鸡血藤总黄酮对大肠杆菌败血症的治疗作用

doi:10.16431/j.cnki.1671-7236.2019.05.034

Abstract

Abstract:

In order to evaluate the therapeutic effect of total flavonoids from Spatholobus suberectus Dunn (TFSD) on Escherichia coli (E.coli) sepsis,sepsis animal models were established in mice by intraperitoneally injected with E.coli,mice were administrated with various dose of TFSD after 1 h,the clinical manifestations of mice were observed and scored after 12 h.Then the mice were dissected,the liver,thymus and spleen were observed and weighted for the calculation of their organ indexes,pathological sections of livers were prepared for detail analysis of pathological change.Blood routine indicators were measured to analyze the levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in serum.The levels of inflammatory cytokines such as interleukin-1β (IL-1β),IL-2,IL-6,IL-10 and tumor necrosis factor α (TNF-α) in serum were determined.The results showed that the injection of E.coli resulted in irregular hair coat,poor spirit,inflamed eyes,increase of secretion from eyes,tachypnea,even convulsion or shock.Atrophy was observed from liver,thymus and spleen tissues,corresponding with reduced organ index.Pathological sections indicated disorder of hepatic cord,swell of hepatic sinus,fuzziness of cell edges,and infiltration of inflammatory cells.The concentrations of leukocytes (WBC),lymphocyte (LYM),hemoglobin (HGB),red blood cell (RBC) and platelet (PLT) were significantly altered by the injection of E.coli (P<0.05).The levels of ALT,AST,LDH and inflammatory cytokines were significantly increased (P<0.05).However,the treatment of TFSD and amikacin was able to alleviate the clinical manifestation and inhibit the change of organ indexes,which tended to be similar to the control group.The results indicated that TFSD was able to alleviate E.coli induced injury by improving the immune response and inhibit inflammatory reaction in mice.

Key words: total flavonoid of Spatholobus suberectus Dunn (TFSD); Escherichia coli; sepsis

CLC Number:

S853.74

HUANG Shiqi, WEI Yani, ZHAO Yuchuan, ZHANG Bo, WANG Yuntao, LUO Miao, LAN Hong, WEI Liuyuan, YIN Xiaoyan, SI Hongbin, CHEN Hailan. Therapeutic Effect of Total Flavonoid of Spatholobus suberectus Dunn on Escherichia coli Sepsis[J]. , 2019, 46(5): 1541-1550.

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Therapeutic Effect of Total Flavonoid of Spatholobus suberectus Dunn on Escherichia coli Sepsis

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