鸭胸肌肉色性状全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2020.05.005

摘要/Abstract

摘要： 试验旨在利用全基因组关联分析（GWAS）定位影响鸭胸肌肉色性状的候选基因及分子标记，探究肉色性状的遗传基础。本研究中，共测定了555只北京鸭×野鸭F2代资源群体的3种胸肌肉色性状（包括红度a^*、黄度b^*、亮度L^*）。利用北京鸭×野鸭F2代资源群体胸肌肉色性状数据并结合全基因组重测序数据进行全基因组关联分析，检测影响胸肌肉色性状的相关基因及可能的因果变异位点。结果显示，肌肉亮度L^*、红度a^*、黄度b^*均属于低遗传力性状，遗传力分别为0.23、0.12、0.13，且肌肉黄度b^*变异系数较大（26.18%）。通过相关性分析可知，肌肉黄度与红度存在较强正表型相关（r=0.52），肌纤维直径与肌肉黄度存在弱负相关性（r=-0.16）。利用混合线性模型进行GWAS，发现了1个SNP与肌肉黄度b^*潜在显著关联（-log₁₀ P=8.28）。对最高效应SNP进行连锁不平衡检验，发现42个SNPs与最高点SNP存在高相关性（r²>0.4），这些SNPs位于9号染色体0.37~0.43 Mb之间，区间中共包含7个基因。通过转录组测序数据分析，发现只有5个基因在胸肌组织中表达。对这5个基因进行功能注释，确定硒蛋白T（SELENOT）基因为影响肌肉黄度的候选基因。该结果为解析鸭胸肌肉色性状和提高鸭肉品质的遗传改良提供重要参考。

关键词: 肉色; 全基因组关联分析; SELENOT基因; 鸭

Abstract: Genome-wide association analysis (GWAS) was used to identify candidate genes and molecular markers which genes related to meat color traits in ducks and explored the genetic basis of meat color traits.In this study,three meat color phenotypes (including redness a^*,yellowness b^* and brightness L^*) were collected in 555 ducks from an F2 segregating population generated by Pekin duck×mallard crosses and performed GWAS using whole-genome resequencing data to detect SNPs loci associated with meat color traits.The results showed that the meat color L^*,a^* and b^* displayed low heritability which was 0.23,0.12 and 0.13,respectively,and the coefficient of variation for meat color yellowness (b^*) was 26.18%.Correlation analysis results showed that high positive phenotypic and genetic correlations between muscle yellowness and muscle redness (r=0.52),and low negative correlations between muscle fiber diameter and muscle yellowness (r=-0.16).GWAS was performed using a mixed linear model and potential significant SNP association with muscle yellowness (-log₁₀ P=8.28) was found.Then the loci of interest more closely was examined by calculating pairwise linkage disequilibrium (LD).42 SNPs spanning a region from 0.37 Mb to 0.43 Mb on chromosome 9 where contained 7 annotated genes were highly correlated (pairwise r²>0.4).The mRNA expression of five genes were expressed in breast muscle.By functional annotation of this 5 genes,SELENOT was identified as candidate gene for yellowness b^*,which provided an important reference for the genetic improvement of meat quality in ducks.

Key words: meat color; GWAS; SELENOT gene; duck

中图分类号:

刘大鹏, 凡文磊, 唐静, 周正奎, 侯水生. 鸭胸肌肉色性状全基因组关联分析[J]. 中国畜牧兽医, 2020, 47(5): 1326-1333.

LIU Dapeng, FAN Wenlei, TANG Jing, ZHOU Zhengkui, HOU Shuisheng. Genome-wide Association Studies on Meat Color Traits in Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1326-1333.

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