新疆喀什某规模化安格斯牛场犊牛产气荚膜梭菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2024.05.039

中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (5): 2188-2197.doi: 10.16431/j.cnki.1671-7236.2024.05.039

新疆喀什某规模化安格斯牛场犊牛产气荚膜梭菌的分离鉴定及耐药性分析

马紫薇¹, 马学军², 马亚楠³, 孙亚伟¹, 吾买尔江·牙合甫¹, 邵白新月¹, 杨作斌¹, 王选艺¹, 王梦娇¹, 钟旗⁴, 马雪连¹, 姚刚¹

1. 新疆农业大学动物医学学院, 乌鲁木齐 830000;
2. 新疆昌吉市二六工镇农业(畜牧)发展服务中心, 昌吉 831100;
3. 新疆维吾尔自治区昌吉回族自治州农产品质量安全中心, 昌吉 831100;
4. 新疆畜牧科学院兽医研究所, 乌鲁木齐 830000

收稿日期:2023-10-09 出版日期:2024-05-05 发布日期:2024-04-28
通讯作者: 马雪连, 姚刚 E-mail:maxuelian@xjau.edu.cn;yg@xjau.edu.cn
作者简介:马紫薇,E-mail:2027214787@qq.com。
基金资助:
自治区乡村振兴项目(2023NC023);自治区天山创新团队项目(2023D14018)

Isolation,Identification and Drug Resistance Analysis of Clostridium perfringens in Calves in a Large-scale Angus Beef Cattle Farm in Kashgar,Xinjiang

MA Ziwei¹, MA Xuejun², MA Yanan³, SUN Yawei¹, OMAR Yakup¹, SHAOBAI Xinyue¹, YANG Zuobin¹, WANG Xuanyi¹, WANG Mengjiao¹, ZHONG Qi⁴, MA Xuelian¹, YAO Gang¹

1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830000, China;
2. Agricultural(Animal Husbandry) Development Service Center, Erliugong Town, Changji City, Changji 831100, China;
3. Agricultural Products Quality and Safety Center, Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Region, Changji 831100, China;
4. Institute of Veterinary Medicine, Xinjiang Academy of Animal Sciences, Urumqi 830000, China

Received:2023-10-09 Online:2024-05-05 Published:2024-04-28

摘要/Abstract

摘要： 【目的】产气荚膜梭菌(Clostridium perfringens,CP)是一种革兰阳性厌氧菌,可导致犊牛猝死、出血性坏死性肠炎、肠毒血症等,对犊牛健康造成巨大影响。本研究旨在了解新疆喀什地区某规模化肉牛场安格斯犊牛产气荚膜梭菌流行现状及耐药情况,为该地区由产气荚膜梭菌引起的疾病的治疗提供科学依据。【方法】试验采集20份组织样品及6份圈舍粪土样品,采用分离培养、形态学观察、染色镜检、生化试验等方法进行细菌分离鉴定,并采用K-B纸片法进行药敏试验,PCR检测分离菌的毒力型及耐药基因。【结果】分离菌在FTG液体培养基中出现浑浊并产生大量气体,在TSC培养基中长出黑色边缘整齐的圆形菌落,在绵羊血平皿上长出具有典型的双溶血环菌落,革兰染色镜检可见菌体粗短、成单个或双个排列的革兰阳性直杆菌,形态学及镜检结果符合产气荚膜梭菌特点。26份样品中共分离出10株产气荚膜梭菌,检出率为38.46%。经毒素基因分型鉴定,其中,A型产气荚膜梭菌9株,检出率为90.0%,D型产气荚膜梭菌1株,检出率为10.0%。药敏试验结果显示,分离株对卡那霉素耐药率最高,为80.0%;对庆大霉素和林可霉素耐药率为50.0%,而其对美罗培南、头孢吡肟、氟苯尼考及多西环素敏感。耐药基因检测结果表明,bla_TEM基因检出率最高,为100.0%;bla_SHV、qnrA和aac(6')-Ⅰb-cr基因检出率分别为20.0%(2/10)、10.0%(1/10)和20.0%(2/10);未检出bla_CTX-M和qnrS 2种耐药基因。【结论】新疆喀什地区安格斯犊牛产气荚膜梭菌感染主要以A型为主,耐药情况严重,且存在多重耐药。试验结果可为后期产气荚膜梭菌流行病学研究和防控提供科学依据。

