猪MAP3K5基因CDS序列克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.10.001

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (10): 2509-2517.doi: 10.16431/j.cnki.1671-7236.2016.10.001

• 生物技术 • 下一篇

猪MAP3K5基因CDS序列克隆及生物信息学分析

蒲蕾^1,2, 岳静伟¹, 张金山¹, 刘欣¹, 王立刚¹, 颜华¹, 侯欣华¹, 高红梅¹, 张恬¹, 张跃博¹, 武群清^1,3, 张龙超¹, 王立贤¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 天津农学院动物科学与动物医学学院, 天津 300384;
3. 湖南农业大学动物科学技术学院, 长沙 410128

收稿日期:2016-05-04 出版日期:2016-10-20 发布日期:2016-10-28
通讯作者: 张龙超, 王立贤 E-mail:zhlchias@163.com;iaswlx@263.net
作者简介:蒲蕾(1987-),女,陕西西安人,博士,研究方向:猪遗传育种,E-mail:pulei87@126.com
基金资助:
中国农业科学院科技创新工程（ASTIP-IAS02）；国家“十二五”科技支撑计划项目（2015BAD03B02-2）；国家现代农业产业技术体系（CARS-36）

Cloning and Bioinformatics Analysis of MAP3K5 Gene CDS Sequence in Pig

PU Lei^1,2, YUE Jing-wei¹, ZHANG Jin-shan¹, LIU Xin¹, WANG Li-gang¹, YAN Hua¹, HOU Xin-hua¹, GAO Hong-mei¹, ZHANG Tian¹, ZHANG Yue-bo¹, WU Qun-qing^1,3, ZHANG Long-chao¹, WANG Li-xian¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
3. College of Animal Science Technology, Hunan Agricultural University, Changsha 410128, China

Received:2016-05-04 Online:2016-10-20 Published:2016-10-28

摘要/Abstract

摘要：

试验克隆了猪MAP3K5基因cDNA序列，分析了猪MAP3K5基因与MAP3K家族及其他物种MAP3K5基因的序列同源性，并研究了其保守结合域。通过RACE-PCR扩增获得猪MAP3K5基因5 452 bp的序列，并分析其可能的开放阅读框及预测蛋白序列，获得了MAP3K5基因31个外显子结构。通过比对发现，猪MAP3K5基因与MAP3K家族其他成员的mRNA及蛋白质序列的同源性较低，均在50%以下，其中猪MAP3K5与MAP3K6基因序列的同源性相对较高；猪MAP3K5基因与其他物种的MAP3K5基因的mRNA及蛋白质序列的同源性较高，均在78%以上，其中猪MAP3K5基因与牛、绵羊、犬和人的MAP3K5基因序列的同源性较高。对与猪MAP3K5序列同源性更高的蛋白质进行保守结构域分析，发现MAP3K6与MAP3K5的保守域和结合位点类似，其他MAP3K家族成员与MAP3K5的结构域差别较大；而其他物种的MAP3K5与猪MAP3K5相比保守结构域和结合位点相似。结果初步表明了猪MAP3K5序列和结构域特点，可为后续MAP3K5基因的功能研究奠定基础。

关键词: 猪; MAP3K5基因; 序列比对; 结构域

Abstract:

In this study,we cloned MAP3K5 gene cDNA sequence,compared the sequence of MAP3K5 with MAP3K families in pig and other species,and studied their conservative structure domain.Obtained the full-length of MAP3K5 gene (5 452 bp) by RACE-PCR,predicted the open reading frame (ORF) and amino acid (AA) sequence,and obtained the information of 31 exons.The low similarity were found in mRNA and amino acid sequences of MAP3K families in pig (<50%).And MAP3K5 had the comparatively high similarity with MAP3K6 in pig.The comparative analyzed the mRNA and protein sequence of pig MAP3K5 gene with other species MAP3K5 gene,founded that the ten species of MAP3K5 mRNA and protein sequence had the high similarity (>78%),and pig MAP3K5 had the comparatively high similarity with Bos taurus,Ovis aries,Canis lupus and Homo sapiens.Function domain analyzed the high similarity protein with MAP3K5 in pig,this research found that pig MAP3K6 protein had the similar conserved function domain with pig MAP3K5.However,the other pig MAP3K families didn't have the similar conserved function domain with pig MAP3K5.The ten species of MAP3K5 protein had the similar conserved function domain.The results obtained the sequence and molecular structure of MAP3K5 gene,and provided an important foundation for further function research of MAP3K5 gene in pig.

Key words: pig; MAP3K5 gene; sequence alignment; function domain

中图分类号:

蒲蕾, 岳静伟, 张金山, 刘欣, 王立刚, 颜华, 侯欣华, 高红梅, 张恬, 张跃博, 武群清, 张龙超, 王立贤. 猪MAP3K5基因CDS序列克隆及生物信息学分析[J]. 《中国畜牧兽医》, 2016, 43(10): 2509-2517.

PU Lei, YUE Jing-wei, ZHANG Jin-shan, LIU Xin, WANG Li-gang, YAN Hua, HOU Xin-hua, GAO Hong-mei, ZHANG Tian, ZHANG Yue-bo, WU Qun-qing, ZHANG Long-chao, WANG Li-xian. Cloning and Bioinformatics Analysis of MAP3K5 Gene CDS Sequence in Pig[J]. , 2016, 43(10): 2509-2517.

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猪MAP3K5基因CDS序列克隆及生物信息学分析

Cloning and Bioinformatics Analysis of MAP3K5 Gene CDS Sequence in Pig

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