2016—2017年广西牛呼吸道疾病综合征病原学的调查研究

doi:10.16431/j.cnki.1671-7236.2018.12.027

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (12): 3535-3544.doi: 10.16431/j.cnki.1671-7236.2018.12.027

2016—2017年广西牛呼吸道疾病综合征病原学的调查研究

吴翠兰^1,2, 彭昊^1,2, 李军^1,2, 李常挺^1,2, 陶立^1,2, 王秋华³, 潘艳^1,2, 韩林梅³, 陈泽祥^1,2, 曾芸³

1. 广西兽医研究所, 南宁 530001;
2. 广西兽医生物技术重点实验室, 南宁 530001;
3. 广西大学动物科学技术学院, 南宁 530005

收稿日期:2018-06-12 出版日期:2018-12-20 发布日期:2018-12-21
通讯作者: 李军, 曾芸 E-mail:jlee9981@163.com;yzeng323@163.com
作者简介:吴翠兰(1988-),女,湖南凤凰人,硕士,实习研究员,研究方向:动物疫病防控与病原分子生物学,E-mail:cuilanwu@163.com
基金资助:
广西科技重大专项（桂科AA17204057）；广西重点研发计划（桂科AB16380106）；国家现代农业产业体系广西肉牛肉羊产业创新团队建设（nycytxgxcxtd-09-05）；广西兽医生物技术重点实验室开放基金（16-380-45-B-2）

Research on Etiology of Bovine Respiratory Disease Complex in Guangxi During 2016 to 2017

WU Cuilan^1,2, PENG Hao^1,2, LI Jun^1,2, LI Changting^1,2, TAO Li^1,2, WANG Qiuhua³, PAN Yan^1,2, HAN Linmei³, CHEN Zexiang^1,2, ZENG Yun³

1. Guangxi Veterinary Research Institute, Nanning 530001, China;
2. Guangxi Key Laboratory of Veterinary Biotechnology, Nanning 530001, China;
3. College of Animal Science and Technology, Guangxi University, Nanning 530005, China

Received:2018-06-12 Online:2018-12-20 Published:2018-12-21

摘要/Abstract

摘要：

为了解广西地区牛呼吸道疾病综合征（bovine respiratory disease complex，BRDC）的病原情况，本研究通过RT-PCR/PCR方法和细菌分离鉴定方法对2016-2017年送检的117份患有呼吸道疾病的病料进行病原诊断，并对主要分离菌进行药物敏感性试验。结果显示，RT-PCR/PCR方法检测的肺炎克雷伯氏菌、牛支原体、化脓隐秘杆菌、多杀性巴氏杆菌、牛传染性鼻气管炎病毒（IBRV）、溶血性曼氏杆菌的检出率分别为41.0%（48/117）、28.2%（33/117）、20.5%（24/117）、15.4%（18/117）、12.8%（15/117）、5.1%（6/117），而细菌分离鉴定方法检测的肺炎克雷伯氏菌、大肠杆菌、牛支原体、化脓隐秘杆菌、多杀性巴氏杆菌、溶血性曼氏杆菌的检出率分别为41.0%（48/117）、33.3%（39/117）、17.1%（20/117）、7.7%（9/117）、2.6%（3/117）、2.6%（3/117），牛副流感病毒3型、牛呼吸道合胞体病毒和牛病毒性腹泻病毒未检出，且PCR方法更敏感。药敏试验结果显示，20株牛支原体对β-内酰胺类、磺胺类、多肽类药物的耐药率为90.0%~100.0%，对喹诺酮类、庆大霉素、阿米卡星和卡那霉素敏感（耐药率为在10.0%~25.0%）；30株大肠杆菌和30株肺炎克雷伯氏菌除了对头孢噻肟、头孢曲松和头孢他啶敏感外（耐药率分别为13.3%~20.0%、10.0%~30.0%），对其他15种药物均具有耐药性（耐药率分别为50.0%~100.0%、40.0%~100.0%）。综上所述，广西地区牛呼吸道疾病主要以牛支原体、大肠杆菌和肺炎克雷伯氏菌引发的混合感染为主，且分离菌均已出现不同程度的耐药性。

关键词: 牛呼吸道疾病综合征(BRDC); 病原学调查; 广西; 耐药性分析

Abstract:

To understand the etiology situation of bovine respiratory disease complex (BRDC) in Guangxi,117 samples with respiratory diseases during 2016 to 2017 were diagnosed by RT-PCR/PCR method and bacterial isolation and identification method.And the antibiotic susceptibility test of the main isolates were carried out.The results showed that detection rates of Klebsiella pneumoniae,Mycoplasma bovis,Trueperella pyogenes,Pasteurella multocida,IBRV and Mannheimia haemolytica were 41.0%(48/117),28.2%(33/117),20.5%(24/117),15.4%(18/117),12.8%(15/117) and 5.1%(6/117) detected by RT-PCR/PCR method,while the detection rates of Klebsiella pneumoniae,Escherichia coli,Mycoplasma bovis,Trueperella pyogenes,Pasteurella multocida and Mannheimia haemolytica by bactorial isolation and identification method were 41.0%(48/117),33.3%(39/117),17.1%(20/117),7.7%(9/117),2.6%(3/117) and 2.6%(3/117),indicating the former had the better sensibility,and BPIV3,BRSV and BVDV had not been detected.The results of antibiotic susceptibility tests showed that resistance rates of 20 Mycoplasma bovis strains against β-lactams,sulfonamides and peptides were from 90.0% to 100.0%,and they were sensitive to quinolones,gentamicin,amikacin and kanamycin (resistance rates were 10.0% to 25.0%).30 Escherichia coli strains and 30 Klebsiella pneumoniae strains were sensitive to cefotaxime,ceftriaxone and ceftazidime (13.3% to 20.0%,10.0% to 30.0%,respectively),and they were resistant to other 15 kinds of drugs (50.0% to 100.0%,40.0% to 100.0%,respectively).The results indicated that mixed infection was dominant in bovine respiratory disease in Guangxi caused by Mycoplasma bovis,Escherichia coli and Klebsiella pneumoniae which had developed different degrees of drug resistance.

Key words: bovine respiratory disease complex (BRDC); etiology investigation; Guangxi; drug resistance analysis

中图分类号:

S852.65

吴翠兰, 彭昊, 李军, 李常挺, 陶立, 王秋华, 潘艳, 韩林梅, 陈泽祥, 曾芸. 2016—2017年广西牛呼吸道疾病综合征病原学的调查研究[J]. 《中国畜牧兽医》, 2018, 45(12): 3535-3544.

WU Cuilan, PENG Hao, LI Jun, LI Changting, TAO Li, WANG Qiuhua, PAN Yan, HAN Linmei, CHEN Zexiang, ZENG Yun. Research on Etiology of Bovine Respiratory Disease Complex in Guangxi During 2016 to 2017[J]. , 2018, 45(12): 3535-3544.

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2016—2017年广西牛呼吸道疾病综合征病原学的调查研究

Research on Etiology of Bovine Respiratory Disease Complex in Guangxi During 2016 to 2017

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