河南省猪伪狂犬病病毒野毒感染血清学调查及gE基因遗传变异分析

doi:10.16431/j.cnki.1671-7236.2023.08.034

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (8): 3364-3372.doi: 10.16431/j.cnki.1671-7236.2023.08.034

• 预防兽医 • 上一篇

河南省猪伪狂犬病病毒野毒感染血清学调查及gE基因遗传变异分析

王林青^1,2, 赵丽³, 陈曦艋¹, 吴少峰⁴, 韩莹莹¹, 刘芳¹, 金钺¹, 陈红英¹

1. 河南农业大学动物医学院, 郑州 450046;
2. 郑州师范学院分子生物学郑州市重点实验室, 郑州 450044;
3. 河南牧业经济学院动物医药学院, 郑州 450046;
4. 河南农业大学动物科技学院, 郑州 450046

收稿日期:2023-02-17 发布日期:2023-07-27
通讯作者: 金钺, 陈红英 E-mail:jinyue2020@163.com;chhy927@163.com
作者简介:王林青,E-mail:wanglintsing@163.com;赵丽,E-mail:zhaolisq@163.com。
基金资助:
河南省科技攻关项目(222102110053、222102110332);河南省自然科学基金面上项目(222300420586);河南省高校科技创新人才支持计划(21HASTIT039);河南牧业经济学院博士启动基金(2020HNUAHEDF010)

Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province

WANG Linqing^1,2, ZHAO Li³, CHEN Ximeng¹, WU Shaofeng⁴, HAN Yingying¹, LIU Fang¹, JIN Yue¹, CHEN Hongying¹

1. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China;
2. Zhengzhou Key Laboratory of Molecular Biology, Zhengzhou Normal University, Zhengzhou 450044, China;
3. College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
4. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China

Received:2023-02-17 Published:2023-07-27

摘要/Abstract

摘要： 【目的】调查河南省猪伪狂犬病病毒(Pseudorabies virus,PRV)野毒流行特征及遗传变异情况。【方法】用ELISA法检测2020—2021年从河南省766个不同养殖规模猪场采集的25 411份血清样本的gE抗体水平;采用PCR法对从47个猪场疑似PRV感染猪群中收集的251份病料进行gE基因扩增,并将阳性病料样品接种ST细胞进行病毒分离、gE全基因测序和遗传进化分析。【结果】血清样品gE抗体总阳性率为18.42%,从2020年的20.32%降至2021年的16.69%,猪场阳性率为50.26%。随着猪场规模增大,猪场阳性率、血清样本阳性率呈下降趋势;商品代养殖场、屠宰场和种猪场的场阳性率、血清样本阳性率依次降低;豫东、豫西、豫南、豫北和豫中血清gE抗体阳性率分别为15.05%、23.48%、16.50%、16.69%和20.10%,1-3月阳性率最高,10-12月最低。组织病料样品中PRV阳性率为15.14%(38/251),从PRV阳性病料样品中共分离出9株PRV分离株。gE全基因序列遗传进化分析结果显示,分离株都属于GenotypeⅡ型,且猪群中既有PRV经典株也有PRV变异株。【结论】河南省猪场中仍然存在PRV感染,且同时存在PRV经典株和PRV变异株,本研究结果为河南省猪伪狂犬病的防控与净化提供参考依据。

关键词: 猪; 伪狂犬病病毒(PRV); 血清学调查; gE基因; 遗传变异

Abstract: 【Objective】 The purpose of this study was to investigate the epidemiological characteristics and genetic variation of Pseudorabies virus (PRV) in swine in Henan Province.【Method】 gE antibody levels were detected by ELISA in 25 411 serum samples collected from 766 pig farms in Henan Province during 2020-2021.gE gene was amplified by PCR in 251 samples from 47 pig farms suspected of PRV-infected pigs,and the positive samples were inoculated with ST cells for virus isolation,gE gene sequencing and genetic evolution analysis.【Result】 The total positive rate of gE antibody in serum samples was 18.42%,which decreased from 20.32% in 2020 to 16.69% in 2021.The positive rate in pig farms was 50.26%.With the increase of pig farm size, the positive rate of farm and serum sample showed a downward trend. The farm and serum positive rate in commodity farm, slaughterhouse and pig breeding farm decreased successively. The positive rates of gE antibody in eastern, western, southern, northern and central Henan were 15.05%, 23.48%, 16.50%, 16.69% and 20.10%, respectively. The positive rates were the highest from January to March and the lowest from October to December.The positive rate of PRV in tissue samples was 15.14% (38/251).A total of 9 PRV isolates were isolated from PRV positive samples.Phylogenetic analysis based on the gE complete sequences indicated that all isolates belonged to Genotype Ⅱ and there were both classical strains and variant strains in pig herds.【Conclusion】 PRV classical strains and PRV variant strains still existed in pig farms in Henan province.The results provided reference for the prevention and control and purification of PRV in Henan province.

Key words: pig; Pseudorabies virus(PRV); serological investigation; gE gene; genetic variation

中图分类号:

S852.65⁺1

王林青, 赵丽, 陈曦艋, 吴少峰, 韩莹莹, 刘芳, 金钺, 陈红英. 河南省猪伪狂犬病病毒野毒感染血清学调查及gE基因遗传变异分析[J]. 中国畜牧兽医, 2023, 50(8): 3364-3372.

WANG Linqing, ZHAO Li, CHEN Ximeng, WU Shaofeng, HAN Yingying, LIU Fang, JIN Yue, CHEN Hongying. Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3364-3372.

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河南省猪伪狂犬病病毒野毒感染血清学调查及gE基因遗传变异分析

Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province

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