致病性大肠杆菌HPI毒力岛及其水平转移的研究进展

doi:10.16431/j.cnki.1671-7236.2023.08.041

摘要/Abstract

摘要： 致病性大肠杆菌作为肠杆菌科成员和人兽共患病病原体之一,在一定条件下可通过多种途径引起人和动物感染并引发不同的大肠杆菌病病型。由于致病性大肠杆菌的血清型和毒力因子众多、耐药趋势严峻、存在生物被膜等因素导致大肠杆菌病的流行趋势复杂且疫苗研发困难,给国内外的畜禽养殖业带来一定的经济损失。高致病性岛(high-pathogenicity island,HPI)最早发现于耶尔森菌,被证实是其毒力表型所必需的基因组岛,为强毒力岛。由于细菌的水平转移机制让该毒力岛在其他肠杆菌科中被广泛检出。其中HPI毒力岛也在致病性大肠杆菌如肠出血性大肠杆菌(enterohemorrhage Escherichia coli,EHEC)、产志贺毒素大肠杆菌(shiga toxin-producing Escherichia coli,STEC)等中被大量检出,被证实影响致病性大肠杆菌毒力,参与致病性大肠杆菌铁元素摄取和介导宿主免疫反应等。为进一步了解HPI毒力岛在致病性大肠杆菌中的铁元素调控机理、致病特性、介导的细胞免疫反应和水平转移途径,现从HPI毒力岛结构、如何调控耶尔森菌素铁载体、HPI毒力岛致病性及调控细胞免疫反应、HPI毒力岛的水平转移进行概述,以期望为大肠杆菌的致病机理及大肠杆菌病的防控提供帮助。

关键词: 人兽共患病病原; 致病性大肠杆菌; HPI毒力岛; 水平转移

Abstract: Pathogenic Escherichia coli (E.coli) as a member of Enterobacteriaceae and one of the zoonotic pathogens,can cause human and animal infections through a variety of ways and cause different E.coli disease types under certain conditions.Due to the large number of serotypes and virulence factors,the severe trend of drug resistance,the existence of biofilm and other factors,the epidemic trend of colibacillosis is complex and the development of vaccines is difficult of pathogenic E.coli,which brings certain economic losses to the domestic and foreign livestock and poultry breeding industry.High-pathogenicity island (HPI) was first discovered in Yersinia,which was proved to be a genomic island necessary for its virulence phenotype.Due to the horizontal transfer mechanism of bacteria,the virulence island is widely detected in other Enterobacteriaceae.Among them,the HPI pathogenicity island was also detected in pathogenic E.coli such as enterohemorrhage E.coli (EHEC) and shiga toxin-producing E.coli (STEC),which was confirmed to affect the virulence of pathogenic E.coli,participate in the iron uptake of pathogenic E.coli and mediate the host immune response.In order to further understand the iron regulation mechanism,pathogenic characteristics,mediated cellular immune response and horizontal transfer pathway of HPI virulence island in pathogenic E.coli,this paper summarizes the structure of HPI virulence island,how to regulate Yersinia siderophore,the pathogenicity of HPI virulence island and its regulation of cellular immune response,and the horizontal transfer of HPI virulence island.It is expected to provide help for the pathogenic mechanism of E.coli and the prevention and control of E.coli disease.

Key words: zoonotic pathogen; pathogenic Escherichia coli; HPI virulence island; horizontal transfer

中图分类号:

S855.1

邹宏, 罗干, 陈玲, 杜鹏飞, 张乾义, 杨承槐, 田野. 致病性大肠杆菌HPI毒力岛及其水平转移的研究进展[J]. 中国畜牧兽医, 2023, 50(8): 3430-3437.

ZOU Hong, LUO Gan, CHEN Ling, DU Pengfei, ZHANG Qianyi, YANG Chenghuai, TIAN Ye. Research Progress on HPI Virulence Island and Its Horizontal Transfer in Pathogenic Escherichia coli[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3430-3437.

