N-氨基甲酰谷氨酸对绒山羊胎盘发育影响的研究

doi:10.16431/j.cnki.1671-7236.2025.06.021

摘要/Abstract

摘要： 【目的】探究饲粮中添加N-氨基甲酰谷氨酸(N-carbamylglutamate,NCG)对母羊胎盘形态、羔羊初生窝重及体尺、血浆抗氧化指标以及血液和胎盘中血管内皮生长因子(vascular endothelial growth factor，VEGF)的影响，以了解NCG在绒山羊胎盘发育中的作用。【方法】试验选择60只8月龄、体重(45.0±2.3) kg、体况良好的辽宁绒山羊母羊，经同期发情后人工授精，利用B超检查技术记录同期受孕的母羊，随机分为对照组(CON，全混合饲粮)和 NCG组(含0.09% NCG的全混合饲粮)。试验预试期1周，正试期22周。试验第18周结束时对怀孕母羊进行颈静脉采血，通过试剂盒测定血浆抗氧化指标；妊娠期结束后采集胎盘，统计胎盘重量、胎盘绒毛叶的重量和数量，计算绒毛叶的均度与密度，记录羔羊的初生窝重和初生体重，测量羔羊的体高、体长和胸围。【结果】与CON组相比，NCG组绒山羊母羊胎盘的重量和羔羊初生窝重、初生体重和胸围均显著升高(P＜0.05)；NCG组母羊血浆中一氧化氮(NO)、VEGF和血管内皮生长因子A(VEGFA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性均显著升高(P＜0.05)。NCG组母羊胎盘中的VEGF和VEGFA含量均显著低于CON组母羊(P＜0.05)。【结论】NCG可通过NO路径影响妊娠期绒山羊机体抗氧化能力、提高机体VEGF水平，进而促进胎盘和胎儿发育。研究结果为NCG在绒山羊母羊饲粮中的应用提供理论依据。

关键词: 绒山羊; N-氨基甲酰谷氨酸(NGG); 胎盘; 血管内皮生长因子(VEGF)

Abstract: 【Objective】 The objective of this study was to investigate the effects of dietary supplementation of N-carbamyl glutamate (NCG) on placental morphology,newborn litter weight and body size of lambs,plasma antioxidant indexes,and vascular endothelial growth factor (VEGF) in blood and placenta，in order to understand the role of NCG in placental development of cashmere goats.【Method】 60 ewe of Liaoning cashmere goat,weighing (45.0±2.3) kg and in good condition,aged 8 months,were selected,and artificially inseminated after estrus at the same time,and the ewes conceived at the same time were recorded by B-ultrasound examination technology.They were randomly divided into control group (CON，total mixed diet) and NCG group (total mixed diet containing 0.09% NCG).The pre-trial period was 1 week and the formal period was 22 weeks.At the end of the 18th week of the experiment,blood samples were collected from the jugular vein of pregnant ewes,and plasma antioxidant indexes were determined by the kit.The placenta was collected after the end of pregnancy,the weight of placenta,the weight and number of villous leaves were calculated,the uniformity and density of villous leaves were calculated,the newborn litter weight and newborn weight of lambs were recorded,and the body height,body length and chest circumference of lambs were measured.【Result】 Compared with CON group,the placenta weight,newborn litter weight and newborn weight and chest circumference of lambs in NCG group were significantly increased (P＜0.05).The contents of nitric oxide (NO),VEGF and vascular endothelial growth factor A (VEGFA) and the activity of glutathione peroxidase (GSH-Px) in plasma of ewe in NCG group were significantly increased (P＜0.05).The contents of VEGF and VEGFA in placenta of ewe in NCG group were significantly lower than those in CON group (P＜0.05).【Conclusion】 NCG could affect the antioxidant capacity of pregnant cashmere goats and increase the level of VEGF through NO pathway,thus promoting the development of placenta and fetus.The results provided a theoretical basis for the application of NCG in the diet of cashmere goat ewes.

Key words: cashmere goats; N-carbamylglutamate (NCG); placenta; vascular endothelial growth factor (VEGF)

中图分类号:

S827.5

栗铭谦, 常帅, 马桂霞, 韩迪, 全治国, 豆兴堂, 马巍, 王春强. N-氨基甲酰谷氨酸对绒山羊胎盘发育影响的研究[J]. 中国畜牧兽医, 2025, 52(6): 2684-2690.

LI Mingqian, CHANG Shuai, MA Guixia, HAN Di, QUAN Zhiguo, DOU Xingtang, MA Wei, WANG Chunqiang. Study on the Effect of N-carbamoylglutamate on Placental Development in Cashmere Goats[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2684-2690.

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