猪瘟活疫苗佐剂稀释剂的制备及免疫效果评价

doi:10.16431/j.cnki.1671-7236.2015.09.037

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (9): 2467-2473.doi: 10.16431/j.cnki.1671-7236.2015.09.037

猪瘟活疫苗佐剂稀释剂的制备及免疫效果评价

梁孔贤¹, 吕晓君², 丁丹¹, 许琦³, 周杰², 刘卫^1,4

1. 华中科技大学生命科学与技术学院, 武汉 430074;
2. 湖北省食品药品监督检验研究院, 武汉 430074;
3. 武汉百思凯瑞纳米科技有限公司, 武汉 430074;
4. 华中科技大学国家纳米药物技术研究中心, 武汉 430074

收稿日期:2015-03-16 出版日期:2015-09-20 发布日期:2015-09-25
通讯作者: 刘卫 E-mail:wliu@hust.edu.cn
作者简介:梁孔贤(1987-),男,甘肃武威人,硕士生,研究方向:纳米药物制剂,E-mail:liangkongxian@163.com
基金资助:
国家自然科学基金(31470968)

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

LIANG Kong-xian¹, LV Xiao-jun², DING Dan¹, XU Qi³, ZHOU Jie², LIU Wei^1,4

1. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;
2. Hubei Institute for Food and Drug Control, Wuhan 430074, China;
3. Wuhan Best-carrier Nano Science and Technology Co., Ltd., Wuhan 430074, China;
4. National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China

Received:2015-03-16 Online:2015-09-20 Published:2015-09-25

摘要/Abstract

摘要： 本试验旨在针对性的研发一种安全性好、能有效增强免疫效果、使用方便的猪瘟活疫苗新型佐剂稀释剂(AD),并对AD的免疫增强效果进行评价。采用高压均质技术制备了AD,采用响应面试验设计优化了AD的处方和制备工艺,并对其稳定性、粒径、多分散指数(PDI)及Zeta电位进行表征。将AD与猪瘟活疫苗混合后,肌肉注射免疫BALB/c小鼠,进行佐剂增强免疫效果评价。优化处方和工艺制备的AD平均粒径为100.4 nm,PDI为0.147,Zeta电位为—28.7 mV,试验结果表明其稳定性良好。免疫效果评价试验结果显示,佐剂组小鼠血清中抗猪瘟病毒(CSFV)抗体水平与对照组相比有显著差异(P<0.05),抗体水平能持续较长时间,表明AD能有效诱导机体产生体液免疫应答;佐剂组小鼠血清中IL-4、IL-6和IFN-γ的水平均有明显提高,表明该疫苗佐剂能诱导机体产生细胞免疫应答。本试验制备的佐剂稀释剂与疫苗混合使用,能显著提高体液免疫和细胞免疫水平,从而增强疫苗的免疫刺激能力。

关键词: 猪瘟活疫苗; 佐剂稀释剂; 高压均质技术; 响应面设计

Abstract: The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.

Key words: live vaccine against classical swine fever; adjuvant diluents (AD); high-pressure homogenization technique; response surface design

中图分类号:

S859.79

梁孔贤, 吕晓君, 丁丹, 许琦, 周杰, 刘卫. 猪瘟活疫苗佐剂稀释剂的制备及免疫效果评价[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2467-2473.

LIANG Kong-xian, LV Xiao-jun, DING Dan, XU Qi, ZHOU Jie, LIU Wei. Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects[J]. , 2015, 42(9): 2467-2473.

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猪瘟活疫苗佐剂稀释剂的制备及免疫效果评价

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

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