生物钟基因在绒山羊皮肤中表达模式的比较

doi:10.16431/j.cnki.1671-7236.2015.02.002

›› 2015, Vol. 42 ›› Issue (2): 251-257.doi: 10.16431/j.cnki.1671-7236.2015.02.002

生物钟基因在绒山羊皮肤中表达模式的比较

贾丽丽^1,2, 金凤^1,2, 付绍印^1,2, 郑竹清^1,2, 杜琛^1,2, 季小阳^1,2, 王志新^1,2, 李金泉^1,2, 刘少卿⁴, 张文广^1,2,3

1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 内蒙古自治区动物育种与繁殖重点实验室, 呼和浩特 010018;
3. 内蒙古ATCG生物信息研究所, 呼和浩特 010020;
4. 内蒙古白绒山羊种羊场, 鄂托克旗 016100

收稿日期:2014-08-21 出版日期:2015-02-20 发布日期:2015-02-13
通讯作者: 张文广 E-mail:atcgnmbi@aliyun.com
作者简介:贾丽丽(1987-),女,内蒙古包头人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:zhihuijialili@163.com;金凤(1962-),女,蒙古族,内蒙古通辽人,硕士生,副教授,研究方向:动物遗传育种与繁殖,E-mail:jinfeng4961@163.com
基金资助:
国家自然科学基金(31260538、30960246);内蒙古自治区应用技术研究与开发资金计划(2013)

Comparation of Expression Pattern of Clock Genes in Cashmere Goat Skin

JIA Li-li^1,2, JIN Feng^1,2, FU Shao-yin^1,2, ZHENG Zhu-qing^1,2, DU Chen^1,2, JI Xiao-yang^1,2, WANG Zhi-xin^1,2, LI Jin-quan^1,2, LIU Shao-qing⁴, ZHANG Wen-guang^1,2,3

1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction of the Inner Mongol Autonomous Region, Hohhot 010018, China;
3. Institute of ATCG, Inner Mongol Bio-Information, Hohhot 010020, China;
4. Breeding Field of Inner Mongolia White Cashmere Goat, Etuokeqi 016100, China

Received:2014-08-21 Online:2015-02-20 Published:2015-02-13

摘要/Abstract

摘要： 文章旨在研究绒山羊皮肤生物钟基因clock、tim、per1和cry1的表达模式,进而分析其在绒山羊皮肤组织中的作用及相互关系。生物钟基因形成一个转录－翻译反馈环,通过钟基因在绒山羊皮肤生长周期进行高表达,激活per1和cry1基因的转录－转化过程,从而在mRNA和蛋白水平的调控下进行有节律的表达。结果表明,这些生物钟基因在绒山羊皮肤中的表达量依次为:clock >tim >per1 >cry1;皮肤次级毛囊兴盛期,高振幅clock、per1、tim、cry1的节律表达分别出现在一天中的04:00、08:00、08:00、16:00;皮肤次级毛囊休止期,这些基因的高振幅节律表达分别出现在一天中的08:00、04:00、04:00、16:00。另外,cry1、per1基因的表达在生物钟基因正反馈循环中被激活,tim基因的表达在生物钟基因的负反馈循环中被激活。因此,clock、tim、per1和cry1调控下运行的钟基因反馈环是绒毛生长呈周期性现象的基础。

关键词: 山羊; clock; tim; cry1; per1; 皮肤; 表达模式

Abstract: The purpose of this study was to research expression pattern of clock,tim,per1 and cry1 genes in cashmere goats skin,and to analysis the role of Clock genes as well as relationship of tissue development in cashmere goat skin.Clock genes form a transcription-translation feedback loop,clock gene expression was higher through the growth cycle of skin in cashmere goat,activating per1, cry1 genes transcription-conversion process,which regulated mRNA and protein levels of the core Clock genes with rhythmic expression.The result showed that the expression levels of Clock genes in cashmere goat's skin was:clock >tim >per1 >cry1.The period of anagen in skin hair follicle,rhythmic expression of high-amplitude clock,per1,tim,cry1 appeared at 04:00,08:00,08:00,16:00;The periods of telogen in skin hair follicle,rhythmic expression of high-amplitude clock,per1,tim,cry1 appeared at 08:00,04:00,04:00,16:00.Furthermore,the cry1 and per1 genes expression were activated in the Clock genes positive feedback loop,the tim gene expression was activated in a negative feedback loop.In short,under the regulation of clock,tim,per1 and cry1 feedback loop,which operated cyclical phenomenon of pile.

Key words: goat; clock; tim; cry1; per1; skin; expression pattern

中图分类号:

Q786

贾丽丽, 金凤, 付绍印, 郑竹清, 杜琛, 季小阳, 王志新, 李金泉, 刘少卿, 张文广. 生物钟基因在绒山羊皮肤中表达模式的比较[J]. , 2015, 42(2): 251-257.

JIA Li-li, JIN Feng, FU Shao-yin, ZHENG Zhu-qing, DU Chen, JI Xiao-yang, WANG Zhi-xin, LI Jin-quan, LIU Shao-qing, ZHANG Wen-guang. Comparation of Expression Pattern of Clock Genes in Cashmere Goat Skin[J]. , 2015, 42(2): 251-257.

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生物钟基因在绒山羊皮肤中表达模式的比较

Comparation of Expression Pattern of Clock Genes in Cashmere Goat Skin

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