奶牛分子育种技术研究进展

doi:10.16431/j.cnki.1671-7236.2016.10.027

摘要/Abstract

摘要：

分子育种技术是在DNA水平上直接进行选择，通过对随机分布于整个基因组的分子标记的多态性进行比较，更加全面地了解研究对象的多态性，可以获得更大的信息量，选种的准确性也更高，进而提高奶牛生产效率和经济效益。作者从奶牛分子育种技术的角度入手，阐述了数量性状基因的定位、分子标记辅助选择、转基因育种以及全基因组关联分析这几方面的研究难点、存在问题和研究进展，指出分子育种技术在奶牛育种及改良过程中具有非常重要的作用，进一步研究并应用分子育种技术，不仅可以加速我国奶牛核心群的全面建设，而且对培育种公牛也是非常必要的手段。

关键词: 奶牛; 分子育种技术; 研究进展

Abstract:

Molecular breeding technology is direct selection at the DNA level by randomly distributed molecular markers throughout the genome polymorphism comparison,it is helpful to carry out more comprehensive study of polymorphism on investigated subjects,meanwhile,more information can be got,and the accuracy of selection will be higher,thereby,the milk production efficiency and economic benefits can be improved.From the perspective of the dairy cattle molecular breeding technology,the author expounds the research challenges,problems and research progress on several aspects,such as localization of QTL,MAS,transgenic breeding and GWAS,points out that molecular breeding techniques have a very important role in dairy cattle breeding and improvement process.Further research and application of molecular breeding technology not only can accelerate the development of dairy cows in the core group of comprehensive construction,but also is very necessary to cultivate a bull.

Key words: dairy cattle; molecular breeding technology; research progress

中图分类号:

S813.3

蒋秋斐, 罗晓瑜, 洪龙, 温万, 田佳, 张娟, 李欣, 韩丽云, 史远刚, 顾亚玲. 奶牛分子育种技术研究进展[J]. 《中国畜牧兽医》, 2016, 43(10): 2694-2700.

JIANG Qiu-fei, LUO Xiao-yu, HONG Long, WEN Wan, TIAN Jia, ZHANG Juan, Li Xin, HAN Li-yun, SHI Yuan-gang, GU Ya-ling. The Research Progress of Molecular Technology for Dairy Cattle Breeding[J]. , 2016, 43(10): 2694-2700.

