灌胃不同奶样对BALB/c小鼠致敏性的影响

doi:10.16431/j.cnki.1671-7236.2017.07.041

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 2191-2196.doi: 10.16431/j.cnki.1671-7236.2017.07.041

灌胃不同奶样对BALB/c小鼠致敏性的影响

白娜¹, 明亮¹, 高婉婷¹, 郭富城¹, 乔向宇¹, 朝格苏力德¹, 郭雁¹, 伊日贵¹, 刘东辉¹, 吉日木图^1,2

1. 内蒙古农业大学, 乳品生物技术与工程教育部重点实验室, 呼和浩特 010018;
2. 内蒙古骆驼研究院, 阿拉善盟 737300

收稿日期:2017-01-12 出版日期:2017-07-20 发布日期:2017-07-22
通讯作者: 吉日木图 E-mail:yeluotuo1999@vip.163.com
作者简介:白娜(1991-),女,内蒙古鄂尔多斯人,硕士生,研究方向:乳品生物技术,E-mail:baina0929@163.com
基金资助:
国家自然科学基金项目（31360397）；国际科技合作项目（2015DFR30680、ky201401022）；内蒙古自治区重大专项

Effects of Different Milk Samples on Sensitization in BALB/c Mice

BAI Na¹, MING Liang¹, GAO Wan-ting¹, GUO Fu-cheng¹, QIAO Xiang-yu¹, CHAOGE Sulide¹, GUO Yan¹, YI Ri-gui¹, LIU Dong-hui¹, JI Rimutu^1,2

1. Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Camel Research Institute of Inner Mongolia, Alex 737300, China

Received:2017-01-12 Online:2017-07-20 Published:2017-07-22

摘要/Abstract

摘要：

本试验研究了驼奶、牛奶和人奶对BALB/c小鼠的致敏性强弱，将60只小鼠随机分为5组，每组12只，分别为β-乳球蛋白（β-lg）组（阳性对照组）、牛奶组、驼奶组、人奶组和空白组（阴性对照组），每组分别灌胃1 mg/g体重的样品和0.3 μg/g体重的霍乱弧菌毒素（CT），空白组灌胃PBS和CT，每周1次。灌胃6周后，通过观察过敏症状，检测血清特异性免疫球蛋白E（IgE）和IgG1、血浆组胺水平及血管通透性等指标，比较几种奶源的致敏性强弱。结果显示，驼奶组、人奶组和空白组小鼠体重正常增长，而β-lg组和牛奶组的体重增长有减慢的趋势。与空白组相比，β-lg组和牛奶组的血清特异性IgE和IgG1水平极显著升高（P<0.01），组胺水平极显著升高（P<0.01），血管通透性增加，过敏症状明显；而驼奶组小鼠血清IgE和IgG1水平极显著低于牛奶组（P<0.01），但与人奶组相比无显著差异（P>0.05），过敏症状轻微，表明驼奶的致敏性明显低于牛奶，且与人奶相似。

关键词: BALB/c小鼠; 驼奶; 牛奶; 过敏; β -乳球蛋白

Abstract:

In order to study the allergenicity of camel milk,cow milk and human milk,sixty BALB/c mice were randomly divided into β-lactoglobulin (β-lg) group (positive control group),cow milk group,camel milk group,human milk group and blank group (negative control group). Each group of mice received 1 mg/g BW samples and 0.3 μg/g cholera toxin (CT) per week,while mice in control group were received PBS and CT. After six weeks of intragastric administration,some allergic symptoms,the serum specific immunoglobulin E (IgE) and immunoglobulin G1 (IgG1) levels,plasma histamine levels and vascular permeability were detected. The results showed that the body weight of mice in camel milk,human milk and blank groups were normally increased,while that in β-lg and cow milk groups tended to slow down. The serum-specific IgE,IgG1 levels and histamine level in β-lg and cow milk groups were extremely significantly higher than blank group (P<0.01),the vascular permeability was increased,and the allergy symptoms were obvious. While the specific IgE and IgG1 levels of camel milk group were extremely significantly lower than cow milk group (P<0.01),but there were no significant difference with human milk group (P>0.05),and the allergy symptoms were mild. This study showed that the sensitization of camel milk was lower than cow milk,and similar to human milk.

