二丁提取散的抗炎、镇痛和解热作用研究

doi:10.16431/j.cnki.1671-7236.2023.09.042

摘要/Abstract

摘要： 【目的】研究二丁提取散的抗炎、镇痛和解热作用。【方法】利用二甲苯致小鼠耳廓肿胀、冰醋酸致小鼠扭体、脂多糖(LPS)致家兔发热法来研究二丁提取散的抗炎、镇痛和解热作用。试验小鼠随机分为5组,每组10只,分别为二丁提取散低、中、高剂量组,阳性对照组(蒲地蓝组/阿司匹林组)和模型对照组。二丁提取散低、中、高剂量组及蒲地蓝组每日分别以含生药3.9、7.8、15.6和4.8 g/kg的剂量灌胃给药1次,连续给药4 d,阿司匹林组于第4天灌胃0.2 g/kg阿司匹林,模型对照组灌服等量纯化水,给药结束后按照不同试验具体操作。家兔经耳缘静脉注射0.3 mL/kg LPS溶液(2 μg/mL)构建LPS致热模型,将满足试验要求的家兔随机分为5组,其中二丁提取散低、中、高剂量组以及阿司匹林组分别以含生药1.4、2.8、5.6和0.1 g/kg剂量灌胃给药,模型对照组灌服等量纯化水,分别于给药后1、2、3、4、5、6 h测量家兔体温变化情况。【结果】二甲苯致小鼠耳廓肿胀试验结果显示,与模型对照组相比,二丁提取散高、中剂量组小鼠耳廓肿胀程度极显著或显著降低(P＜0.01;P＜0.05);与蒲地蓝组相比,二丁提取散各剂量组差异不显著(P＞0.05)。冰醋酸致小鼠扭体试验结果显示,与模型对照组相比,二丁提取散高、中剂量组小鼠扭体数极显著或显著减少(P＜0.01;P＜0.05);与阿司匹林组相比,二丁提取散高剂量组差异不显著(P＞0.05),中、低剂量组小鼠扭体数显著或极显著增加(P＜0.05;P＜0.01)。LPS致家兔发热试验结果显示,与模型对照组相比,给药后2 h,阿司匹林组及二丁提取散高、中剂量组家兔体温极显著或显著降低(P＜0.01;P＜0.05);与阿司匹林组相比,给药后2 h,二丁提取散中剂量组家兔体温显著升高(P＜0.05)。【结论】二丁提取散具有一定的抗炎、镇痛和解热作用,研究结果为二丁提取散的临床应用及进一步研究提供理论依据。

关键词: 二丁提取散; 抗炎; 镇痛; 解热

Abstract: 【Objective】 The purpose of this experiment was to study the anti-inflammatory,analgesic and antipyretic effects of Erding extract powder.【Method】 The anti-inflammatory,analgesic and antipyretic effects of Erding extract powder were studied by xylene induced auricle swelling,glacial acetic acid induced body twisting in mice and lipopolysaccharide (LPS) induced fever in rabbits.The experimental mice were randomly divided into 5 groups,10 mice in each group,which were low,medium and high dose groups of Erding extract powder,positive control group (Pudilan group/aspirin group) and model control group.Erding extract powder low,medium and high dose groups and Pudilan group were given intragastric administration of 3.9,7.8,15.6 and 4.8 g/kg crude drug,respectively,once a day for 4 consecutive days.Aspirin group was given intragastric administration of 0.2 g/kg aspirin on the fourth day,and model control group was given an equal amount of purified water.After administration,specific operations were performed according to different experiments.Rabbits were injected with 0.3 mL/kg LPS solution (2 μg/mL) through ear vein to establish LPS thermogenic model,which were randomly divided into 5 groups,including low,medium and high dose groups of Erding extract powder,and aspirin group were administrated intragastric at doses containing crude drug 1.4,2.8,5.6 and 0.1 g/kg,respectively.The model control group was given an equal amount of purified water,and the changes of rabbit body temperature were measured at 1,2,3,4,5 and 6 h after administration.【Result】 The results of auricle swelling induced by xylene in mice showed that,compared with model control group,auricle swelling of mice in high and medium dose groups of Erding extract powder were extremely significantly or significantly decreased (P<0.01 or P<0.05).Compared with Pudilan group,there was no significant difference in each dose group of Erding extract powder (P>0.05).The writhing test of mice induced by glacial acetic acid showed that compared with model control group,the body writhing of mice in high and medium dose groups of Erding extract powder were extremely significantly or significantly decreased (P<0.01 or P<0.05).Compared with aspirin group,there was no significant difference of the body writhing of mice in Erding extract powder high dose group (P>0.05),but significant or extremely significant difference between medium and low dose groups (P<0.05 or P<0.01).The results of LPS fever test showed that compared with model control group,2 h after administration,the body temperature of rabbits in aspirin group and Erding extract powder high and medium dose groups were extremely significantly or significantly decreased (P<0.01 or P<0.05).Compared with aspirin group,2 h after administration, the body temperature of rabbits in Erding extract powder medium dose group was significant increased (P ＜0.05).【Conclusion】 The results showed that Erding extract powder had good anti-inflammatory,analgesic and antipyretic effects,which provide theoretical basis for clinical application and further research of Erding extract powder.

Key words: Erding extract powder; anti-inflammatory; analgesic; antipyretic

中图分类号:

S853.7

陈昱骁, 付海宁, 陈玲, 栾明娜, 张会梅, 姚德勇, 王艳玲, 蔡青秀, 章安源, 尹伶灵, 孙亚磊. 二丁提取散的抗炎、镇痛和解热作用研究[J]. 中国畜牧兽医, 2023, 50(9): 3861-3868.

CHEN Yuxiao, FU Haining, CHEN Ling, LUAN Mingna, ZHANG Huimei, YAO Deyong, WANG Yanling, CAI Qingxiu, ZHANG Anyuan, YIN Lingling, SUN Yalei. Study on the Anti-inflammatory,Analgesic and Antipyretic Effects of Erding Extract Powder[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3861-3868.

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