参考文献

[1] WIRTH T,FALUSH D,LAN R,et al.Sex and virulence in Escherichia coli:An evolutionary perspective[J].Molecular Microbiology,2006,60(5):1136-1151.
[2] ALLOCATI N,MASULLI M,ALEXEYEV M F,et al.Escherichia coli in Europe:An overview[J].International Journal of Environmental Research and Public Health,2013,10(12):6235-6254.
[3] LEIMBACH A,HACKER J,DOBRINDT U.E.coli as an all-rounder:The thin line between commensalism and pathogenicity[J].Current Topics in Microbiology and Immunology,2013,358:3-32.
[4] GRASS G.Iron transport in Escherichia coli:All has not been said and done[J].Biometals,2006,19(2):159-172.
[5] SCHUBERT S,RAKIN A,HEESEMANN J.The Yersinia high-pathogenicity island (HPI):Evolutionary and functional aspects[J].International Journal of Medical Microbiology,2004,294(2-3):83-94.
[6] MARTIN P,TRONNET S,GARCIE C,et al.Interplay between siderophores and colibactin genotoxin in Escherichia coli[J].IUBMB Life,2017,69(6):435-441.
[7] SHAN C L,MIAO S S,LIU C Y,et al.Induction of macrophage pyroptosis-related factors by pathogenic E.coli high pathogenicity island (HPI) in Yunnan Saba pigs[J].BMC Veterinary Research,2021,17:114.
[8] KOCZURA R,MOKRACKA J,KAZNOWSKI A.The Yersinia high-pathogenicity island in Escherichia coli and Klebsiella pneumoniae isolated from polymicrobial infections[J].Polish Journal of Microbiology,2012,61(1):71-73.
[9] SCHUBERT S,RAKIN A,HEESEMANN J.Characterization of the integration site of Yersinia high-pathogenicity island in Escherichia coli[J].FEMS Microbiology Letters,1999,179(2):409-414.
[10] XU J G,CHENG B K,WEN X Q,et al.High-pathogenicity island of Yersinia spp.in Escherichia coli strains isolated from diarrhea patients in China[J].Journal of Clinical Microbiology,2000,38(12):4672-4675.
[11] TU J,QI K Z,SONG X,et al.Horizontal transfer and functional evaluation of high pathogenicity islands in Avian Escherichia coli[J].Polish Journal of Veterinary Sciences,2017,20(2):395-402.
[12] JIAN T,TING X,KE Q Q,et al.The irp2 and fyuA genes in high pathogenicity islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli(APEC)[J].Polish Journal of Veterinary Sciences,2016,19(1):21-29.
[13] CLERMONT O,BONACORSI S,BINGEN E.The Yersinia high-pathogenicity island is highly predominant in virulence-associated phylogenetic groups of Escherichia coli[J].FEMS Microbiology Letters,2001,196(2):153-157.
[14] BRAUN V.Iron uptake by Escherichia coli[J].Frontiers in Bioscience,2003,8:s1409-1421.
[15] MILLER M C,FETHERSTON J D,PICKETT C L,et al.Reduced synthesis of the Ybt siderophore or production of aberrant Ybt-like molecules activates transcription of yersiniabactin genes in Yersinia pestis[J].Microbiology,2010,156(Pt 7):2226-2238.
[16] GARÉNAUX A,CAZA M,DOZOIS C M.The ins and outs of siderophore mediated iron uptake by extra-intestinal pathogenic Escherichia coli[J].Veterinary Microbiology,2011,153(1-2):89-98.
[17] LAM M M C,WYRES K L,JUDD L M,et al.Tracking key virulence loci encoding aerobactin and salmochelin siderophore synthesis in Klebsiella pneumoniae[J].Genome Medicine,2018,10(1):77.
[18] BOBROV A G,GEOFFROY V A,PERRY R D.Yersiniabactin production requires the thioesterase domain of HMWP2 and YbtD,a putative phosphopantetheinylate transferase[J].Infection and Immunity,2002,70(8):4204-4214.
