牦牛产色葡萄球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2023.07.041

摘要/Abstract

摘要： 【目的】了解西藏拉萨地区牦牛源产色葡萄球菌的致病性及耐药性情况，以期为牦牛感染产色葡萄球菌的防治提供一定参考。【方法】采用细菌分离培养、生化试验鉴定、16S rDNA测序、相似性比对分析的方法对从西藏拉萨地区采集到的65份牦牛样品中的产色葡萄球菌进行鉴定，并研究其耐药性与致病性。【结果】在65份样品中共获得9株分离菌，分别命名为XZ1~XZ9，分离率为13.85%；分离菌均在5%绵羊血平板上呈淡黄色的小菌落，革兰氏染色为阳性球菌；生化试验鉴定显示，分离菌株对甘露糖、果糖、蔗糖、硝酸盐还原反应为阳性，符合产色葡萄球菌特性；经16S rDNA测序、相似性比对分析发现，9株分离菌均与产色葡萄球菌相似性最高，达96%以上；药敏试验结果显示，9株分离菌最多对3种抗菌药耐药，最少对1种抗菌药耐药；用分离菌株对SPF小鼠进行攻毒试验发现，分离菌对小鼠的致病性较强，导致小鼠肺脏及肾脏淤血、肿大，肠道发生病变，出现黄色稀便。【结论】本研究结果表明，牦牛源产色葡萄球菌在拉萨地区的牦牛中存在一定的致病性，且耐药性较强，应当引起一定的重视。

关键词: 牦牛; 产色葡萄球菌; 分离鉴定

Abstract: 【Objective】 This experiment was aimed to investigate the pathogenicity and drug resistance of Staphylococcus chromogenes isolated from yaks in Lhasa, Tibet, so as to provide some reference for the prevention and treatment of Staphylococcus chromogenes infection in yaks.【Method】 Bacterial isolation and culture, biochemical test identification, 16S rDNA sequencing and similarity comparison analysis were used to identify Staphylococcus chromogenes from 65 yak samples collected from Lhasa, Tibet, and to study their drug resistance and pathogenicity.【Result】 A total of 9 isolates were obtained from 65 samples, which were named XZ1-XZ9 and the isolation rate was 13.85%.All the isolates showed small yellow colonies on 5% sheep blood plate and were positive by Gram staining.Biochemical test showed that the isolated strains were positive for mannose, fructose, sucrose and nitrate reduction, and the results were consistent with the characteristics of Staphylococcus chromogenes.Through 16S rDNA sequencing and similarity comparison analysis, it was found that the 9 isolates had the highest homology with Staphylococcus chromogenes, which was more than 96%.The results of drug sensitivity test showed that 9 strains were most resistant to three antibiotics and at least resistant to one antibiotic.The bacterial challenge test was carried out on SPF mice with the isolated strains.It was found that the isolated bacteria had strong pathogenicity to mice, resulting in congestion and swelling of the lungs and kidneys, intestinal lesions and yellow thin stools.【Conclusion】 The results of this study showed that Staphylococcus chromogenes isolated from yaks in Lhasa area had a certain pathogenicity and strong drug resistance, which should be paid more attention.

Key words: yak; Staphylococcus chromogenes; isolation and identification

中图分类号:

S852.61

邹明昊, 马弘财, 王冰艺, 樊世杰, 曾江勇, 吴庆侠. 牦牛产色葡萄球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(7): 3017-3025.

ZOU Minghao, MA Hongcai, WANG Bingyi, FAN Shijie, ZENG Jiangyong, WU Qingxia. Isolation, Identification and Drug Resistance Analysis of Staphylococcus chromogenes in Yak[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 3017-3025.

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