犬瘟热诊断技术的研究进展

doi:10.16431/j.cnki.1671-7236.2015.05.043

摘要/Abstract

摘要： 近年来,犬瘟热已严重危害了犬及毛皮动物养殖业的健康发展,快速、准确的诊断技术对于犬瘟热的预防显得尤为重要。现阶段犬瘟热诊断技术的研究主要有病毒分离培养与镜检观察、酶联免疫吸附试验(ELISA)、免疫组化、免疫胶体金、核酸杂交、巢式RT-PCR、实时荧光定量RT-PCR、双重PCR、基因芯片、液相芯片、环介导等温扩增等。获得能产生典型细胞病变的易感细胞株是成功进行犬瘟热病毒分离培养与镜检观察的关键。作者就犬瘟热诊断技术的研究进展作一综述,为有效预防和控制犬瘟热的流行和暴发提供简便、高效的检测技术。

关键词: 犬瘟热; 诊断技术; 研究进展

Abstract: In recent years, canine distemper is a serious damage to health development of dog and fur animal breeding industry.Fast and accurate diagnostic technique is particularly important for canine distemper prevention.At the present stage, canine distemper diagnostic techniques mainly include virus isolation, culture and microscopy observation, enzyme-linked immunosorbent assay(ELISA), immunohistochemistry, immune colloidal gold technique, nucleic acid hybridization technique, nested RT-PCR, Real-time fluorescence quantitative RT-PCR, double PCR, gene chip technology, liquid phase chip technology, loop-mediated isothermal amplification technology, and so on.The key of isolation, culture and microscopy observation of canine distemper virus is to obtain a susceptible cell lines that can produce typical CPE.We review research progress on diagnostic techniques of canine distemper, so as to provide simple and highly effective detecting technique for preventing and controlling epidemic and outbreak of canine distemper.

Key words: canine distemper; diagnostic technique; research progress

中图分类号:

S852.65

苏凤艳, 李哲, 刘千辉, 王全凯. 犬瘟热诊断技术的研究进展[J]. , 2015, 42(5): 1318-1324.

SU Feng-yan, LI Zhe, LIU Qian-hui, WANG Quan-kai. Research Progress on Diagnostic Technique of Canine Distemper[J]. , 2015, 42(5): 1318-1324.

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