《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (10): 2655-2660.doi: 10.16431/j.cnki.1671-7236.2016.10.021

• 生理生化 • 上一篇    下一篇

不同月龄马身猪和大白猪血清生长激素及其受体的变化规律

石建中1, 刘艺2, 王泽艺2, 申楠2, 张宁芳2, 成志敏2, 高鹏飞2, 郭晓红2, 李步高2, 曹果清2   

  1. 1. 大同市种猪场, 大同 037000;
    2. 山西农业大学动物科技学院, 太谷 030801
  • 收稿日期:2016-06-14 出版日期:2016-10-20 发布日期:2016-10-28
  • 通讯作者: 曹果清 E-mail:anniecao710502@aliyun.com
  • 作者简介:石建中(1964-),男,山西大同人,硕士,高级兽医师,研究方向:养猪生产与管理,E-mail:zzcsjz@126.com;刘艺(1994-),女,山西运城人,学士,研究方向:动物科学,E-mail:1512615357@qq.com
  • 基金资助:

    山西省科技攻关项目(20140311020-5);山西省高等学校优秀青年学术带头人支持计划;山西农业大学“中青年学术带头人及学术骨干”支持计划;山西省大学生科技创新项目(13-004);山西省研究生教育创新项目(2015SY25)

Study on the Change Trend of Growth Hormone (GH) and Growth Hormone Receptor (GHR) in Serum at Different Months in Mashen and Large White Pigs

SHI Jian-zhong1, LIU Yi2, WANG Ze-yi2, SHEN Nan2, ZHANG Ning-fang2, CHENG Zhi-min2, GAO Peng-fei2, GUO Xiao-hong2, LI Bu-gao2, CAO Guo-qing2   

  1. 1. Datong Pig Breeding Farm, Datong 037000, China;
    2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
  • Received:2016-06-14 Online:2016-10-20 Published:2016-10-28

摘要:

试验旨在探讨马身猪和大白猪血清中生长激素(growth hormone,GH)及其受体(growth hormone receptor,GHR)在生长发育过程中的变化规律,采用ELISA方法检测马身猪和大白猪0~6月龄血清中GH和GHR的浓度。结果表明,马身猪和大白猪在0~6月龄血清中GH浓度变化趋势基本相似,随着日龄的增长,血清中GH浓度呈逐渐上升的趋势,分别在4和5月龄时达到最高值,随后又逐渐降低,但与峰值无显著差异(P>0.05),在3和4月龄时,马身猪血清中GH浓度略高于大白猪,而在其他月龄,大白猪血清中GH均高于马身猪;0~6月龄,马身猪血清中GHR浓度无显著差异(P>0.05),大白猪血清中GHR的含量在1月龄时最低,与4月龄差异极显著(P<0.01),与6月龄差异显著(P<0.05),其他月龄间差异不显著(P>0.05),0~2月龄,马身猪血清中GHR含量高于大白猪,但只有在1月龄时差异达极显著水平(P<0.01);3~6月龄,大白猪血清中GHR含量高于马身猪,且在4和6月龄时差异显著(P<0.05),5月龄时差异极显著(P<0.01)。血清中GH和GHR浓度与猪的发育阶段和遗传背景有关,其变化规律与猪生长速度的变化趋势相一致。

关键词: 猪; 生长激素; 生长激素受体; 变化规律; ELISA

Abstract:

The purpose of this study was to understand the change trend of growth hormone (GH) and growth hormone receptor (GHR) in serum of Mashen and Large White pigs during the period from 0 to 6 months of age,and to analyze the influence of GH and GHR on growth rate.The method of ELISA was used to detect the concentration of GH and GHR in serum of Mashen and Large White pigs from 0 to 6 months of age.The results showed that the variation trends of GH in Mashen pig was roughly the same as in Large White pig,the GH concentration was increased with age increasing after birth and reached the peaks at 4 and 5 months of age for Mashen and Large White pigs,respectively,and then decreased gradually.The serum GH concentration in Mashen pig was a little greater than that in Large White pig at 3 and 4 months of age,on the contrary,the serum GH concentration in Large White pig was greater than that in Mashen pig at other months.During the period from 0 to 6 months of age,the difference of GHR concentration in serum was not significant in Mashen pig (P>0.05).In Large White pig,the serum GHR concentration at 1 month of age was lowest,and was significantly lower than that at 4 and 6 months of age (P<0.01;P<0.05).During the period of 0 to 2 months of age,the GHR concentration in Mashen pig was greater than that in Large White pig,but the difference was extremely significant only at 1 month of age (P<0.01).Conversely,the serum GHR concentration in Large White pig was greater than those in Mashen pig during the period from 3 to 6 month of age,there was significant difference at 4 and 6 months of age (P<0.05),and there was extremely significant at 5 months of age(P<0.01).The concentration of GH and GHR in serum was related to the developmental stages and genetic background of pig,and its change trend was in accordance with the trend of growth rate.

Key words: pig; GH; GHR; change trend; ELISA

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