蛹虫草多糖对注射环磷酰胺小鼠抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2016.12.025

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (12): 3251-3256.doi: 10.16431/j.cnki.1671-7236.2016.12.025

蛹虫草多糖对注射环磷酰胺小鼠抗氧化功能的影响

王米, 王宵旸, 张丽芳, 费陈忠, 张可煜, 王春梅, 杨锐乐, 薛飞群

中国农业科学院上海兽医研究所, 动物药学研究室, 上海 200241

收稿日期:2016-02-25 出版日期:2016-12-20 发布日期:2016-12-22
通讯作者: 薛飞群 E-mail:feiqun@gmail.com
作者简介:王米(1975-),男,四川绵阳人,博士,副研究员,研究方向:中兽药,E-mail:wangmi@shvri.ac.cn
基金资助:
公益性行业（农业）科研专项（201303040-08）；863计划课题“偶蹄动物用新型免疫佐剂的创新”（2011AA2114）。

The Effect of Cordyceps Militaris Polysaccharides on the Antioxidant Function in Mice Injected With Cyclophosphamide

WANG Mi, WANG Xiao-yang, ZHANG Li-fang, FEI Chen-zhong, ZHANG Ke-yu, WANG Chun-mei, YANG Rui-le, XUE Fei-qun

Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

Received:2016-02-25 Online:2016-12-20 Published:2016-12-22

摘要/Abstract

摘要：

试验旨在阐明蛹虫草多糖（CMP）对小鼠抗氧化活性的影响。选取90只雄性BALB/c小鼠，体重18~20 g，随机分为6组，3个CMP组（低、中、高CMP组），模型对照组（CY组）、阳性对照组（PC组）及空白对照组（BC组）各1个。CMP组和CY组分别腹腔注射80 mg/kg环磷酰胺，连续3 d，然后3个CMP组分别灌胃给予17.5、35.0、70.0 mg/kg体重的CMP，BC组和CY组给予生理盐水，PC组灌胃给予70 mg/kg体重的CMP，持续18 d。于最后一次给药24 h后，采集小鼠内脏，测定心脏、肝脏及肾脏匀浆液中T-AOC、MAD、CAT、SOD和GSH-Px水平。结果显示，与CY组相比，3个CMP处理组小鼠内脏匀浆液中的SOD和T-AOC活性均极显著增加（P<0.01），MDA水平极显著降低（P<0.01）；低剂量CMP组心脏CAT无显著变化（P>0.05），中、高剂量CMP组心脏CAT水平分别显著（P<0.05）、极显著（P<0.01）提高；除低剂量CMP组肝脏GSH-Px活性显著增加（P<0.05）外，其余CMP组内脏GSH-Px活性均极显著增加（P<0.01）。本试验中CMP可以有效提高小鼠心脏、肝脏及肾脏的抗氧化活性，这为抗氧化新药的研发提供了借鉴。

关键词: 蛹虫草多糖; 环磷酰胺; 抗氧化活性; 小鼠

Abstract:

To investigate the antioxidant effects of the Cordyceps militaris polysaccharides(CMP),90 male BALB/c mice were randomly divided into six groups, three experimental groups (CMP groups), model control group (CY group), blank control group (BC group) and positive control group (PC group). The mice in CMP and CY groups were given cyclophosphamide at 80 mg/kg via intraperitoneal injection for 3 d. Then the CMP groups were administrated 17.5, 35.0 and 70.0 mg/(kg·BW) CMP via gavage, respectively, BC and CY groups were given physiological saline, and PC group was orally administered 70 mg/(kg·BW) CMP, lasting 18 d. At 24 h after the last administration, heart, liver and kidney were collected to measure the levels of MAD, CAT, SOD, GSH-Px and T-AOC in the homogenate.SOD and TAOC activity of three CMP groups increased significantly (P<0.01), and the level of MDA decreased significantly (P<0.01) compared with CY group. As for CAT activity of heart, the value in low-dose CMP group had no significant change (P>0.05), while in middle and high-dose groups had significant (P<0.05) and extremely significant(P<0.01) increase respectively. Except that the hepatic GSH-Px activity increased significantly (P<0.05) in low-dose CMP group, the rest values of GSH-Px activities in all CMP groups increased extremely significantly (P<0.01). The results indicated that CMP could effectively improve the antioxidant function of mice and had the potential to be a good resource of new antioxidant drugs.

Key words: Cordyceps militaris polysaccharides; cyclophosphamide; antioxidant activity; mice

中图分类号:

S853.7

王米, 王宵旸, 张丽芳, 费陈忠, 张可煜, 王春梅, 杨锐乐, 薛飞群. 蛹虫草多糖对注射环磷酰胺小鼠抗氧化功能的影响[J]. 《中国畜牧兽医》, 2016, 43(12): 3251-3256.

WANG Mi, WANG Xiao-yang, ZHANG Li-fang, FEI Chen-zhong, ZHANG Ke-yu, WANG Chun-mei, YANG Rui-le, XUE Fei-qun. The Effect of Cordyceps Militaris Polysaccharides on the Antioxidant Function in Mice Injected With Cyclophosphamide[J]. , 2016, 43(12): 3251-3256.

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蛹虫草多糖对注射环磷酰胺小鼠抗氧化功能的影响

The Effect of Cordyceps Militaris Polysaccharides on the Antioxidant Function in Mice Injected With Cyclophosphamide

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