参考文献

[1] 白文林,尹荣焕,尹荣兰,等.动物分子育种的研究与实践[M].辽宁:辽宁科学技术出版社,2013.
[2] Liu Y,Chen Q,Zhang J T.Tumor suppressor gene 14-3-3 sigma is down-regulated whereas the protooncogene translation elongation factor 1 delta is up-regulated in non-small cell lung cancers as identified by proteomic profiling[J].J Proteome Res,2004,3(4):728-735.
[3] Liu Z,Cordes J.DNA marker technologies and their applications in aquaculture genetics[J].Aquaculture,2004,238(1):1-37.
[4] 金钟城.分子数量遗传学和动物分子育种[J].草食家畜,1997,1:6-10.
[5] 郭亚宁,许尚忠,杨公社,等.畜禽基因图谱研究进展[J].家畜生态,2004,25(4):166-171.
[6] 王勇强,张沅,张勤.家畜数量性状基因定位研究进展[J].中国畜牧杂志,2000,36(1):41-43.
[7] 马玉萍,潘晓亮,赵宗胜,等.畜禽QTL定位研究[J].畜牧兽医杂志,2003,22(6):17-19.
[8] 美国农业部国家基因组研究计划网站.http://www.animalgenome.org/cgi-bin/QTLdb/BT/index[OL].2016.
[9] 徐华.中国荷斯坦奶牛催乳素基因和微卫星DNA多态性与产奶性能的相关分析[D].保定:河北农业大学,2004.
[10] 刘延鑫,李大齐,崔群维,等.奶牛HSP70基因表达及其连锁微卫星标记与耐热性状的相关性[J].遗传,2010,32(9):61-64.
[11] Honaramooz A,Megee S,Zeng W,et al.Adeno-associated virus (AAV)-mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation[J].FASEB J,2008,22(2):374-382.
[12] 钟晓琳.荷斯坦奶牛氮代谢差异及相关微卫星标记的研究[D].郑州:河南农业大学,2014.
[13] Vignal A,Milan D,San Cristobal M,et al.A review of molecular markers on SNP and other types and their use in animal genetics[J].Genet Sel Evol,2002,34(3):275-305.
[14] Meuwissen T H E,van Arendonk J A M.Potential improvements in rate of genetic gain from marker-assisted selection in dairy cattle breeding schemes[J].J Dairy Sci,1992,75(6):1651-1659.
[15] 张润锋,陈宏,蓝贤勇,等.西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析[J].畜牧兽医学报,2005,36(6):545-549.
[16] Pan C Y,Lan X Y,Chen H,et al.A DdeI PCR-RFLP detecting a novel missense mutation of POU1F1 gene showed no effects on growth traits in cattle[J]. Czech J Anim Sci,2008,53(12):523-527.
[17] 严林俊,刘波,房兴堂,等.秦川牛和中国荷斯坦牛POU1F1基因多态性研究[J].遗传,2006,28(11):1371-1375.
[18] LU A,Hu X C,Chen H,et al.Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins[J].Mol Biol Rep,2010,37(1):547-551.
[19] 刘新武,昝林森,张佳兰.中国荷斯坦奶牛Leptin基因第2外显子多态性及其与产奶性状的相关性[J].西北农林科技大学学报(自然科学版),2008,36(11):7-9.
[20] 杨东英,陈宏,张良志.中国5个牛种Leptin基因外显子3的多态性分析[J].西北农林科技大学学报(自然科学版),2006,34(8):17-20.
[21] 鲍斌,房兴堂,陈宏,等.中国荷斯坦牛STAT5A基因遗传多态性与泌乳性状的相关分析[J].中国农业科学,2008,41(6):1872-1878.
[22] 张润锋,陈宏,雷初朝,等.西安地区荷斯坦奶牛群5个基因位点遗传多态性与泌乳性状的相关分析[J].农业生物技术学报,2006,14(3):329-333.
[23] Zhang C L,Chen H,Wang Y H,et al.Association of a missence mutation in serotonin receptor 1B (HTR1B) gene with milk production traits[J].Research in Veterinary Science,2008,85:265-268.
[24] 昝林森,张佳兰,刘新武,等.牛AGPAT6基因遗传特征与奶牛产奶性能相关性研究[J].中国农业科学,2007,40(7):1498-1503.
[25] 李春苗,石万海,储明星,等.荷斯坦母牛TLR1基因多态性及其与体细胞评分关系[J].中国农业科学,2009,42(6):2118-2125.
[26] 赵中利. MBL2基因与奶牛乳腺炎抗性研究[D].长春:吉林大学,2012.
[27] 陈丹霞,陈宏,张春雷,等.奶牛分子育种研究进展[J].中国牛业科学,2009,35(6):41-45.
[28] Yang D Y,Chen H,Zhang Z F,et al.Association of polymorphisms of Leptin gene with body weight and body sizes indexes in Chinese indigenous cattle[J].Journal of Genetics and Genomics,2007,34(5):400-405.
[29] 张润锋,陈宏,雷初朝,等.IGFBP3基因PCR-RFLP多态性与中国荷斯坦牛泌乳性状的相关分析[J].中国畜牧杂志,2006,42(3):9-11.
[30] 程维杰,李秋玲,王长法,等.荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系[J].遗传,2009,31(2):169-174.
[31] 王洪梅,孔振兴,王长法,等.奶牛乳铁蛋白基因5'侧翼区遗传多态性及其与乳腺炎关联性分析[J].遗传,2009,31(4):393-399.
[32] 雷雪芹,陈宏,袁志发,等.牛FSHR基因第10外显子单核苷酸多态性及其与双胎性状的关系[J].中国生物化学与分子生物学报,2004,20(1):34-37.
[33] 刘鹏渊,朱军.标记辅助选择改良数量性状的研究进展[J].遗传,2001,23(4):375-380.
[34] 黄晨,李思易.分子标记辅助选择技术的应用研究[J].生命科学仪器,2008,6(12):47-50.
[35] 刘根娣,刘晓毅,薛文通.乳铁蛋白在动植物中的表达及应用[J].西北民族大学学报,2005,26(3):49-52.
[36] 罗庆苗,苗向阳,张瑞杰.转基因动物新技术研究进展[J].遗传,2011,33(5):449-458.