[19] ANISIMOV R,BREM D,HEESEMANN J,et al.Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica[J].FEMS Microbiology Letters,2005,250(1):27-32.
[20] PERRY R D,FETHERSTON J D.Yersiniabactin iron uptake:Mechanisms and role in Yersinia pestis pathogenesis[J].Microbes and Infection,2011,13(10):808-817.
[21] GEHRING A M,BRADLEY K A,WALSH C T.Enterobactin biosynthesis in Escherichia coli:Isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate[J]. Biochemistry,1997,36(28):8495-8503.
[22] RAYMOND K N,DERTZ E A,KIM S S.Enterobactin:An archetype for microbial iron transport[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(7):3584-3588.
[23] XU G,GUO H,LV H T.Metabolomics assay identified a novel virulence-associated siderophore encoded by the high-pathogenicity island in uropathogenic Escherichia coli[J]. Journal of Proteome Research,2019,18(5):2331-2336.
[24] KLEBBA P E,NEWTON S M C,SIX D A,et al.Iron acquisition systems of Gram-negative bacterial pathogens define TonB-dependent pathways to novel antibiotics[J].Chemical Reviews,2021,121(9):5193-5239.
[25] HU J,KAN B,LIU Z H,et al.Enteroaggregative Escherichia coli isolated from Chinese diarrhea patients with high-pathogenicity island of Yersinia is involved in synthesis of Siderophore yersiniabactin[J].World Journal of Gastroenterology,2005,11(37):5816-5820.
[26] MAJUMDAR A,TRINH V,MOORE K J,et al.Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport[J]. The Journal of Biological Chemistry,2020,295(15):4974-4984.
[27] GRESOCK M G,POSTLE K.Going outside the TonB box:Identification of novel FepA-TonB interactions in vivo[J].Journal of Bacteriology,2017,199(10):e00649-16.
[28] SU Q,GUAN T B,HE Y,et al.Siderophore biosynthesis governs the virulence of uropathogenic Escherichia coli by coordinately modulating the differential metabolism[J]. Journal of Proteome Research,2016,15(4):1323-1332.
[29] JU W T,SHEN J L,TORO M,et al.Distribution of pathogenicity islands OI-122,OI-43/48,and OI-57 and a high-pathogenicity island in shiga toxin-producing Escherichia coli[J].Applied and Environmental Microbiology,2013,79(11):3406-3412.
[30] MAGISTRO G,MAGISTRO C,STIEF C G,et al.The high-pathogenicity island (HPI) promotes flagellum-mediated motility in extraintestinal pathogenic Escherichia coli[J]. PLoS One,2017,12(10):e0183950-18.
[31] STEIN J,HARTMANN F,DIGNASS A U.Diagnosis and management of iron deficiency anemia in patients with IBD[J].Nature Reviews.Gastroenterology&Hepatology,2010,7(11):599-610.
[32] YU P,ZHANG X,LIU N,et al.Pyroptosis:Mechanisms and diseases[J].Signal Transduction and Targeted Therapy,2021,6(1):128-149.
[33] ZHAO W W,GAO B,LIU C,et al.High pathogenicity island is associated with enhanced autophagy in pathogenic Escherichia coli HPI-infected macrophages[J]. Research in Veterinary Science,2021,135:113-120.
[34] DALMASSO G,NGUYEN H T T,FAÏS T,et al.Yersiniabactin siderophore of Crohn's disease-associated adherent-invasive Escherichia coli is involved in autophagy activation in host cells[J].International Journal of Molecular Sciences,2021,22(7):3512-3517.
[35] BRETIN A,CARRIōRE J,DALMASSO G,et al.Activation of the EIF2AK4-EIF2A/eIF2α-ATF4 pathway triggers autophagy response to Crohn disease-associated adherent-invasive Escherichia coli infection[J].Autophagy, 2016,12(5):770-783.