关键词: 安格斯犊牛; 产气荚膜梭菌; 分离鉴定; 耐药性

Abstract: 【Objective】 Clostridium perfringens (CP) is a Gram-positive anaerobic bacterium that can cause sudden calf death,hemorrhagic necrotizing enteritis,enterotoxemia,etc.,which has a huge impact on calf health. The experiment was aimed to understand the prevalence and drug resistance of Clostridium perfringens in Angus calves in a large-scale beef cattle farm in Kashgar prefecture,Xinjiang,so as to provide a scientific basis for the treatment of diseases caused by Clostridium perfringens in this region.【Method】 Twenty tissue samples and six enclosure manure samples were collected for isolation and identification of bacteria by separation and culture,morphological observation,staining microscopy,biochemical test,etc., the drug susceptibility test was carried out by K-B paper method,and the virulence type and drug resistance genes were detected by PCR.【Result】 The isolates appeared turbid in FTG liquid medium and produced a large amount of gas,round colonies with neat black edges grew in TSC medium,and colonies with typical double hemolytic rings grew on sheep blood plates.Microscopic examination of Gram staining showed that the bacteria were coarse and short,in single or double arrangement,and the morphology and microscopic examination results were consistent with the characteristics of Clostridium perfringens.A total of 10 strains of Clostridium perfringens were isolated in 26 samples,with a detection rate of 38.46%.After toxin genotyping,9 strains of Clostridium perfringens type A were identified with a detection rate of 90.0%,and 1 strain of Clostridium perfringens type D was identified with a detection rate of 10.0%.Susceptibility test results showed that the isolates had the highest resistance rate of 80.0% to kanamycin,50.0% to gentamicin and lincomycin,and were sensitive to meropenem,cefepime,florfenicol and doxycycline.The results of drug resistance genes showed that the detection rate of bla_TEM gene was the highest,which was 100.0%,and the detection rates of bla_SHV,qnrA and aac(6')-Ⅰb-cr resistance genes were 20.0% (2/10),10.0% (1/10) and 20.0% (2/10),respectively,and the two resistance genes of bla_CTX-M and qnrS were not detected.【Conclusion】 Clostridium perfringens infection in Angus calves in Kashgar prefecture,Xinjiang was mainly type A,with serious drug resistance and multiple drug resistance.The results of this study could provide a scientific basis for epidemiological research and prevention and control of Clostridium perfringens in the later stage.

Key words: Angus calves; Clostridium perfringens; isolation and identification; drug resistance

中图分类号:

S852.61^＋6.3

马紫薇, 马学军, 马亚楠, 孙亚伟, 吾买尔江·牙合甫, 邵白新月, 杨作斌, 王选艺, 王梦娇, 钟旗, 马雪连, 姚刚. 新疆喀什某规模化安格斯牛场犊牛产气荚膜梭菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2024, 51(5): 2188-2197.

MA Ziwei, MA Xuejun, MA Yanan, SUN Yawei, OMAR Yakup, SHAOBAI Xinyue, YANG Zuobin, WANG Xuanyi, WANG Mengjiao, ZHONG Qi, MA Xuelian, YAO Gang. Isolation,Identification and Drug Resistance Analysis of Clostridium perfringens in Calves in a Large-scale Angus Beef Cattle Farm in Kashgar,Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2188-2197.

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新疆喀什某规模化安格斯牛场犊牛产气荚膜梭菌的分离鉴定及耐药性分析

Isolation,Identification and Drug Resistance Analysis of Clostridium perfringens in Calves in a Large-scale Angus Beef Cattle Farm in Kashgar,Xinjiang

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