[37] Brophy B,Smolenski G,Wheeler T,et al.Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein[J].Nat Biotecknol,2003,21(2):157-162.
[38] Wall R J,Powell A M,Paape M J,et al.Genetically enhanced cows resist intramammary staphylococcus aureus infection[J].Nat Biotechnol,2005,23(4):445-451.
[39] Ebert K M,Selgrath J P,Ditullio P,et al.Transgenic production of a variant of human tissue type plasminogen activator in goat milk;generation of transgenic goats and analysis of expression[J].Biotechnology (NY),1991,9(9):835-838.
[40] Yu S,Luo J,Song Z,et al.Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle[J].Cell Research,2011,21:1638-1640.
[41] Baldassarre H,Duncan K,Hockley M,et al.Lactation performance of transgenic goats expressing recombinant human butyryl-cbolinesterase in the milk[J]. Transgenic Res,2011,20(6):1265-1272.
[42] 苟克勉,安晓荣,田见晖,等.动物乳腺生物反应器的现状和趋势[J].生物工程学报,2002,18(2):144-148.
[43] Polejaeva L A,Chen S H,Vanght T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells[J].Nature,2000,407:86.
[44] 施振旦,李万利,张永亮,等.动物转基因技术研究新进展[J].农业生物技术学报,2008,16(1):163-168.
[45] Daetwyler H D,Schenkel F S,Sargolzaei M,et al.A genome scan to detect quantitative trait loci for economically important traits in Holstein cattle using two methods and a dense single nucleotide polymorphism map[J].J Dairy Sci,2008,91(8):3225-3236.
[46] Bastiaansen J W M,Bovenhuis H,Wijga S,et al.Genome-wide association study for milk production and fat to protein ratio in dairy cattle[J].Human Factors & Ergonomics Society Meeting,2010,58(1):77-81.
[47] Jiang L,Liu J,Sun D,et al.Genome wide association studies for milk production traits in Chinese Holstein population[J].PLoS One,2010,5(10):e13661.
[48] Meredith B K,Kearney F J,Finlay E K.Genome-wide associations for milk production and somatic cell score in Holstein-Friesian cattle in Ireland[J].BMC Genet,2012,13(3):21.
[49] Schopen G C,Visker M H,Koks P D,et al.Whole-genome association study for milk protein composition in dairy cattle[J]. J Dairy Sci,2011,94(6):3148-3158.
[50] Bouwman A C,Bovenhuis H,Visker M,et al.Genome-wide association of milk fatty acids in Dutch dairy cattle[J]. BMC Genet,2011,12(5):43.
[51] Cole J B,Wiggans G R,Ma L.Genome-wide association analysis of thirty one production,health,reproduction and body conformation traits in contemporary US Holstein cows[J].BMC Genomics.2011,12(3):408.
[52] Streit M,Wellmann R,Reinhardt F,et al.Using genome-wide association analysis to characterize environmental sensitivity of milk traits in dairy cattle[J].G3(Bethesda),2013,3(7):1085-1093.
[53] Raven L A,Cocks B G,Hayes B J.Multibreed genome wide association can improve precision of mapping causative variants underlying milk production in dairy cattle[J].BMC Genomics,2014,15:62.
[54] Buitenhuis B,Janss L,Poulsen N A,et al.Genome-wide association and biological pathway analysis for milk-fat composition in Danish Holstein and Danish Jersey cattle[J].BMC Genomics,2014,15(1):1-11.
[55] De Los Campos G,Hickey J M,Pong-Wong R,et al.Whole-genome regression and prediction methods applied to plant and animal breeding[J].Genetics,2013,193(2):327-345.
[56] Pearson T A,Manolio T A.How to interpret a genome-wide association study[J].JAMA,2008,299(11):1335-1344.
[57] Andersson L,Georges M.Domestic-animal genomics:Deciphering the genetics of complex traits[J].Nat Rev Genet,2004,5(3):202-212.
[58] 杨少华,中国荷斯坦牛TRIB3和SAA家族基因对产奶性状的遗传效应分析及功能验证[D].北京:中国农业大学,2015.