重组人溶菌酶对黄羽肉鸡的安全性评价

doi:10.16431/j.cnki.1671-7236.2023.10.013

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (10): 3994-4004.doi: 10.16431/j.cnki.1671-7236.2023.10.013

重组人溶菌酶对黄羽肉鸡的安全性评价

刘淑扬^1,5, 张健玲³, 王志林², 邓俊劲², 李洋², 张华⁴, 黄金锐⁴, 连培荣⁴, 陈碧娴⁴, 郭秋霞⁴, 黄嘉麒⁴, 张浩吉¹, 李家洲^5,6

1. 佛山科学技术学院, 佛山 528000;
2. 广东省农业科学院农业生物基因研究中心, 广州 510640;
3. 江门海关技术中心, 江门 529030;
4. 广州奇龙生物科技有限公司, 广州 510663;
5. 广东省农业科学院动物科学研究所, 广州 510630;
6. 岭南现代农业科学与技术广东省实验室河源分中心, 河源 517000

收稿日期:2023-03-07 出版日期:2023-10-05 发布日期:2023-09-26
通讯作者: 张浩吉;李家洲 E-mail:zhanghaoji@aliyun.com;lijiazhou@gdaas.cn
作者简介:刘淑扬,E-mail:1834344230@qq.com
基金资助:
广东省基础与应用基础研究基金项目(区域联合基金-重点项目2021B1515120006)；广东省科技专项资金(“大专项+任务清单”)项目(江科【2021】183号)；“十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(2022SDZG09)

Safety Evaluation of Recombinant Human Lysozyme on Yellow Feather Broilers

LIU Shuyang^1,5, ZHANG Jianling³, WANG Zhilin², DENG Junjin², LI Yang², ZHANG Hua⁴, HUANG Jinrui⁴, LIAN Peirong⁴, CHEN Bixian⁴, GUO Qiuxia⁴, HUANG Jiaqi⁴, ZHANG Haoji¹, LI Jiazhou^5,6

1. Foshan University, Foshan 528000, China;
2. Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
3. Jiangmen Customs Technical Center, Jiangmen 529030, China;
4. Guangzhou Qilong Biotechnology Co., Ltd., Guangzhou 510663, China;
5. Institute of Animal Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510630, China;
6. Guangdong Laboratory for Lingnan Modern Agriculture Heyuan Sub-center, Heyuan 517000, China

Received:2023-03-07 Online:2023-10-05 Published:2023-09-26

摘要/Abstract

摘要： 【目的】研究饲粮中添加不同水平重组人溶菌酶对黄羽肉鸡生长性能、血液生理、血浆生化、组织结构变化和肠道菌群结构的影响,从而评价重组人溶菌酶在肉鸡上应用的生物安全性。【方法】试验采用单因子完全随机设计,选用192只1日龄健康快速型黄羽肉鸡,随机分为4组,每组48只(公母对半),分别设置推荐剂量组(20 000 U/kg)、3倍推荐剂量组(60 000 U/kg)、5倍推荐剂量组(100 000 U/kg),在饲粮中填加相应剂量的重组人溶菌酶;另设置一基础饲粮组作为对照组,试验期为54 d,称量黄羽肉鸡在21、42和54 d时的空腹重量,统计饲料消耗量,计算试验期肉鸡的平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G);试验结束时,每组选择8只黄羽肉鸡采集血液,以检测血液常规生理指标与血浆生化指标;屠宰后采集鸡心脏、肝脏、脾脏、肺脏、肾脏、空肠和回肠组织,进行组织病理学检查;采集鸡盲肠内容物,检测盲肠微生物菌群结构。【结果】靶动物安全性试验期间各剂量组的所有肉鸡临床表现均正常。与对照组相比,各试验组生长性能无显著差异(P＞0.05);在血液生理参数方面,除饲粮添加60 000 U/kg重组人溶菌酶组的红细胞分布宽度变异系数显著低于对照组(P＜0.05)外,其他血液生理指标各试验组均无显著变化(P＞0.05)。在血浆生化参数方面,添加60 000 U/kg重组人溶菌酶组血浆肌酐含量显著高于对照组(P＜0.05),其他指标各组之间无显著变化(P＞0.05)。对添加不同水平重组人溶菌酶肉鸡进行剖检和组织病理学检查,与对照组相比,均未发现明显差异。肠道微生物组结果显示,饲喂不同水平重组人溶菌酶对肉鸡肠道菌群的多样性和结构也无显著影响(P＞0.05)。【结论】在黄羽肉鸡饲粮中添加20 000 U/kg重组人溶菌酶,具有5倍以上安全系数,即饲粮中添加100 000 U/kg以下重组人溶菌酶对黄羽肉鸡是安全的。