Key words: BALB/c mice; camel milk; cow milk; allergy; β-lg

中图分类号:

S852.2

白娜, 明亮, 高婉婷, 郭富城, 乔向宇, 朝格苏力德, 郭雁, 伊日贵, 刘东辉, 吉日木图. 灌胃不同奶样对BALB/c小鼠致敏性的影响[J]. 《中国畜牧兽医》, 2017, 44(7): 2191-2196.

BAI Na, MING Liang, GAO Wan-ting, GUO Fu-cheng, QIAO Xiang-yu, CHAOGE Sulide, GUO Yan, YI Ri-gui, LIU Dong-hui, JI Rimutu. Effects of Different Milk Samples on Sensitization in BALB/c Mice[J]. , 2017, 44(7): 2191-2196.

参考文献

[1] 李冰,龙再浩.牛奶蛋白过敏及其改性研究[J].农产品加工,2012,6:41-44.
[2] Li X M,Schofield B H,Huang C K,et al. A murine model of IgE mediated cow milk hypersensitivity[J]. Journal of Allergy & Clinical Immunology,1999,103(1):206-214.
[3] 陆东林,张静,何晓瑞.驼奶的化学成分和加工利用[J].奶品加工,2008,7:36-38.
[4] Zhang H,Yao J,Zhao D,et al. Changes in chemical composition of Alxa bactrian camel milk during lactation[J].Dairy Sci,2005,88(10):3402-3410.
[5] Frossard C P, Hauser C,Eigenmann P A. Antigen-specific secretory IgA antibodies in the gut are decreased in a mouse model of food allergy[J]. Journal of Allergy & Clinical Immunology,2004,114(2):377-382.
[6] 段翠翠.不同奶源蛋白致敏性的评估[D].哈尔滨:东北农业大学,2014.
[7] Gonipeta B, Parvataneni S,Tempelman R J,et al. An adjuvant-free mouse model to evaluate the allergenicity of milk whey protein[J]. Journal of Dairy Science,2009,92(10):4738-4744.
[8] 孙明芳,江米足.婴儿牛奶蛋白过敏与胃食管反流关系的研究进展[J].中国当代儿科杂志,2016,18(7):666-670.
[9] Wershil B K, Furuta G T,Wang Z S,et al. Mast cell-dependent neutrophil and mononuclear cell recruitment in immunoglobulin E-induced gastric reactions in mice[J].Gastroenterology,1996,110(5):1482-1490.
[10] Fiocchi A,Dahdah L,Albarini M,et al. Cow's milk allergy in children and adults[J].Chemical Immunology & Allergy,2015,101:114-123.
[11] 王宏. BALB/c小鼠过敏反应模型的建立及在中药注射剂致敏性检测中的应用[D].济南:山东大学,2012.
[12] Gueders M M, Paulissen G,Crahay C,et al. Mouse models of asthma:A comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness,inflammation,and cytokine production[J]. Inflammation Research,2009,58(12):845-854.
[13] 刘俊红,吴斌,赖乾坤,等.SD幼鼠食物过敏模型的建立与评价[J].临床儿科杂志,2013,31(10):953-958.
[14] 朱庆龄,李锋,王俊丽,等.经口食物过敏大鼠动物模型的建立[J].中国当代儿科杂志,2016,18(8):757-761.
[15] Ishizaka K,Ishizaka T,Hornbrook M M. Physico-chemical properties of human reaginic antibody.Ⅳ. Presence of a unique immunoglobulin as a carrier of reaginic activity[J].Journal of Immunology,1966,97(1):75-85.
[16] Ishizaka K,Ishizaka T,Hornbrook M M. Allergen-binding activity of gamma-E,gamma-G and gamma-A antibodies in sera from atopic patients. In vitro measurements of reaginic antibody[J].Journal of Immunology,1967,98(98):490-501.
[17] Jr B A,Brooks J R,Sampson H A. Allergenicity of major component proteins of soybean determined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting in children with atopic dermatitis and positive soy challenges[J]. Journal of Allergy & Clinical Immunology,1988,81(6):1135-1142.
[18] 陈晨.转基因食品致敏性评价BALB/c小鼠动物模型和RBL-2H3细胞模型研究[D].北京:中国疾病预防控制中心,2013.
[19] 李中港.过敏反应动物模型的建立及在双黄连注射剂过敏反应检测中的应用[D].济南:山东大学,2010.
[20] Shabo Y,Barzel R,Margoulis M,et al.Camel milk for food allergies in children[J].Israel Medical Association Journal Imaj,2006,7(12):796-798.
[21] El-Agamy E I,Nawar M,Shamsia S M, et al. Are camel milk proteins convenient to the nutrition of cow milk allergic children[J].Small Ruminant Research,2009,82(1):1-6.
[22] Ehlayel M S,Hazeima K A,Al-Mesaifri F,et al. Camel milk:An alternative for cow's milk allergy in children[J].Allergy & Asthma Proceedings,2011,32(3):255-258.

