贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究

doi:10.16431/j.cnki.1671-7236.2016.12.032

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (12): 3300-3305.doi: 10.16431/j.cnki.1671-7236.2016.12.032

贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究

黄勋和¹, 张金枫¹, 陈洁波¹, 叶伟庆², 李威娜¹, 钟福生¹, 杜炳旺²

1. 嘉应学院生命科学学院, 梅州 514015;
2. 广东海洋大学农学院, 湛江 524088

收稿日期:2016-04-13 出版日期:2016-12-20 发布日期:2016-12-22
通讯作者: 杜炳旺 E-mail:dudu903@163.com
作者简介:黄勋和(1982-),男,广东河源人,博士,讲师,研究方向:中国家鸡遗传多样性与进化,E-mail:hxh826@jyu.edu.cn
基金资助:
广东省自然科学基金项目（2014A030307018）；嘉应学院“创新强校工程”项目（CQX019）；广东省科技计划项目（2015A020208020、2016A030303068）；国家科技基础条件平台-特种动物子平台建设子课题（201520）；嘉应学院重点科技计划项目（2015KJM03）

Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken

HUANG Xun-he¹, ZHANG Jin-feng¹, CHEN Jie-bo¹, YE Wei-qing², LI Wei-na¹, ZHONG Fu-sheng¹, DU Bing-wang²

1. School of Life Sciences, Jiaying University, Meizhou 514015, China;
2. College of Agricultural, Guangdong Ocean University, Zhanjiang 524088, China

Received:2016-04-13 Online:2016-12-20 Published:2016-12-22

摘要/Abstract

摘要：

为研究贵妃鸡的遗传多样性，试验应用主要组织相容性复合体B区域（MHC-B）复合微卫星位点LEI0258测定了贵妃鸡的等位基因信息，结合已发表的序列，进行等位基因与核苷酸多态性、单倍型中介网络图分析。40份样品中共检测到9个等位基因，长度为193~297 bp，其中5个为贵妃鸡特有。观察杂合度（Ho）、期望杂合度（He）和多态信息含量（PIC）分别为0.625、0.716和0.666。3个等位基因与6种已知MHC血清型相匹配。基于侧翼区变异信息的中介网络图将等位基因分为5个进化枝，贵妃鸡9个等位基因分布于A和B进化枝中，这2个进化枝同样存在于红原鸡中。研究结果表明贵妃鸡保持着中等偏上的遗传多样性水平，可能起源于红原鸡。

关键词: 贵妃鸡; 多态性; 主要组织相容性复合体; 微卫星位点LEI0258; 进化; 血清型

Abstract:

The aim of this study was to evaluate the polymorphism and evolution in royal chicken using the tandem repeat microsatellite LEI0258 located within the chicken major histocompatibility complex B region (MHC-B). Genomic DNA was extracted from forty blood samples of royal chicken and used to be genotyped and sequenced. Combining the published sequences,the analysis of allele and nucleotide polymorphism and median-joining network were carried out. A total of nine alleles ranging from 193 to 297 bp in 40 samples were found,five of which was novel for royal chicken. The observed heterozygosity,expected heterozygosity and polymorphic information content were 0.625,0.716 and 0.666,respectively. Three alleles were corresponding to six available serotypes. Alleles were classified into five clusters,based on the SNPs and indels found within the sequences flanking the repeats. The alleles of royal chicken existed in clades A and B,both of which were also found in Red junglefowl. The results suggested that royal chicken had a middle or high level of genetic diversity in microsatellite LEI0258,and that might originate from Red junglefowl.

Key words: royal chicken; polymorphism; major histocompatibility complex; microsatellite LEI0258; evolution; serotype

中图分类号:

S831.2

黄勋和, 张金枫, 陈洁波, 叶伟庆, 李威娜, 钟福生, 杜炳旺. 贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究[J]. 《中国畜牧兽医》, 2016, 43(12): 3300-3305.

HUANG Xun-he, ZHANG Jin-feng, CHEN Jie-bo, YE Wei-qing, LI Wei-na, ZHONG Fu-sheng, DU Bing-wang. Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken[J]. , 2016, 43(12): 3300-3305.

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贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究

Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken

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