牛奶和山羊奶中乳脂球膜蛋白的比较研究

doi:10.16431/j.cnki.1671-7236.2016.11.023

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 2963-2969.doi: 10.16431/j.cnki.1671-7236.2016.11.023

牛奶和山羊奶中乳脂球膜蛋白的比较研究

赵小伟, 杨永新, 黄冬维, 程广龙, 赵辉玲

安徽省农业科学院畜牧兽医研究所, 合肥 230031

收稿日期:2016-03-21 出版日期:2016-11-20 发布日期:2016-11-18
通讯作者: 杨永新 E-mail:yyongxin@yahoo.com
作者简介:赵小伟(1985-),男,安徽蚌埠人,硕士,助理研究员,研究方向:动物分子营养,E-mail:xiaowei1986mm@163.com
基金资助:
公益性行业（农业）科研专项课题“生鲜乳质量特征评价技术研究”（201403071-3）；牛奶品质调控及乳房炎防控技术创新团队项目（14C0403）

Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat

ZHAO Xiao-wei, YANG Yong-xin, HUANG Dong-wei, CHENG Guang-long, ZHAO Hui-ling

Institute of Animal Sciences and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received:2016-03-21 Online:2016-11-20 Published:2016-11-18

摘要/Abstract

摘要：

试验旨在分析牛奶与山羊奶中乳脂肪球膜（milk fat globule membrane，MFGM）蛋白组成及潜在的生物学功能。采集牛奶和山羊奶各30份，离心分离牛奶和山羊奶中脂肪，提取MFGM蛋白，液相色谱串联质谱分析结合数据库搜索鉴定，比较了奶牛和山羊MFGM蛋白的差异，分析了MFGM蛋白参与的生物过程、分子功能及相关代谢通路。结果显示，奶牛和山羊MFGM中分别鉴定了284和334个蛋白，奶牛和山羊中蛋白参与的生物学过程的模式非常相似，主要涉及生物调控、定位、转运、信号转导和应激反应等。分子功能方面二者存在一定的差异，奶牛MFGM蛋白主要涉及蛋白结合和核苷酸结合，而山羊MFGM蛋白主要涉及蛋白结合和结构分子活性。奶牛和山羊中差异表达的MFGM蛋白主要涉及结构分子活性。通路分析发现，奶牛和山羊MFGM蛋白涉及的生物学通路稍有差异，其中紧密接头、轴突导向、抗原加工与递呈、补体和凝血级联反应通路在奶牛MFGM蛋白中得到富集，而调节肌动蛋白细胞骨架通路在山羊奶中得到富集。研究结果展示了奶牛和山羊MFGM蛋白的表达模式及差异，为进一步探索两种奶畜乳腺合成脂肪球的分子机制的异同提供科学依据。

关键词: 乳脂球膜蛋白; 液相串联质谱; 牛奶; 山羊奶

Abstract:

In order to compare the difference of milk fat globule membrane（MFGM）proteins between dairy cow and goat milk,30 milk samples were collected in a dairy farm and a goat farm from Anhui area,respectively.Extracted proteins from the MFGM-enriched fractions were identified and quantified by LC/MS approach.The results showed that 284 and 334 proteins of MFGM from dairy cow and goat were identified,the biological processes of MFGM proteins were similar between dairy cow and goat which were mainly related to biological regulation,localization,transport,signal transduction and response to stimulus.Meanwhile,there were some differences in molecular functions,and protein binding and nucleotide binding were the most prevalent molecular functions in dairy cow MFGM proteins,while protein binding and structural molecule activity were the most prevalent molecular functions in goat MFGM proteins.And structural molecule activity was the main molecular functions among the difference proteins.Pathway analysis revealed that tight junction,axon guidance,antigen processing and presentation,complement and coagulation cascades were enrichment in dairy cow MFGM proteins,and regulation of actin cytoskeleton was enrichment in goat MFGM proteins.Those results revealed the protein expression pattern difference between MFGM protein of dairy cow and goat milk,and provide reference data for further exploring the molecular mechanism of synthetic milk fat globule.

Key words: milk fat globule membrane; LC/MS; dairy cow milk; goat milk

中图分类号:

TS252.2

赵小伟, 杨永新, 黄冬维, 程广龙, 赵辉玲. 牛奶和山羊奶中乳脂球膜蛋白的比较研究[J]. 《中国畜牧兽医》, 2016, 43(11): 2963-2969.

ZHAO Xiao-wei, YANG Yong-xin, HUANG Dong-wei, CHENG Guang-long, ZHAO Hui-ling. Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat[J]. , 2016, 43(11): 2963-2969.

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牛奶和山羊奶中乳脂球膜蛋白的比较研究

Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat

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