水貂阿留申病毒地方强毒株ADV-LN的分离鉴定

doi:10.16431/j.cnki.1671-7236.2016.06.030

›› 2016, Vol. 43 ›› Issue (6): 1597-1603.doi: 10.16431/j.cnki.1671-7236.2016.06.030

水貂阿留申病毒地方强毒株ADV-LN的分离鉴定

贾赟^1,2, 李艳伍^1,2, 孙铭英², 张雪², 张岩岩³, 宋大贺², 刘钊², 王全凯², 孙颖杰⁴

1. 吉林农业大学, 长春 130000;
2. 辽宁出入境检验检疫局, 大连 116000;
3. 辽宁省水产技术推广总站, 沈阳 116015;
4. 四川出入境检验检疫局, 成都 610000

收稿日期:2016-02-04 出版日期:2016-06-20 发布日期:2016-07-11
通讯作者: 贾赟, 孙颖杰 E-mail:jxy750921@163.com;sunyjciq@163.com
作者简介:贾赟(1975-),男,河南鹤壁人,博士,高级兽医师,研究方向:动物分子病毒学;李艳武(1977-)男,河南开封人,博士,高级兽医师,研究方向:动物传染病防制,E-mail:jxy750921@163.com
基金资助:
国家质检总局科研项目(2013IK036);"十二五"国家科技支撑计划项目(2013BAD12B01)

Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN

JIA Yun^1,2, LI Yan-wu^1,2, SUN Ming-ying², ZHANG Xue², ZHANG Yan-yan³, SONG Da-he², LIU Zhao², WANG Quan-kai², SUN Ying-jie⁴

1. Jilin Agricultural University, Changchun 130000, China;
2. Liaoning Entry Exit Inspection and Quarantine Bureau, Dalian 116000, China;
3. Liaoning Fisheries Technology Extension Station, Shenyang 116015, China;
4. Sichuan Entry Exit Inspection and Quarantine Bureau, Chengdu 610000, China

Received:2016-02-04 Online:2016-06-20 Published:2016-07-11

摘要/Abstract

摘要： 本研究采用对流免疫电泳方法(CIEP)检测辽宁水貂养殖场疑似水貂阿留申病毒(aleutian mink disease virus,ADV)水貂血清抗体,采集抗体阳性水貂的肝脏、脾脏、肾脏和肠系膜淋巴结组织,电镜观察存在细小病毒样颗粒。组织液研磨无菌处理后,接种CRFK细胞,盲传6代,取病毒细胞分离液用PCR方法检测,呈ADV阳性。将病毒分离液纯化后接种健康水貂,隔离观察,接种后3 d即出现食欲减退,贫血,被毛无光泽,后期出现拒食、狂饮、死亡,个别水貂出现神经症状,表现抽搐、痉挛、步态蹒跚、共济失调,证明分离获得的病毒为ADV强毒株,命名为ADV-LN株。

关键词: 水貂阿留申病毒; 分离; 鉴定

Abstract: Mink suspected infection aleutian mink disease virus (ADV) from mink breeding areas in Liaoning province were tested with CIEP method.The mink with antibody to ADV were selected and culled.Liver,spleen,kidney and mesenteric lymph node samples were taken for pathological examination and the viruses were observed under electron microscope.The grinded tissue fluid filter was added penicillin and treptomycin and inoculated into CRFK cells and passaged by 6 times for virus isolation.And cells cultures were identified as ADV by PCR.Then they were inoculated into healthy mink.Three days later,the mink showed clinical signs,which including the loss of appetite,anemia,hair dull,antifeedant and binge drinking.Some minks showed neurological symptoms,manifested symptoms of convulsions,cramps,staggering gait,ataxia,or hind limb paralysis and died.The virus strains isolated and identified were named as the ADV-LN.

Key words: aleutian mink disease virus; isolation; identification

中图分类号:

S852.65

贾赟, 李艳伍, 孙铭英, 张雪, 张岩岩, 宋大贺, 刘钊, 王全凯, 孙颖杰. 水貂阿留申病毒地方强毒株ADV-LN的分离鉴定[J]. , 2016, 43(6): 1597-1603.

JIA Yun, LI Yan-wu, SUN Ming-ying, ZHANG Xue, ZHANG Yan-yan, SONG Da-he, LIU Zhao, WANG Quan-kai, SUN Ying-jie. Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN[J]. , 2016, 43(6): 1597-1603.

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水貂阿留申病毒地方强毒株ADV-LN的分离鉴定

Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN

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