖的提取工艺，并探究其在金黄色葡萄球菌感染小鼠乳腺炎治疗中的应用效果。【方法】以雪白睡莲花为试验材料，以雪白睡莲花粗多糖的提取率为评价指标，采用单因素结合响应面法优化其提取工艺。将60只SPF级健康昆明小鼠随机分为4组：空白对照组(BC)、模型对照组(MC)、阳性对照组(PC)、雪白睡莲花粗多糖组(CPNA)，每组15只，除空白对照组外其余各组小鼠注射50 μL 1×10⁴ CFU/mL金黄色葡萄球菌构建小鼠乳腺炎模型,造模成功后各组小鼠分别灌胃生理盐水、0.13 g/kg环丙沙星、0.5 g/kg雪白睡莲花粗多糖,每天2次,连续给药7 d,空白对照组小鼠不做处理。分别在给药后3、5、7 d测定各试验组小鼠乳腺组织中金黄色葡萄球菌菌落数量、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量，同时观察小鼠乳腺病理组织学变化，从而评估其治疗效果。【结果】在料液比为1∶14，超声功率为70%，提取温度为57 ℃，提取时间为30 min，提取次数为2次的条件下，雪白睡莲花粗多糖得率为51.78%。给药后第5、7天，雪白睡莲花粗多糖组小鼠乳腺组织菌落数量、TNF-α、IL-6含量均显著或极显著低于模型对照组(P＜0.05；P＜0.01)，乳腺组织中炎症细胞和红细胞明显减少。【结论】使用单因素结合响应面法优化提取方法后，雪白睡莲花粗多糖得率达51.78%，其对金黄色葡萄球菌性小鼠乳腺炎动物模型具有良好的治疗效果。本研究结果可为临床乳腺炎的防治及雪白睡莲花的开发利用提供科学依据。

关键词: 雪白睡莲花; 粗多糖; 提取工艺; 金黄色葡萄球菌; 乳腺炎

Abstract: 【Objective】 The aim of the experiment was to optimize the extraction process of crude polysaccharide from Nymphaea candida and explore its application effect in the treatment of mastitis in mice infected with Staphylococcus aureus.【Method】 The experiment used Nymphaea candida as the test material,and the extraction rate of Nymphaea candida crude polysaccharide as the evaluation index,and the one-way factor combined with response surface method was used to optimize its extraction process.At the same time,60 SPF healthy Kunming mice were randomly divided into four groups:Blank control group (BC),model control group (MC),positive control group (PC),and crude polysaccharides from Nymphaea candida (CPNA) group,15 mice in each group.50 μL Staphylococcus aureus with 1×10⁴ CFU/mL was injected into mice in each group except for blank control group to construct a mouse mastitis model.After successful modelling,saline,0.13 g/kg ciprofloxacin,and 0.5 g/kg crude polysaccharides from Nymphaea candida were administered by gavage to mice in each group twice a day for 7 d,respectively.Mice in blank control group were not treated.The number of Staphylococcus aureus colonies,the content of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mammary tissues of mice in each test group were measured at 3,5 and 7 d of administration,respectively,and the pathological histological changes in mammary glands of the mice were also observed to evaluate the therapeutic effect.【Result】 Under the conditions of material-liquid ratio was 1∶14,ultrasonic power was 70%,extraction temperature was 57 ℃,extraction time was 30 min,and the number of extraction times was 2,the yield of Nymphaea candida crude polysaccharide was 51.78%.On the 5th and 7th days,the number of colonies in mammary tissue,TNF-α and IL-6 contents of mice in CPNA group showed significantly or extremely significantly lower than that in model control group (P＜0.05 or P＜0.01),and the inflammatory cells and erythrocytes in mammary tissues of mice were significantly reduced.【Conclusion】 After optimizing the extraction method using one-factor combined response surface method,the yield of crude polysaccharide from Nymphaea candida was 51.78%.It had a good therapeutic effect on the animal model of mouse mastitis induced by Staphylococcus aureus.The results of this study could provide a scientific basis for the prevention and treatment of clinical mastitis and the development and utilization of Nymphaea candida.

Key words: Nymphaea candida; crude polysaccharide; extraction process; Staphylococcus aureus; mastitis

中图分类号:

S853.7

刘丹丹, 罗洁, 高函雅, 徐李依娜, 郝宝山, 王馨, 张伟. 雪白睡莲花粗多糖提取工艺优化及其对金黄色葡萄球菌性小鼠乳腺炎的治疗效果研究[J]. 中国畜牧兽医, 2025, 52(6): 2818-2829.

LIU Dandan, LUO jie, GAO Hanya, XU Liyina, HAO Baoshan, WANG Xin, ZHANG Wei. Optimization of the Extraction Process of Crude Polysaccharides from Nymphaea candida and Its Therapeutic Effect on Mouse Mastitis Induced by Staphylococcus aureus[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2818-2829.

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