Nupr1 mRNA在小鼠早期妊娠子宫中的表达

doi:10.16431/j.cnki.1671-7236.2018.10.033

摘要/Abstract

摘要：

试验旨在研究核蛋白1（nuclear protein 1，Nupr1）mRNA在小鼠早期妊娠子宫中的表达，探讨Nupr1与小鼠胚胎着床的相关性。通过建立小鼠早期妊娠模型、假孕妊娠模型、延迟着床及激活模型、人工蜕膜化模型和激素处理模型，采用原位杂交的方法检测Nupr1 mRNA在小鼠各种模型子宫组织中的定位表达情况，并应用实时荧光定量PCR法检测早期妊娠模型和假孕妊娠模型中Nupr1 mRNA的相对表达量。结果显示，Nupr1 mRNA在小鼠早期妊娠第1~4天子宫的腔上皮和腺上皮表达，第5~8天表达于蜕膜区域；假孕妊娠第1~5天，Nupr1 mRNA主要表达于小鼠子宫腔上皮和腺上皮；延迟着床模型中信号表达于在小鼠子宫的腔上皮和腺上皮，与正常妊娠第4天结果相似；延迟激活模型中信号表达于蜕膜区，与早期妊娠第5天表达结果相似；人工蜕膜化模型中信号表达于蜕膜区，而蜕膜对照组中信号表达于腔上皮和腺上皮；17β-雌二醇（oestrogen，E₂）处理组信号表达于腔上皮和腺上皮，信号增强，孕酮（progesterone，P₄）和E₂共同处理表达无明显变化；实时荧光定量PCR结果显示，正常妊娠第2天Nupr1 mRNA相对表达量较高，假孕妊娠第2天Nupr1 mRNA相对表达量也较高。本研究结果表明，Nupr1 mRNA在小鼠子宫中的表达与小鼠早期妊娠过程相关，Nupr1 mRNA在腔上皮和腺上皮的表达可能受激素调节，在子宫基质中的表达与蜕膜化及活化胚泡相关。

关键词: 核蛋白1(Nupr1); 胚胎着床; 原位杂交; 早期妊娠

Abstract:

The test was aimed to study the expression of nuclear protein 1 (Nupr1) mRNA in mouse uterus during early pregnancy.The method of in situ hybridization was used to investigate Nupr1 mRNA expression in animal models that included early pregnancy,pseudopregnancy,delayed implantation and activation,artificial decidualization and hormonal treatments.The relative expression level of Nupr1 mRNA was detected in early pregnancy and pseudopregnancy using Real-time PCR.During mouse early pregnancy,the signal of Nupr1 mRNA was detected in luminal epithelium and glandular epithelium during the 1st to 4th day and in the decidua area during the 5th to 8th day.Nupr1 mRNA was mainly expressed in the luminal epithelium and glandular epithelium of mose uterus on the 1st to 5th day of pseudopregnancy.The signal was detected in luminal epithelium and glandular epithelium of the mouse uterus in the delayed implantation,which was similar to the results of early pregnancy on the 4th day.The signal was detected in decidua in the model of delayed activation,which was similar to the results of early pregnancy on the 5th day.The expression of Nupr1 mRNA in the model of artificial decidualization was detected in decidua area.In the control of artificial decidualization the slight signal appeared in luminal epithelium and glandular epithelium of the mouse uterus.After treated with oestrogen (E₂) the signal appeared in luminal epithelium and glandular epithelium of the mouse uterus,and the signal was enhanced.After treated with both of E₂ and progesterone (P₄), the expression of the signal was not changed significantly.Real-time PCR result showed that the relative expression on the 2nd day was higher than other days in early pregnancy and pseudopregnancy.The results indicated that the expression of Nupr1 mRNA in mouse uterus was related to the process of mouse early pregnancy.The expression of signal in luminal epithelium and glandular epithelium of the mouse uterus might be regulated by hormones.Nupr1 mRNA expression in uterine stroma was associated with decidualization and active blastocysts.

Key words: Nupr1; embryo implantation; in situ hybridization; early pregnancy

中图分类号:

S857.2

刘珍珍, 王秋吉, 董昕玥, 杨启鑫, 单春华. Nupr1 mRNA在小鼠早期妊娠子宫中的表达[J]. 《中国畜牧兽医》, 2018, 45(10): 2943-2950.

LIU Zhenzhen, WANG Qiuji, DONG Xinyue, YANG Qixin, SHAN Chunhua. Expression of Nupr1 mRNA in Mouse Uterus During Early Pregnancy[J]. , 2018, 45(10): 2943-2950.

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