北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析

doi:10.16431/j.cnki.1671-7236.2015.07.025

›› 2015, Vol. 42 ›› Issue (7): 1793-1799.doi: 10.16431/j.cnki.1671-7236.2015.07.025

北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析

梁晶¹, 王红卫², 程利安², 张龙超¹, 王立刚¹, 刘欣¹, 石国华², 杨洪平², 刘先策², 施辉毕¹, 张跃博¹, 张恬¹, 王立贤¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 北京黑六牧业科技有限公司, 北京 102211

收稿日期:2014-12-23 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 王红卫, 王立贤 E-mail:1127077916@qq.com;iaswlx@263.net
作者简介:梁晶(1989-),男,安徽淮北人,博士,研究方向:猪遗传育种,Tel:010-62816011;E-mail:jing_224@126.com
基金资助:
北京市科技计划(Z121100001512004);国家自然科学基金(31201781);中国农业科学院基本科研业务专项(2012ZL069、2014ZL006)

Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs

LIANG Jing¹, WANG Hong-wei², CHENG Li-an², ZHANG Long-chao¹, WANG Li-gang¹, LIU Xin¹, SHI Guo-hua², YANG Hong-ping², LIU Xian-ce², SHI Hui-bi¹, ZHANG Yue-bo¹, ZHANG Tian¹, WANG Li-xian¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Beijing Heiliu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China

Received:2014-12-23 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 试验旨在研究猪过氧化物酶体增殖物激活受体δ(PPARD)基因第1外显子上的错义突变位点G32E(g.61399G>A)多态性与北京黑猪脂肪沉积相关性状的关联。利用Sequenom质谱检测平台检测北京黑猪及多个中外猪种群体G32E位点的基因型,并分析该位点多态性与北京黑猪背膘厚及肌内脂肪的关联性。结果发现,在北京黑猪中,G32E位点GG基因型个体背膘厚性状极显著高于GA和AA基因型个体(P<0.01),与肌内脂肪含量的关联未达到显著水平(P>0.05)。在梅山猪和二花脸猪中,主要以A等位基因为主;而在中国地方猪种民猪、莱芜黑猪、丫杈猪及国外商品猪种杜洛克猪、长白猪和大白猪中,G等位基因频率高。初步认为PPARD基因G32E位点多态性对背膘厚性状有显著影响,对肌内脂肪含量性状没有影响。

关键词: 猪; PPARD基因; DNA多态性; 背膘厚

Abstract: The objective of this study was to analyze the association of a missense mutation G32E in the first exon of peroxisome proliferator-activated receptor delta (PPARD) gene with fat deposit related traits in Beijing Black pigs.Detected the genotype at G32E site in Beijing Black pig population and several Chinese and foreign pig populations using Sequenom mass spectrometry,and analyzed polymorphism with backfat thickness and IMF in Beijing Black pig population.The results indicated that backfat thickness of individuals with genotype GG was extremely significant higher than those individuals with genotypes GA and AA (P<0.01) in Beijing Black pig population,but no significant different of IMF was found (P>0.05).Allele A was high in Chinese local pigs Meishan and Erhualian.But in Minzhu,Laiwu,Yacha,and foreign commercial pigs Duroc,Landrace,Large White,mainly was allele G.The result suggested that polymorphism of PPARD gene G32E site had significant impact on backfat thickness,and no effect on IMF trait.

Key words: pig; PPARD gene; DNA polymorphism; backfat thickness

中图分类号:

S813.3

梁晶, 王红卫, 程利安, 张龙超, 王立刚, 刘欣, 石国华, 杨洪平, 刘先策, 施辉毕, 张跃博, 张恬, 王立贤. 北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析[J]. , 2015, 42(7): 1793-1799.

LIANG Jing, WANG Hong-wei, CHENG Li-an, ZHANG Long-chao, WANG Li-gang, LIU Xin, SHI Guo-hua, YANG Hong-ping, LIU Xian-ce, SHI Hui-bi, ZHANG Yue-bo, ZHANG Tian, WANG Li-xian. Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs[J]. , 2015, 42(7): 1793-1799.

