小分子化合物单克隆抗体的制备及其在动物医学领域的应用

doi:10.16431/j.cnki.1671-7236.2021.03.037

摘要/Abstract

摘要： 农药、兽药、重金属、生物毒素等残留物质的痕量检测在医学诊断、食品安全、环境监测等领域均具有重要意义，其中基于抗原-抗体特异性识别的免疫分析技术具有特异性强、灵敏度高、稳定性好、操作简便快捷等优点，但如何获得小分子化合物的特异性抗体是研制免疫分析技术的关键。作者介绍了基于杂交瘤技术制备小分子化合物单克隆抗体的方法，分析了影响杂交瘤技术制备小分子化合物单克隆抗体的因素，然后综述了小分子化合物单克隆抗体在兽药残留、药物开发、环境保护等动物医学领域的应用，最后展望了小分子化合物单克隆抗体生产和应用的发展趋势，为小分子化合物单克隆抗体的制备与应用相关研究提供指导。

关键词: 小分子化合物; 痕量检测; 单克隆抗体; 影响因素; 兽医临床

Abstract: The trace detection of pesticides,veterinary drugs,heavy metals,biotoxins and other residual substances is of great significance in the fields of medical diagnosis,food safety and environmental monitoring.The immunoassays based on antigen-antibody specific recognition have advantages of strong specificity,high sensitivity and good stability,simple and quick operation,etc.,but how to obtain specific antibodies against small molecular compounds is the key to develop immunoassays.In this paper,the hybridoma technology for the preparation of monoclonal antibody of small molecular compounds was firstly introduced.Important factors affecting the production of monoclonal antibody of small molecular compounds in hybridoma technology was then briefly analyzed.The applications of monoclonal antibody in veterinary medicine,such as veterinary drug residues detection,drug development and environmental protection,were reviewed.Finally,the development trend of the monoclonal antibody production and application was prospected.This review provided guidance for the preparation and application of the monoclonal antibodies of small molecular compound.

Key words: small molecular compounds; trace detection; monoclonal antibody; influence factor; veterinary clinic

中图分类号:

杨松鑫, 冯建远, 郭旋, 韦柳源, 梁翼莹, 严昊, 张子仪, 陈海兰. 小分子化合物单克隆抗体的制备及其在动物医学领域的应用[J]. 中国畜牧兽医, 2021, 48(3): 1121-1131.

YANG Songxin, FENG Jianyuan, GUO Xuan, WEI Liuyuan, LIANG Yiying, YAN Hao, ZHANG Ziyi, CHEN Hailan. Preparation of Monoclonal Antibodies for Small Molecular Compounds and Their Applications in the Field of Veterinary Medicine[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1121-1131.

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