云南3个新发掘地方鸡种mtDNA D-loop区序列遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2020.10.022

摘要/Abstract

摘要： 为研究在云南省怒江州及山区发现的3个地方鸡种翻毛鸡（FM）、赤脖鸡（CB）和YN鸡的群体遗传背景，试验采用线粒体DNA（mitochondral DNA，mtDNA）D-loop区序列为遗传标记，对3个鸡种共168个样品进行了检测分析。结果显示，翻毛鸡、赤脖鸡和YN鸡3个地方鸡种共定义了27种单倍型，其单倍型多样度分别为0.947、0.938和0.596；核苷酸多样度分别为0.01268、0.01434、0.00239。系统发生树分析显示，本研究检测的168个样品分布在A、B、C、E、F和G 6个世系中，其中翻毛鸡包含E、F和G 3个世系；赤脖鸡包含A、B、C、E、F和G 6个世系，E、F和G世系为其主要世系；YN鸡包含E、F和G 3个世系，其中E世系是其主要世系。YN鸡主要与印度亚种、白洛克鸡、白来航鸡和新汉夏鸡等蛋鸡品种进化关系较近，而赤脖鸡血缘较杂乱，与滇南亚种、海南亚种、指名亚种、印度尼西亚斗鸡及老挝鸡进化关系较近；翻毛鸡与赤脖鸡共享了较多的单倍型，2个品种间的进化关系较近。本试验结果为新发现的3个云南地方鸡品种的母系起源和遗传评估提供了依据。

关键词: 翻毛鸡; 赤脖鸡; YN鸡; mtDNA D-loop区; 遗传多样性

Abstract: This study was aimed to evaluate the information of their genetic background of Frizzle chicken (FM),Naked-neck chicken (CB) and YN chicken (YN) that were three newly discovered native chicken genetic resources with excellent characteristics,which had been found in Nujiang prefecture and mountainous area of Yunnan province.The variation in a total of 168 individuals sampled from the three chicken populations was assessed using the mitochondrial DNA (mtDNA) D-loop region sequences as genetic marker.The results showed that there were a total of 27 haplotypes were defined in the three native chicken as well as the haplotype diversity of these three chicken were 0.947,0.938 and 0.596,respectively.The nucleotide diversity of these three chicken were 0.01268,0.01434 and 0.00239,respectively.Phylogenetic tree displayed that all 168 individuals distributed in maternal lineage A,B,C,E,F and G.Frizzle chicken contained lineages E,F and G,and Naked-neck chicken contained all 6 lineages,of which the main lineages were E,F and G.YN chicken included lineages E,F and G,and lineage E was the highest percentage in this population.Also,it found that YN chicken was closely related to Gallus gallus murghi,White Plymouth Rock chicken,White Leghorn chicken and New Hampshires chicken,while Naked-neck chicken was closely related to Gallus gallus spadiceus,Gallus gallus jabouillei,Gallus gallus gallus Linnaeus,Indonesian cockfight and Laos chicken.The Frizzle chickens shared more haplotypes with Naked-neck chicken,and the evolutionary relationship between the two species was closer.This study provided a basis for the origin and genetic assessment of three newly discovered Yunnan local chicken breeds.

Key words: Frizzle chicken; Naked-neck chicken; YN chicken; mitochondrial DNA D-loop region; genetic diversity

中图分类号:

S813.3

王坤, 豆腾飞, 李琦华, 柳明正, 李丰耘, 贾俊静, 葛长荣. 云南3个新发掘地方鸡种mtDNA D-loop区序列遗传多样性分析[J]. 中国畜牧兽医, 2020, 47(10): 3232-3241.

WANG Kun, DOU Tengfei, LI Qihua, LIU Mingzheng, LI Fengyun, JIA Junjing, GE Changrong. Genetic Diversity Analysis of Three Newly Discovered Native Chicken Breeds in Yunnan Using mtDNA D-loop Region Sequences[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3232-3241.

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