牛源多杀性巴氏杆菌Oma87基因的分子特征、抗原性及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2020.07.025

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (7): 2200-2206.doi: 10.16431/j.cnki.1671-7236.2020.07.025

牛源多杀性巴氏杆菌Oma87基因的分子特征、抗原性及遗传进化分析

张星星¹, 黄新¹, 何延华¹, 何立雄², 韩猛立¹, 张倩¹, 吴桐忠¹, 钟发刚¹

1. 新疆农垦科学院畜牧兽医研究所, 省部共建绵羊遗传改良与健康养殖国家重点实验室, 石河子 832000;
2. 新疆兵团第四师畜牧兽医工作站, 可克达拉 835000

收稿日期:2020-02-04 出版日期:2020-07-20 发布日期:2020-07-18
通讯作者: 吴桐忠(1970-),男,新疆石河子人,助理研究员,研究方向:动物传染病诊断与防治,E-mail:1619683757@qq.com;钟发刚(1971-),男,新疆奇台人,研究员,研究方向:动物传染病诊断与防治,E-mail:zfg125@sohu.com E-mail:1619683757@qq.com;zfg125@sohu.com
作者简介:张星星(1990-),男,甘肃天水人,硕士,助理研究员,研究方向:动物传染病诊断与防治,E-mail:shzuzx@163.com
基金资助:
省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项项目（SKLSGIHP2017A03）；兵团国际科技合作计划项目（2019BC004）；兵团区域创新引导计划项目（2017BA038）；兵团重点领域科技攻关项目（2019AB029）

Molecular Characteristics,Antigenicity and Phylogenetic Analysis of Oma87 Gene of Pasteurella multocida in Cattle

ZHANG Xingxing¹, HUANG Xin¹, HE Yanhua¹, HE Lixiong², HAN Mengli¹, ZHANG Qian¹, WU Tongzhong¹, ZHONG Fagang¹

1. State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Institute of Animal Husbabdry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China;
2. Animal Husbandry and Veterinary Station of Xinjiang 4th Production and Construction Division, Kekedala 835000, China

Received:2020-02-04 Online:2020-07-20 Published:2020-07-18

摘要/Abstract

摘要： 为研究牛源多杀性巴氏杆菌（Pasteurella multocida，Pm）Oma87基因的分子特征及同源性，本研究对荚膜血清A型（capsular serotype A，Pm8）Oma87基因进行扩增、克隆和测序，利用在线软件对其进行分子特征、抗原性及遗传进化分析。结果显示，Oma87基因全长为2 376 bp，编码791个氨基酸；氨基酸序列分析发现，该蛋白为酸性的亲水性蛋白，稳定性高，具有信号肽结构但不存在跨膜区域；二、三级结构分析发现，该蛋白为无规则卷曲及多段延伸链所形成的"N"字型三维立体结构。遗传进化分析显示，新疆分离株Pm8 Oma87基因与其他不同地域的牛源A型Pm和部分猪源A型Pm的同源性较近，与其他型Pm的同源性较远；通过VaxiJen软件分析发现，Oma87蛋白的保护性抗原的总体预测值为0.5478，高于正常阈值0.4，证明Oma87蛋白可作为免疫原性蛋白，为进一步研究Oma87蛋白奠定了理论基础。

关键词: 多杀性巴氏杆菌; Oma87基因; 分子特征; 抗原性; 遗传进化

Abstract: In order to reveal the molecular characteristics and phylogenetic analysis of Oma87 gene of Pasteurella multocida (Pm),the Oma87 gene in Pm8 (capsular serotype A) was amplified and cloned,the sequence and antigenicity was analyzed by bioinformatics software and conducted to construct phylogenetic tree in this study.The results showed that the length of the Oma87 gene was 2 376 bp,which encoded 791 amino acids.Amino acid sequence analysis found that the Oma87 protein was an acidic protein with hydrophilicity,which had high stability.It had a signal peptide but without transmembrane domain.The secondary and tertiary structure analysis found that the Oma87 protein was "N" type three-dimensional structure formed by random coils connected multiple extended strands.The nucleotide homology analysis result showed that the Oma87 gene of Xinjiang isolated strain Pm8 was highly homologous with bovine Pm (capsular serogroup A) isolated and part of swine Pm (capsular serogroup A) from other different geographic regions,but not highly homologous to other capsular serotype group.VaxiJen classified Oma87 protein as probable antigen because it had a value of 0.5478 which was higher than the normal threshold value of 0.4,proved that Oma87 protein could act as the immunogenic protein,which laid a foundation for further study on Oma87 protein as Pm vaccine candidate antigen.

Key words: Pasteurella multocida; Oma87 gene; molecular characteristics; antigenicity; phylogenetic

中图分类号:

Q786

张星星, 黄新, 何延华, 何立雄, 韩猛立, 张倩, 吴桐忠, 钟发刚. 牛源多杀性巴氏杆菌Oma87基因的分子特征、抗原性及遗传进化分析[J]. 中国畜牧兽医, 2020, 47(7): 2200-2206.

ZHANG Xingxing, HUANG Xin, HE Yanhua, HE Lixiong, HAN Mengli, ZHANG Qian, WU Tongzhong, ZHONG Fagang. Molecular Characteristics,Antigenicity and Phylogenetic Analysis of Oma87 Gene of Pasteurella multocida in Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2200-2206.

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牛源多杀性巴氏杆菌Oma87基因的分子特征、抗原性及遗传进化分析

Molecular Characteristics,Antigenicity and Phylogenetic Analysis of Oma87 Gene of Pasteurella multocida in Cattle

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