新疆地区2株驴源马流产沙门菌的分离鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2022.01.042

摘要/Abstract

摘要： [目的] 研究确定导致新疆地区2个规模化驴场出现流产的疑似病原沙门菌，并探究其致病能力和耐药性情况。[方法] 通过细菌分离培养、形态学观察、生化试验对分离菌进行了鉴定，并对分离菌的鞭毛基因FliC进行了PCR扩增及序列分析，通过致病性测定、荷菌量检测及病理组织学观察，鉴定和分析了分离菌的致病性，并通过药敏试验分析其耐药性。[结果] 通过细菌分离培养、形态学观察、生化试验，确定分离到的2株细菌均为马流产沙门菌，分别命名为G1-1和XD1-2。对这2个分离株的鞭毛基因FliC的遗传进化分析结果显示，2株分离菌FliC氨基酸序列之间的相似性为99.0%，2株分离菌FliC氨基酸序列与爱尔兰马源马流产沙门菌分离株Ireland-HE801373、Ireland-HE801378株相似性均最高，且均为99.3%；分离株G1-1与爱尔兰马源马流产沙门菌分离株Ireland-HE801373和Ireland-HE801378及国内马源马流产沙门菌分离株China-KJ486797.1和China-KJ486769.1亲缘关系较近，分离株XD1-2则与美国肠炎沙门菌分离株USA-EBQ1214032.1亲缘关系较近，相似性分析与进化树结果一致。小鼠致病性试验显示，2株分离菌对小鼠的毒性均较强且致病性存在差异，XD1-2对小鼠的致病性强于G1-1。分离菌G1-1对9种抗菌药物耐药，对11种抗菌药物敏感。分离菌XD1-2对11种抗菌药物耐药，对5种抗菌药物敏感。[结论] 本试验成功分离到2株驴源沙门菌，不同养殖场来源的驴源沙门菌的致病能力和耐药性有一定差异，对鞭毛基因FliC的进化分析也显示出差异，本研究结果为驴源沙门菌的防治提供了一定的参考依据。

关键词: 驴源马流产沙门菌; 分离鉴定; FliC基因; 致病性

Abstract: [Objective] The suspected pathogenic Salmonella causing abortion in two large-scale donkey farms in Xinjiang was determined, and its pathogenicity and drug resistance were explored. [Method] In this experiment, the isolated bacteria were identified by bacterial isolation and culture, morphological observation, physiological and biochemical test, and the flagella gene FliC of the isolated bacteria was amplified by PCR and sequenced. The pathogenicity of the isolated bacteria was identified and analyzed by pathogenicity determination, bacterial load detection and histopathological observation, and its drug resistance was analyzed by drug sensitivity test. [Result] Through bacterial isolation and culture, morphological observation and physiological and biochemical test, it was determined that the two strains of bacteria were Salmonella abortus equi and named G1-1 and XD1-2, respectively. The genetic evolution analysis of flagellum gene FliC of the two isolates showed that the similarity of FliC amino acid sequences between the two isolates was 99.0%, and the similarity between FliC amino acid sequences of the two isolates and Salmonella abortus equi isolates Ireland-HE801373 and Ireland-HE801378 was the highest, both of which were 99.3%. The isolates G1-1 was closely related to Ireland-HE801373, Ireland-HE801378, China-KJ486797.1 and China-KJ486769.1, and the isolates XD1-2 was closely related to USA-EBQ1214032.1. The similarity analysis were consistent with the phylogenetic tree results. The mouse pathogenicity test showed that the two isolates had strong toxicity to mice and different pathogenicity. The pathogenicity of XD1-2 to mice was stronger than that of G1-1. The isolated strain XD1-2 was resistant to 11 kinds of antibiotics and sensitive to 5 kinds of antibiotics. The isolated strain G1-1 was resistant to 9 kinds of antibiotics and sensitive to 11 kinds of antibiotics. [Conclusion] In this study, two strains of Salmonella abortus equi from different donkey farms were successfully isolated and identified, which had different drug sensitivity and virulence. In addition, the genetic evolution analysis result indicated that they were genetic different, and the evolutionary analysis of flagella gene FLIC also showed differences. The results provided a reference for the prevention and treatment of Salmonella abortus equi in donkey.

Key words: Salmonella abortus equi from donkey; isolation and identification; FliC gene; pathogenicity

中图分类号:

S855.1⁺2

蒲小峰, 张雨薇, 李胜男, 张雪静, 苏艳. 新疆地区2株驴源马流产沙门菌的分离鉴定及致病性分析[J]. 中国畜牧兽医, 2022, 49(1): 395-403.

PU Xiaofeng, ZHANG Yuwei, LI Shengnan, ZHANG Xuejing, SU Yan. Isolation, Identification and Pathogenicity Analysis of Two Salmonella abortus equi Strains from Donkeys in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 395-403.

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