关键词: 活性肽Temporin-Lb; 活性肽Catesbeianin-1a; 圆二色谱法(CD); MTS细胞毒试验; 体外抑瘤

Abstract: The study was aimed to research the growth inhibitory effects of two new bioactive peptides Temporin-Lb and Catesbeianin-1a from Rana catesbeiana on human lung cancer NCI-H446 cells, breast cancer MCF-7 cells and mice leukemia K562 cells, and provide the basis for selecting the new peptide antitumor drugs.The second structures of two bioactive peptides were tested by circular dichroism spectrum (CD), and the effects of the two new bioactive peptides on human lung cancer NCI-H446 cells, breast cancer MCF-7 cells and mice leukemia K562 cells were examined with MTS cytotoxicity assay.The circular dichroism spectrum results showed the secondary structure of Temporin-Lb was PPⅡ, and the secondary structure of Catesbeianin-1a was random coil.Using MTS cytotoxicity assay, it was found that given Catesbeianin-1a, the sharp of the three cancer cells above had little changed after culturing for 24 h, and the three cancer cells promoted normal.Given Temporin-Lb, the cell morphology of three cancer cells had changed after culturing for 24 h, the growth of the three cancer cells above had been inhibited, especially the bioactive peptide Temporin-Lb had a sharp antitumor effect to mice leukemia K562 cells between the concentration of 4 and 40 μmol/L.Bioactive peptide Catesbeianin-1a had no obvious effect on proliferation of the three cancer cells above.Bioactive peptide Temporin-Lb had a certain inhibitory effect to tumor cells.

Key words: bioactive peptide Temporin-Lb; bioactive peptide Catesbeianin-1a; circular dichroism spectrum (CD); MTS cytotoxicity assay; antitumor effect in vitro