关键词: 黄羽肉鸡; 重组人溶菌酶; 安全性评价

Abstract: 【Objective】 To study the effects of adding different levels of recombinant human lysozyme to the diet on growth performance,blood physiology,plasma biochemistry,tissue structure changes,and intestinal microbiota structure of Yellow feather broilers,in order to evaluate the biological safety of recombinant human lysozyme application in broilers.【Method】 The experiment was conducted using a single factor completely randomized design,with 192 1-day-old healthy fast Yellow feathered broilers randomly divided into 4 groups,with 48 chickens in each group (male and female in half).The recommended dose group (20 000 U/kg),3 times the recommended dose group (60 000 U/kg),and 5 times the recommended dose group (100 000 U/kg) were set up,and a basic diet group was also set as the control group.The experimental period was 54 days.The fasting weight of Yellow feathered broilers was measured at 21,42,and 54 days,and the feed consumption was counted.The average daily gain (ADG),average daily feed intake (ADFI),and feed to gain ratio (F/G) of the broilers during the experimental period were calculated.At the end of the experiment,8 Yellow feathered broilers were selected from each group to collect blood for testing of routine physiological and plasma biochemical indicators.Collect chicken heart,liver,spleen,lungs,kidneys,jejunum,and ileum tissues for histopathological examination after slaughter.Collect chicken cecal contents and detect the structure of cecal microbiota.【Result】 During the experimental period,all broilers in each dose group displayed normal clinical signs.There was no significant difference in growth performance between the test groups and the control group(P＞0.05).There was also no significant difference in blood physiological parameters(P＞0.05) except than the variation coefficient of red blood cell distribution width,which was considerably greater in 60 000 U/kg group (P＜0.05).The plasma albumin of 60 000 U/kg group was significantly lower than that of the control group (P＜0.05),while other plasma biochemical markers did not significantly differ between the groups (P＞0.05).When broilers fed with various quantities of recombinant human lysozyme were autopsied and histopathologically examined in comparison to the control,no discernible change was discovered.Feeding different levels of recombinant human lysozyme had no significant effect on the diversity and structure of intestinal microflora in broilers (P＞0.05).【Conclusion】 Adding 20 000 U/kg recombinant human lysozyme to the diet of Yellow feather broilers had a safety factor of more than 5 times,indicating that adding less than 100 000 U/kg recombinant human lysozyme to the diet was safe for Yellow feather broilers.

Key words: Yellow feather broiler; recombinant human lysozyme; safety evaluation

中图分类号:

S831.5

刘淑扬, 张健玲, 王志林, 邓俊劲, 李洋, 张华, 黄金锐, 连培荣, 陈碧娴, 郭秋霞, 黄嘉麒, 张浩吉, 李家洲. 重组人溶菌酶对黄羽肉鸡的安全性评价[J]. 中国畜牧兽医, 2023, 50(10): 3994-4004.

LIU Shuyang, ZHANG Jianling, WANG Zhilin, DENG Junjin, LI Yang, ZHANG Hua, HUANG Jinrui, LIAN Peirong, CHEN Bixian, GUO Qiuxia, HUANG Jiaqi, ZHANG Haoji, LI Jiazhou. Safety Evaluation of Recombinant Human Lysozyme on Yellow Feather Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 3994-4004.

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重组人溶菌酶对黄羽肉鸡的安全性评价

Safety Evaluation of Recombinant Human Lysozyme on Yellow Feather Broilers

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