[36] HARTMANN H,ELTZSCHIG H K,WURZ H,et al.Hypoxia-independent activation of HIF-1 by enterobacteriaceae and their siderophores[J]. Gastroenterology,2008,134(3):756-767.
[37] DALMASSO G,NGUYEN H T T,FAÏS T,et al.Crohn's disease-associated adherent-invasive Escherichia coli manipulate host autophagy by impairing SUMOylation[J].Cells,2019,8(1):35.
[38] KOH E I,ROBINSON A E,BANDARA N,et al.Copper import in Escherichia coli by the yersiniabactin metallophore system[J].Nature Chemical Biology,2017,13(9):1016-1021.
[39] SCHUBERT S,DARLU P,CLERMONT O,et al.Role of intraspecies recombination in the spread of pathogenicity islands within the Escherichia coli species[J]. PLoS Pathogens,2009,5(1):e1000257-10.
[40] MESSERER M,FISCHER W,SCHUBERT S.Investigation of horizontal gene transfer of pathogenicity islands in Escherichia coli using next-generation sequencing[J].PLoS One,2017,12(7):e0179880-17.
[41] ANTONENKA U,NÖLTING C,HEESEMANN J,et al.Horizontal transfer of Yersinia high-pathogenicity island by the conjugative RP4 attB target-presenting shuttle plasmid[J].Molecular Microbiology,2005,57(3):727-734.
[42] BACH S,DE ALMEIDA A,CARNIEL E.The Yersinia high-pathogenicity island is present in different members of the family Enterobacteriaceae[J].FEMS Microbiology Letters,2000,183(2):289-294.
[43] LESIC B,BACH S,GHIGO J M,et al.Excision of the high-pathogenicity island of Yersinia pseudotuberculosis requires the combined actions of its cognate integrase and Hef,a new recombination directionality factor[J]. Molecular Microbiology,2004,52(5):1337-1348.
[44] SCHUBERT S,DUFKE S,SORSA J,et al.A novel integrative and conjugative element (ICE) of Escherichia coli:The putative progenitor of the Yersinia high-pathogenicity island[J].Molecular Microbiology,2004,51(3):837-848.
[45] HACKER J,CARNIEL E.Ecological fitness,genomic islands and bacterial pathogenicity.A Darwinian view of the evolution of microbes[J].EMBO Reports,2001,2(5):376-381.
[46] SCHUBERT S,PICARD B,GOURIOU S,et al.Yersinia high-pathogenicity island contributes to virulence in Escherichia coli causing extraintestinal infections[J].Infection and Immunity,2002,70(9):5335-5337.
[47] 郭瑞亮,张刚,崔钦娜,等.细菌中基因组岛的转移与调控机制研究进展[J].微生物学通报,2018,45(10):2234-2242. GUO R L,ZHANG G,CUI Q N,et al.Regulation,excision and horizontal transfer of genomic islands in bacteria[J].Microbiology China,2018,45(10):2234-2242.(in Chinese)
[48] CHAKRABORTI S,CHAKRABARTI P.Self-assembly of ferritin:Structure,biological function and potential applications in nanotechnology[J].Advances in Experimental Medicine and Biology,2019,1174:313-329.
[49] MAGYAR T,GYURISÉ,UJVÁRI B,et al.Genotyping of Riemerella anatipestifer by ERIC-PCR and correlation with serotypes[J]. Avian Pathology:Journal of the W.V.P.A,2019,48(1):12-16.
[50] LA RAGIONE R M,WOODWARD M J.Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia[J].Research in Veterinary Science,2002,73(1):27-35.
[51] SHARMA G,SHARMA S,SHARMA P,et al.Escherichia coli biofilm:Development and therapeutic strategies[J].Journal of Applied Microbiology,2016,121(2):309-319.
[52] JANG J,HUR H G,SADOWSKY M J,et al.Environmental Escherichia coli:Ecology and public health implications-A review[J].Journal of Applied Microbiology,2017,123(3):570-581.