灌胃不同奶样对BALB/c小鼠致敏性的影响

Effects of Different Milk Samples on Sensitization in BALB/c Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	唐庆凤, 李玲, 黄加祥, 方文远, 农皓如, 唐艳, 陆呈委, 韦盘秋, 冯玲. 广西地区不同饲养模式及不同季节水牛奶霉菌毒素污染状况调查[J]. 中国畜牧兽医, 2022, 49(9): 3665-3675.
[2]	范琳琳, 李慧颖, 姚倩倩, 杨怀谷, 王加启, 郑楠, 文芳. 牛奶中活性蛋白和活性脂肪酸生物活性研究进展[J]. 中国畜牧兽医, 2021, 48(1): 395-405.
[3]	杨晋辉, 王孟辉, 曹静, 胡鹏丽, 李洪亮, 高飞, 钱文涛. 放牧对乳成分和乳产品感官品质影响的研究进展[J]. 中国畜牧兽医, 2020, 47(10): 3193-3202.
[4]	罗润博, 张养东, 郑楠, 王加启. 奶及奶制品中兽药残留高通量检测方法研究进展[J]. 中国畜牧兽医, 2020, 47(10): 3410-3416.
[5]	齐云霞, 赵小伟, 黄冬维, 蔡东东, 杨永新, 程广龙, 赵辉玲. 超速离心和酸沉淀制备牛乳清的蛋白质组学研究[J]. 《中国畜牧兽医》, 2019, 46(8): 2203-2210.
[6]	梁钰, 张坤, 王梦云, 赵新豪, 李春丽. 猪β防御素研究进展[J]. 《中国畜牧兽医》, 2019, 46(8): 2346-2353.
[7]	罗铮, 翁吉梅, 艾锦新, 龙安炬, 罗卫星, 蔡惠芬. 黔北麻羊Tβ4基因多态性及生物信息学分析[J]. 《中国畜牧兽医》, 2019, 46(8): 2406-2414.
[8]	王勇, 韩雨奇, 卢明华. β-受体激动剂药物残留检测方法研究进展[J]. 《中国畜牧兽医》, 2019, 46(8): 2478-2487.
[9]	刘昆, 毛伟, 李婷婷, 刘博, 关红, 裴乐, 曹金山. 大肠杆菌与金黄色葡萄球菌对奶牛子宫内膜组织细胞因子表达及损伤程度的影响[J]. 《中国畜牧兽医》, 2019, 46(6): 1841-1848.
[10]	解津刚, 巴合提·博代, 热衣扎别克·哈德坎, 吾热力哈孜·哈孜汗. 哈萨克羊INHβA基因克隆及生物信息学分析[J]. 《中国畜牧兽医》, 2019, 46(5): 1290-1298.
[11]	王俊书, 徐进强, 金红岩, 侯绍华, 封家旺. 兔奇异变形杆菌的分离鉴定及耐药性分析[J]. 《中国畜牧兽医》, 2019, 46(5): 1508-1515.
[12]	范一萍, 王晓梅, 贾启涛, 李成勃, 梁小娟, 曹春伟, 周磊, 陶聪, 赵建国, 刘剑锋, 王彦芳. 猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析[J]. 《中国畜牧兽医》, 2019, 46(3): 774-781.
[13]	许丽文, 王秋玲, 王芳芳, 刘诚刚, 马得莹. 人工感染鸭源新城疫病毒对绍鸭β-防御素和细胞因子基因表达的影响[J]. 《中国畜牧兽医》, 2019, 46(2): 536-547.
[14]	温贵兰, 张升波, 李昌红, 徐丽, 田浪, 文明, 王开功, 程振涛, 赵德刚. 从江香猪IFN-β基因的序列分析及原核表达[J]. 《中国畜牧兽医》, 2019, 46(1): 10-19.
[15]	谭斌, 杨胜林, 杨汝才, 王旭平, 周美迪, 周璇, 杨世皓. SMAD3基因在畜牧生产中的应用研究进展[J]. 《中国畜牧兽医》, 2019, 46(1): 185-193.