阳离子脂质体包埋角鲨烯佐剂的制备及理化性质研究

doi:10.16431/j.cnki.1671-7236.2021.04.031

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (4): 1431-1439.doi: 10.16431/j.cnki.1671-7236.2021.04.031

阳离子脂质体包埋角鲨烯佐剂的制备及理化性质研究

万蒙¹, 陈懿琳¹, 钱宇豪¹, 黄选¹, 夏廉臣¹, 王春维^1,2, 祝爱侠^1,2

1. 武汉轻工大学动物科学与营养工程学院, 武汉 430023;
2. 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉 430023

收稿日期:2020-08-07 出版日期:2021-04-20 发布日期:2021-04-16
通讯作者: 祝爱侠 E-mail:415593244@qq.com
作者简介:万蒙(1994-),女,湖北孝感人,硕士,研究方向:饲料添加剂及资源开发与利用,E-mail:314135207@qq.com
基金资助:
国家重点研发计划项目（2017YFD0500603）；动物营养与饲料科学湖北省重点实验室开放课题资助（DKXY2020012）

Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties

WAN Meng¹, CHEN Yilin¹, QIAN Yuhao¹, HUANG Xuan¹, XIA Lianchen¹, WANG Chunwei^1,2, ZHU Aixia^1,2

1. College of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
2. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan University of Light Industry, Wuhan 430023, China

Received:2020-08-07 Online:2021-04-20 Published:2021-04-16

摘要/Abstract

摘要： 为研究一种阳离子脂质体包埋角鲨烯佐剂的制备工艺，试验采用乙醇注入法结合探头超声，以卵磷脂、胆固醇和硬脂胺为原料包被具有免疫功能作用的可代谢油脂角鲨烯，以粒径、稳定性指数（TSI）和Zeta电位为评价指标进行优化研究，并对阳离子脂质体包被角鲨烯佐剂理化性质进行分析。结果表明，最佳阳离子脂质体包被角鲨烯佐剂制备条件为：卵磷脂15 mg/mL，胆固醇2.0 mg/mL，角鲨烯8 μL/mL，探头超声时间15 min，超声功率100 W。在此条件下所制佐剂平均电位45 mV，平均粒径182 nm，黏度<80 mPa·s，其粒度小、黏度小、性能稳定，具有进一步作为疫苗佐剂的研究价值。

关键词: 阳离子脂质体; 角鲨烯; 佐剂; 黏度

Abstract: To study the preparation process of a cationic liposome-embedded squalene adjuvant,and the lecithin,cholesterol and steary lamine were used as raw materials to coat metabolizable lipid squalene with immune function,using the method of ethanol injection combined with probe ultrasound in this experiment.Furthermore,the particle size,stability index (TSI) and Zeta potential were used as evaluation indexes and the physical and chemical properties of cationic liposome-coated squalene adjuvant were analyzed.The results showed that the optimal preparation conditions of cationic liposome-coated squalene adjuvant were lecithin 15 mg/mL,cholesterol 2.0 mg/mL,squalene 8 μL/mL,probe ultrasonic time 15 min and ultrasonic power 100 W.Under the optimum conditions,the adjuvant could be obtained with an average potential of 45 mV,an average particle size of 182 nm,and a viscosity of less than 80 mPa·s,meanwhile,it also had a great significance in further researching as a vaccine adjuvant with small particle size,low viscosity and stable performance.

Key words: cationic liposome; squalene; adjuvant; viscosity

中图分类号:

S816.8

万蒙, 陈懿琳, 钱宇豪, 黄选, 夏廉臣, 王春维, 祝爱侠. 阳离子脂质体包埋角鲨烯佐剂的制备及理化性质研究[J]. 中国畜牧兽医, 2021, 48(4): 1431-1439.

WAN Meng, CHEN Yilin, QIAN Yuhao, HUANG Xuan, XIA Lianchen, WANG Chunwei, ZHU Aixia. Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1431-1439.

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阳离子脂质体包埋角鲨烯佐剂的制备及理化性质研究

Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties

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