体外产气法筛选基于真姬菇菌糟碱贮的山羊日粮配方

doi:10.16431/j.cnki.1671-7236.2019.07.010

摘要/Abstract

摘要：

试验选取6只6月龄、体重（25±2.5） kg、安装瘤胃瘘管的健康川中黑山羊为瘤胃液供体；采用ANKOM RFS体外产气系统筛选基于真姬菇菌糟复合碱贮的山羊全混合日粮（TMR）配方。试验日粮按NRC山羊营养需要量配制，真姬菇菌糟复合碱贮在其中的添加比例分别为0、20%、30%、40%（以DM计，分别记为CK、SMS2、SMS3、SMS4组），每个水平3次重复，测定发酵0、6、12、18、24、30、36、42、48 h的产气量（GP），并测定48 h发酵液中挥发性脂肪酸（VFA）、微生物蛋白（MCP）、氨态氮（NH₃-N）含量、pH和体外真消化率（IVTD）。结果表明：SMS2、SMS3、SMS4组的GP_{48 h}、IVTD、MCP、TVFA较对照组分别提高了6.87%（P<0.01）、9.40%（P<0.01）、4.22%（P<0.05）；10.98%（P<0.01）、16.11%（P<0.01）、18.17%（P<0.01）；4.01%（P<0.05）、8.59%（P<0.05）、10.08%（P<0.05）及12.96%（P<0.05）、24.93%（P<0.01）、31.09%（P<0.01）。各组发酵液pH为6.66~6.70，NH₃-N浓度为16.96~18.45 mg/100 mL，乙酸丙酸比（A/P）为3.68~4.33，均在适宜范围之内。综合试验结果，真姬菇菌糟复合碱贮可以代替部分粗饲料，在育肥山羊全混合日粮（TMR）中的适宜添加比例为30%。

关键词: 山羊; 菌糟; 碱贮; 体外产气; 配方筛选

Abstract:

In this experiment,6 healthy Chuanzhong Black goats ((25±2.5) kg) were selected and rumen fistula was installed for rumen fluid collection.The ANKOM RFS in vitro gas production system was used to select the diet formula based on compound alkali storage of spent mushroom substrate.The test formula was prepared according to the nutrient requirement of NRC goats.The addition ratio of the compound alkali storage of spent mushroom substrate was 0,20%,30%,40% (CK,SMS2,SMS3,SMS4),and each level was repeated 3 times.The gas production (GP) at 0,6,12,18,24,30,36,42 and 48 h was measured,and the volatile fatty acids (VFA),microbial protein (MCP),ammonia nitrogen (NH₃-N),pH and in vitro true digestibility (IVTD) in fermentation broth at 48 h were determined.The results showed that the GP_{48 h},IVTD,MCP and TVFA of the experimental groups SMS2,SMS3 and SMS4 compared with control group,were increased by 6.87%(P<0.01),9.40%(P<0.01),4.22%(P<0.05);10.98%(P<0.01),16.11%(P<0.01),18.17%(P<0.01);4.01%(P<0.05),8.59%(P<0.05),10.08%(P<0.05) and 12.96%(P<0.05),24.93%(P<0.01),31.09%(P<0.01),respectively.The pH of four groups ranged from 6.66 to 6.70,NH₃-N ranged from 16.96 to 18.45 mg/100 mL,and A/P ranged from 3.68 to 4.33,they were all within the appropriate range.In conclusion,compound alkali storage of spent mushroom substrate could replace part of the roughage,and the recommended dosage was 30% for the fattening goat diet.

Key words: goat; spent mushroom substrate; alkali storage; gas production in vitro; formula screening

中图分类号:

S816.8

许锦聪, 潘双子, 苗景, 何祥波, 梁学武, 甘乾福. 体外产气法筛选基于真姬菇菌糟碱贮的山羊日粮配方[J]. 《中国畜牧兽医》, 2019, 46(7): 1962-1968.

XU Jincong, PAN Shuangzi, MIAO Jing, HE Xiangbo, LIANG Xuewu, GAN Qianfu. Goat Formula Screening Based on Spent Mushroom (Hypsizygus marmoreus) Substrate Alkali Storage by In vitro Gas Production[J]. , 2019, 46(7): 1962-1968.

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