猪伪狂犬病病毒天津变异株R13139对猪的致病性研究

doi:10.16431/j.cnki.1671-7236.2018.09.035

Abstract

Abstract:

In order to determine the pathogenicity of the pseudorabies virus (PRV) variant R13139 strain in pigs,PRV R13139 strain and PRV classic virulent SC strain were inoculated intranasally with 10⁶ TCID₅₀ to inoculate each of 3 healthy piglets at 6 and 9 weeks of age,and blank control group pigs were intranasally with saline.The pathogenicity difference of the two strains were evaluated by clinical manifestations,gross pathology,histopathology,antibody production and virus distribution in organs of infected pigs.The results showed that the lethality rates of SC strain to 6 and 9 weeks old pigs were 66.7%(2/3) and 100%(3/3) respectively,while the lethality rates of R13139 strain to 6 and 9 weeks old pigs were 33.3%(1/3) and 66.7%(2/3) respectively,indicating that R13139 strain was lower mortality than SC strain to 6 and 9 weeks old pigs;However,compared to SC strain infection,R13139 strain caused an earlier onset of clinical signs,higher temperature rise in the same time period and more serious neurologic symptoms in 6 and 9 weeks old pigs;The gross pathology showed that the liver lesions caused by R13139 strain had more obvious necrosis than those of SC strain;The pathological changes of heart,lung,spleen,bladder and brain were not very different;Histopathological examination revealed that the pathological damage caused by R13139 strain of tonsil,lung,liver and trigeminal ganglia of infected pigs was remarkably obvious than those of SC strain;Meanwhile,the PCR results showed the R13139 strain was more widely distributed in tonsil,spleen,lung,trigeminal ganglion and lymph node (inguinal,mesenteric and submandibular) of dead pig's organ samples;The results of ELISA detection showed the R13139-infected pig's PRV-gB antibodies produced earlier for 1 d than those of the SC-infected pigs.The results could provide data for revealing the causes of the reepidemic of pseudorabies in China and further research on the prevention and control technology.

Key words: pseudorabies virus (PRV); variant strain; pathogenicity

CLC Number:

S852.65⁺1

REN Weike, ZHANG Li, LI Xiuli, CHI Jingjing, TIAN Xiangxue, LI Fuqiang, DONG Zhimin, YAN Minghua, QIU Huaji. Pathogenicity of Pseudorabies Virus Tianjin Variant Strain R13139 in Pigs[J]. , 2018, 45(9): 2620-2627.

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Pathogenicity of Pseudorabies Virus Tianjin Variant Strain R13139 in Pigs

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