季节对新疆牛源E.coli O157:H7分布的影响研究

doi:10.16431/j.cnki.1671-7236.2018.01.027

摘要/Abstract

摘要：

试验旨在研究季节对牛源E.coli O157：H7分布的影响。在新疆地区A、B、C 3个牛场中，分别于春、夏、秋、冬4个季节采集牛肛拭子（399份）、粪便（68份）、水（29份）和饲料（43份）样本，先用EC肉汤增菌，再用山梨醇麦康凯培养基（SMAC）与4-甲基伞形酮-β-D葡萄糖醛酸苷（MUG）选择性培养，然后进行PCR鉴定和毒力基因检测。结果显示，从3个牛场539份样本中共检出E.coli O157：H7菌株5株（0.93%，5/539），其中春季样本检出2株（1.44%，2/139），秋季样本检出1株（0.56%，1/180），冬季样本检出2株（1.38%，2/145），未能从夏季样本中检出目标菌；B牛场的菌株检自肛拭子样本（0.69%，1/145），C牛场的菌株分离于饲料（20.00%，3/15）和肛拭子（0.66%，1/152）样本，水中未分离到目标菌。牛源E.coli O157：H7的分布具有明显的季节性，B牛场只在秋季的肛拭子样本中检出了1株E.coli O157：H7，C牛场分别在春季和冬季各检出2株，春、冬季E.coli O157：H7分离率高于夏、秋季。因此，在新疆特殊的气候特点和饲养模式下，防控牛源E.coli O157：H7的传播时，要十分注意寒冷季节圈舍内卫生的管理，尤其要严防饲料被粪便污染。

关键词: 牛; 季节; 大肠杆菌O157:H7; 分离鉴定

Abstract:

To study the impact of season to the distribution of bovine E.coli O157:H7,samples of anus swabs (399),feces (68),water (29) and feed (43) were collected in the spring, summer,autumn and winter from A,B and C farms of Xinjiang. After enrichment by EC broth, SMAC and MUG selective culture were then performed. Finally,PCR was used for identification and virulence gene detection of isolated strains. A total of 5 E.coli O157:H7 strains were isolated from 539 samples from three farms (0.93%,5/539), 2 of them were from spring (1.44%,2/139),1 from autumn(0.56%,1/180),2 from winter (1.38%,2/145) and no strains were isolated from summer samples. One strain were isolated from anus swab samples in farm B (0.69%,1/145) and one were isolated from anus swabs (0.66%,1/152) and three strains were isolated from feed samples in farm C (20.00%,3/15),and no target strains were isolated from water samples. The distribution of bovine E. coli O157:H7 had obvious seasonal characteristics.One E.coli O157:H7 strain of farm B was isolated from autumn and four of farm C were from isolated spring (2 strain) and winter (2 strain),and the isolation rate of E. coli O157:H7 in spring and winter were higher than that in summer and autumn. In conclusion,under the special climate characteristics and feeding mode in Xinjiang,to prevent and control the spreading of E. coli O157:H7 of cattle,we must pay great attention on hygiene management of pens at cold season, specially avoiding the feed contaminated by feces.

Key words: bovine; season; E.coli O157:H7; isolation and identification

中图分类号:

S852.61

苏战强, 王栋, 张瑾瑜, 张毅, 刘英玉, 马凯琪, 高姣姣, 孙雪, 姚刚. 季节对新疆牛源E.coli O157:H7分布的影响研究[J]. 《中国畜牧兽医》, 2018, 45(1): 212-218.

SU Zhanqiang, WANG Dong, ZHANG Jinyu, ZHANG Yi, LIU Yingyu, MA Kaiqi, GAO Jiaojiao, SUN Xue, YAO Gang. Study on Seasonal Impact to the Distribution of Bovine E.coli O157: H7 in Xinjiang[J]. , 2018, 45(1): 212-218.

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