亲代三硝基丙酸诱导的氧化应激对胎鼠的影响

doi:10.16431/j.cnki.1671-7236.2021.06.040

摘要/Abstract

摘要： 试验旨在研究氧化应激对子代小鼠产生的影响。对亲代的公鼠和母鼠分别腹腔注射12.5 μg/g三硝基丙酸（3-NPA），分为正常公鼠与正常母鼠交配的对照组（C）、正常公鼠与3-NPA处理的母鼠交配组（FM）、正常母鼠与3-NPA处理的公鼠交配组（MM）、3-NPA处理的公鼠与3-NPA处理的母鼠交配组（DM）共4组，试验重复3次。通过剖出胎鼠的方法统计子代胎鼠的数量，采集子代胎鼠的心脏、肝脏、肺脏3个器官，测定超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）的活力及总抗氧化能力（T-AOC）。结果显示，与对照组相比，FM组胎鼠数量下降不明显（P>0.05），MM和DM组胎鼠数量均显著减少（P<0.05）；FM与DM组胎鼠肝脏、肺脏中CAT活力均显著增加（P<0.05）；MM组胎鼠肝脏、肺脏中CAT活力增加，但差异不显著（P>0.05）；各组心脏中CAT活力均增加，但差异均不显著（P>0.05）；除MM组肺脏中T-AOC活力增加差异不显著（P>0.05），其余各组肝脏、肺脏和心脏中T-AOC活力均显著增加（P<0.05）；DM组胎鼠肝脏中GSH-Px活力显著增加（P<0.05），FM和MM组胎鼠肝脏中GSH-Px活力增加，但差异不显著（P>0.05）；FM与DM组胎鼠肺脏中GSH-Px活力显著升高（P<0.05）；各组心脏中GSH-Px活力均差异不显著（P>0.05）；DM组胎鼠肝脏、肺脏、心脏中SOD活力均显著增加（P<0.05），FM和MM组胎鼠肝脏、肺脏、心脏中SOD活力增加，但差异均不显著（P>0.05）。因此，试验成功构建3-NPA氧化应激小鼠模型，且亲代雄性小鼠氧化应激对胎鼠影响较大。

关键词: 胎鼠; 器官; 氧化应激; 三硝基丙酸; 抗氧化

Abstract: This experiment was aimed to study the effects of oxidative stress on the offspring of mice.The male and female mice were injected 12.5 μg/g 3-nitropropionic acid (3-NPA) intraperitoneally,which were divided into normal male mice and normal female mice of control group (C),normal male and the 3-NPA treated female (FM),normal female and the 3-NPA treated male mice (MM) and 3-NPA treated male mice and the 3-NPA treated female mice mating group (DM) for 3 times.The number of progeny fetal mice was counted by the method of secting fetal mice.The heart,liver and lung of the progeny fetal mice were collected and the activities of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) of the progeny fetal mice were determined.The results showed that compared with the control group,the number of fetal mice in FM group was not significantly decreased (P>0.05),while the number of fetal mice in MM and DM groups was significantly decreased (P<0.05).The CAT activity in liver and lung of fetal mice in FM and DM groups was significantly increased (P<0.05).The CAT activity in liver and lung of fetal mice in MM group was increased,but the difference was not significant (P>0.05).CAT activity in heart increased in all groups,but there was no significant difference (P>0.05).T-AOC activity in liver,lung and heart was significantly increased (P<0.05),except for in lung in MM group (P>0.05).The activity of GSH-Px in fetal liver of DM group was significantly increased (P<0.05),and increased in FM and MM groups,but the difference was not significant (P>0.05).The activity of GSH-Px in lung of fetal mice in FM and DM groups was significantly increased (P<0.05).There was no significant difference in GSH-Px activity among all groups in heart (P>0.05).The activity of SOD in liver,lung and heart of fetal mice in DM group was significantly increased (P<0.05),and increased in liver,lung and heart in FM and MM groups,but the differences were not significant (P>0.05).Therefore,the oxidative stress mouse model of 3-NPA was successfully established in this study,and the oxidative stress of parental male mice had a greater effect on fetal mice.

Key words: fetal mice; organ; oxidative stress; 3-nitropropionic acid; antioxidation

中图分类号:

S814

高见红, 于佳彤, 王慧瑞, 刘旭, 郭庸, 方南洙, 柳海星. 亲代三硝基丙酸诱导的氧化应激对胎鼠的影响[J]. 中国畜牧兽医, 2021, 48(6): 2255-2261.

GAO Jianhong, YU Jiatong, WANG Huirui, LIU Xu, GUO Yong, FANG Nanzhu, LIU Haixing. Effects of Oxidative Stress Induced by Parental 3-nitropropionic Acid on the Fetal Mice[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2255-2261.

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