参考文献

[1] Hoque M A,Suzuki K,Kadowaki H,et al.Genetic parameters for feed efficiency traits and their relationships with growth and carcass traits in Duroc pigs[J].J Anim Breed Genet,2007,124(3):108-116.
[2] 孙华,宋忠旭,李良华,等.中国大白猪SⅡ1系主要生长和胴体性状的遗传参数估测[J].湖北农业科学,2009,48:3086-3089.
[3] Evans R M,Barish G D,Wang Y X.PPARs and the complex journey to obesity[J].Nat Med,2004,10:355-361.
[4] Barak Y,Liao D,He W,et al.Effects of peroxisome proliferator-activated receptor delta on placentation,adiposity,and colorectal cancer[J].Proc Natl Acad Sci USA,2002,99(1):303-308.
[5] Huang W H,Ma Z X,Xu Z Y,et al.Detection of novel SNPs and mapping of the fatness QTL on pig chromosome 7q1.1-1.4 region[J].Genet Mol Res,2011,10:3090-3097.
[6] Rohrer G A.Identification of quantitative trait loci affecting birth characters and accumulation of backfat and weight in a Meishan-White composite resource population[J].J Anim Sci,2000,78:2547-2553.
[7] de Koning D J,Janss L L,Rattink A P,et al.Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa)[J].Genetics,1999,152:1679-1690.
[8] Sanchez M P,Riquet J,Iannuccelli N,et al.Effects of quantitative trait loci on chromosomes 1,2,4,and 7 on growth,carcass,and meat quality traits in backcross Meishan × Large White pigs[J].J Anim Sci,2006,84:526-537.
[9] Bidanel J P,Milan D,Iannuccelli N,et al.Detection of quantitative trait loci for growth and fatness in pigs[J].Genet Sel Evol,2001,33(3):289-309.
[10] Rattink A P,De Koning D J,Faivre M,et al.Fine mapping and imprinting analysis for fatness trait QTLs in pigs[J].Mamm Genome,2000,11:656-661.
[11] Meidtner K,Schwarzenbacher H,Scharfe M,et al.Haplotypes of the porcine peroxisome proliferator-activated receptor delta gene are associated witll backfat thickness[J].BMC Genet,2009,10:76.
[12] Gondret F,Riquet J,Tacher S,et al.Towards candidate genes affecting body fatness at the SSC7 QTL by expression analyses[J].J Anim Breed Genet,2012,129(4):316-324.
[13] Ren J,Duan Y,Qiao R,et al.A missense mutation in PPARD causes a major QTL effect on ear size in pigs[J].PLoS Genet,2011,7(5):e1002043.
[14] Duan Y,Brenig B,Wu X,et al.The G32E functional variant reduces activity of PPARD by nuclear export and post-translational modification in pigs[J].PLoS One,2013,8(9):e75925.
[15] Luquet S,Lopez-Soriano J,Holst D,et al.Roles of peroxisome proliferator-activated receptor delta (PPAR delta) in the control of fatty acid catabolism.A new target for the treatment of metabolic syndrome[J].Biochimie,2004,86(11):833-837.
[16] Grimaldi P A.Regulatory role of peroxisome proliferator-activated receptor delta (PPAR delta) in muscle metabolism.A new target for metabolic syndrome treatment[J].Biochimie,2005,87(1):5-8.
[17] Takada I,Yu R T,Xu H E,et al.Alteration of a single amino acid in peroxisome proliferator-activated receptor-alpha (PPAR alpha) generates a PPAR delta phenotype[J].Mol Endocrinol,2000,14(5):733-740.
[18] Hihi A K,Michalik L,Wahli W.PPARs:Transcriptional effectors of fatly acids and their derivatives[J].Cell Mol Life Sci,2002,59(5):790-798.
[19] Lee C H,Kang K,Mehl I R,et al.Peroxisome proliferator-activated receptor delta promotes very low-density lipoprotein-derived fatty acid catabolism in the macrophage[J].Proc Natl Acad Sci USA,2006,103(7):2434-2439.
[20] Wolf G.The function of the nuclear receptor peroxisome proliferator-activated receptor delta in energyhomeostasis[J]. Nutr Rev,2003,61(11):387-390.
[21] Fredenrich A,Grimaldi P A.Roles of peroxisome proliferator-activated receptor delta in skeletal muscle function and adaptation[J].Curr Opin Clin Nutr Metab Care,2004,7(4):377-381.
[22] Wang Y X,Lee C H,Tiep S,et al.Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity[J].Cell,2003,113:159-170.
[23] Muoio D M,MacLean P S,Lang D B,et al.Fatty acid homeostasis and induction of lipid regulatory genes in skeletal muscles of peroxisome proliferator-activated receptor (PPAR)alpha knock-out mice.Evidence for compensatory regulation by PPAR delta[J].J Biol Chem,2002,277:26089-26097.
[24] Tanaka M,Matsumoto T,Yanai S,et al.Conservation of the syntenies between porcine chromosome 7 and human chromosomes 6,14 and 15 demonstrated by radiation hybrid mapping and linkage analysis[J].Anita Genet,2003,34:255-263.
[25] 于晓静,苏本利,王晓梅,等. PPARδ-87T/C 基因多态性与糖代谢指标及肿瘤坏死因子浓度的研究[J].中国现代医学杂志,2009,19(22):3395-3397.
[26] Yu Y H,Wu S C,Cheng W T,et al.Ectopic expression of porcine peroxisome proliferator-activated receptor delta regulates adipogenesis in mouse myoblasts[J].J Anim Sci,2008,86:64-72.
[27] Dressel U,Allen T L,Pippal J B,et al.The peroxisome proliferator-activated receptor beta/delta agonist,GW501516,regulates the expression of genes involved in lipid catabolism and energy uncoupling in skeletal muscle cells[J].Mol Endocrinol,2003,17(12):2477-2493.
[28] Kim K S,Larsen N,Short T,et al.A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness,growth,and feed intake traits[J].Mamm Genome,2000,11:131-135.
[29] Liu G L,Jiang S W,Xiong Y Z,et al.Molecular screening of MC4R gene and association with fat traits in pig resource family[J].Acta Genetica Sinica,2002,29(6):497-501.

北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析

Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs

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