不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究

doi:10.16431/j.cnki.1671-7236.2016.04.005

›› 2016, Vol. 43 ›› Issue (4): 879-891.doi: 10.16431/j.cnki.1671-7236.2016.04.005

不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究

付雪峰¹, 杨涵羽璐^2,3, 石刚¹, 徐新明¹, 张恩平⁴, 狄江¹, 张艳花¹, 黄锡霞², 田可川¹

1. 新疆畜牧科学院, 新疆绒毛用羊遗传育种与繁殖实验室, 乌鲁木齐 830000;
2. 新疆农业大学, 乌鲁木齐 830052;
3. 石河子大学, 石河子 832003;
4. 西北农林科技大学, 杨凌 712100

收稿日期:2015-10-15 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 黄锡霞, 田可川 E-mail:au_huangxixia@163.com;tiankechuan@163.com
作者简介:付雪峰(1980-),男,河南漯河人,硕士,研究方向:动物遗传育种与繁殖,E-mail:fuxuefengfeng@126.com
基金资助:
国家绒毛用羊产业技术体系建设专项(CARS-40);农业科研杰出人才培养计划项目;新疆维吾尔自治区研究生科研创新项目(XJGRI2013110);家养动物种资质源平台项目

Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter

FU Xue-feng¹, YANG Han-yulu^2,3, SHI Gang¹, XU Xin-ming¹, ZHANG En-ping⁴, DI Jiang¹, ZHANG Yan-hua¹, HUANG Xi-xia², TIAN Ke-chuan¹

1. The Laboratory of Genetics, Breeding and Reproduction for Xinjiang Wool & Cashmere Sheep, Xinjiang Academy of Animal Sciences, Urumqi 830000, China;
2. Xinjiang Agricultural University, Urumqi 830052, China;
3. Shihezi University, Shihezi 832003, China;
4. North West Agriculture and Forestry University, Yangling 712100, China

Received:2015-10-15 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 试验旨在研究不同纤维直径的细毛羊皮肤组织中毛囊差异表达的蛋白质,探讨与羊毛细度相关的蛋白质功能。应用双向凝胶电泳技术建立细毛羊皮肤组织中毛囊差异表达蛋白质图谱;运用ImageMaster 2D Platinum软件检测细毛羊毛囊组织差异表达蛋白质;通过质谱鉴定技术和数据库比对等方法开展细毛羊皮肤组织毛囊特异性蛋白质分类和功能鉴定。结果成功鉴定出94个差异蛋白质,其中,相同年龄超细型细毛羊和细型细毛羊皮肤毛囊组织中有73个差异蛋白质;不同年龄的超细型细毛羊毛囊组织中有21个差异蛋白质。按功能属性划分得到了角蛋白、分子伴侣类蛋白、细胞骨架结构类蛋白、14-3-3蛋白、蛋白酶类、肌球蛋白、血清白蛋白和其他蛋白8类功能蛋白。结果提示,应用生物信息学技术分析这些差异蛋白与羊毛纤维直径的内在联系,为优质细毛羊的培育提供直接有效的基因资源信息,对提高羊毛品质具有重要的意义。

关键词: 细毛羊; 双向凝胶电泳; 蛋白质组学; 差异表达蛋白质

Abstract: The purpose of this paper was to study the differentially expressed proteins of hair follicles in skin tissue with different fiber diameter of Fine-wool sheep, and discussed the function of proteins related to wool fineness.The differentially expressed protein maps of Fine-wool sheep skin hair follicle were established by two-dimensional gel electrophoresis, and the differentially expressed protein spots were detected and analyzed by the ImageMaster 2D Platinum software, Fine-wool sheep skin hair follicle specific proteins were classified and functional identified by mass spectrum identification technology and database matching method.The results showed that 94 different proteins were successfully identified in Fine-wool sheep with different fiber diameter, including 73 different proteins between superfine wool sheep and Fine-wool sheep at the same age, and 21 different proteins of superfine wool sheep at the different age.According to the functional properties got eight kind of functional proteins, keratin, molecular chaperone protein, cytoskeleton protein, 14-3-3 protein, protease, myosin, serum albumin, other protein, respectively.These results indicated that the internal relation between the differentially expressed protein and the wool fiber diameter was analyzed by the bioinformatics knowledge, which would provide effective genetic resources information directly for high quality breeding of Fine-wool sheep, and had important practical significance for improving the quality of the wool.

Key words: Fine-wool sheep; two-dimensional gel electrophoresis; proteomics; differentially expressed proteins

中图分类号:

TQ93

付雪峰, 杨涵羽璐, 石刚, 徐新明, 张恩平, 狄江, 张艳花, 黄锡霞, 田可川. 不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究[J]. , 2016, 43(4): 879-891.

FU Xue-feng, YANG Han-yulu, SHI Gang, XU Xin-ming, ZHANG En-ping, DI Jiang, ZHANG Yan-hua, HUANG Xi-xia, TIAN Ke-chuan. Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter[J]. , 2016, 43(4): 879-891.

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不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究

Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter

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