不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响

doi:10.16431/j.cnki.1671-7236.2017.12.029

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (12): 3598-3604.doi: 10.16431/j.cnki.1671-7236.2017.12.029

不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响

陈晓兰¹, 杨海峰¹, 瞿静雯¹, 李冉¹, 蒋春茂¹, 陆广富¹, 伯若楠²

1. 江苏农牧科技职业学院, 泰州 225300;
2. 南京农业大学动物医学院, 南京 210095

收稿日期:2017-04-05 出版日期:2017-12-20 发布日期:2017-12-20
通讯作者: 蒋春茂 E-mail:cmj109@126.com
作者简介:陈晓兰(1979-),女,江苏海安人,博士,副教授,研究方向:中兽药药理及新药研发,E-mail:cxl7972563@163.com
基金资助:
国家自然科学基金项目（31702286）；江苏省产学研合作项目（BY2015066-01）；江苏省自然科学基金项目（BK20161368）；江苏省高校自然基金项目（16KJB230005）

Effect of Different Mulberry Leaf Extracts on Spleen Lymphocyte Proliferation in Mice

CHEN Xiao-lan¹, YANG Hai-feng¹, QU Jing-wen¹, LI Ran¹, JIANG Chun-mao¹, LU Guang-fu¹, BO Ruo-nan²

1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received:2017-04-05 Online:2017-12-20 Published:2017-12-20

摘要/Abstract

摘要：

试验旨在比较不同桑叶提取物对小鼠脾脏淋巴细胞增殖作用的影响。用不同浓度乙醇醇沉桑叶水提物，制备桑叶粗多糖MLP-1、MLP-2、MLP-3、MLP-4、MLP-5，采用苯酚硫酸法测定各提取物多糖含量。以5种桑叶粗多糖和桑叶水提物（MLAE）为试验药物，采用MTT法比较多糖含量分别在15.625、31.25、62.5、125、250 μg/mL浓度时各提取物单独刺激及协同LPS、PHA共同刺激小鼠脾脏B、T淋巴细增殖的变化。结果显示，多糖浓度在15.625~250 μg/mL范围内，各提取物无论单独还是协同LPS和PHA时均能促进小鼠脾脏B、T淋巴细胞的增殖，其中，MLP-1多糖含量在低浓度时能显著刺激脾脏B淋巴细胞增殖（P<0.05）；MLP-3和MLP-5在低浓度时表现出较强的刺激T淋巴细胞增殖的能力；MLAE协同LPS在大部分浓度点的脾脏B淋巴细胞的增殖能力均显著高于细胞对照组和LPS对照组，可显著提升体液免疫功能（P<0.05）。

关键词: 桑叶多糖; 桑叶水提物; 脾脏淋巴细胞; 增殖; 小鼠

Abstract:

This experiment was aimed to compare the effects of different mulberry leaf extract on spleen lymphocyte proliferation in mice. Mulberry leaf polysaccharides (MLP-1, MLP-2, MLP-3, MLP-4 and MLP-5) were prepared with mulberry leaf aqueous extract (MLAE) by different concentration of alcohol deposit, polysaccharides concentration of all extracts were determined by phenol sulfuric acid method. Five different MLPs and MLAE were as the experimental drug, when polysaccharide concentration was 15.625, 31.25, 62.5, 125 and 250 μg/mL, the changes of spleen B and T lymphocytes proliferation in mice which were stimulated by MLPs and MLAE in single or synergistical stimulation of drugs with LPS and PHA were determined by MTT method. The results showed that when polysaccharide concentration was in the range of 15.625 to 250 μg/mL, MLPs and MLAE in single or synergistical stimulation with LPS and PHA could stimulate the spleen B and T lymphocytes proliferation, MLP-1 at low concentration could stimulate significantly spleen B lymphocyte proliferation remarkably (P<0.05), and MLP-3 and MLP-5 at low concentrations showed strong stimulation ability of T lymphocyte proliferation. When MLAE was in synergistical stimulation with LPS at most concentrations points, the spleen B lymphocytes proliferation in mice were significantly higher than those of the cell control and LPS control groups, which revealed that MLAE could significantly improve the humoral immune function (P<0.05).

Key words: mulberry leaf polysaccharides (MLP); mulberry leaf aqueous extract (MLAE); splenic lymphocytes; proliferation; mice

中图分类号:

S852.2

陈晓兰, 杨海峰, 瞿静雯, 李冉, 蒋春茂, 陆广富, 伯若楠. 不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响[J]. 《中国畜牧兽医》, 2017, 44(12): 3598-3604.

CHEN Xiao-lan, YANG Hai-feng, QU Jing-wen, LI Ran, JIANG Chun-mao, LU Guang-fu, BO Ruo-nan. Effect of Different Mulberry Leaf Extracts on Spleen Lymphocyte Proliferation in Mice[J]. , 2017, 44(12): 3598-3604.

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不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响

Effect of Different Mulberry Leaf Extracts on Spleen Lymphocyte Proliferation in Mice

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