›› 2012, Vol. 39 ›› Issue (2): 203-206.

• 流行病防治 • Previous Articles     Next Articles

Investigation of Drug and Disinfectants Resistance of Pig Source E.coli O157∶H7

LI Jun1, XIE Yu-zhou1, FENG Shi-wen2, PENG Hao1, CHEN Ze-xiang1, XUAN Xiong-biao1, HU Shuai1, MA Chun-xia1, XIE Yong-ping1, YANG Wei1, ZENG Yun2   

  1. 1. Guangxi Veterinary Research Institute,Nanning 530001,China;2. College of Animal Science and Technology,Guangxi University,Nanning 530005,China
  • Received:1900-01-01 Revised:2011-08-02 Online:2012-02-20 Published:2012-02-20

Abstract: To investigate the information of drug resistance phenotypes and disinfectants resistance of pig source E.coli O157∶H7 isolated from Guangxi,the drug sensitive and disinfectants resistance were assayed. Meanwhile,3 aminoglycoside antibiotic resistance genes,aph(3)-Iia,aadA and aadB were amplified by PCR. The results of drug sensitive to 27 antibiotics showed that 5 strains were sensitive to Florfenicol,Ceftriaxone,Cefoxitin and Cefotaxime. The rate of drug resistance to Roxithromycin,Polymyxin B,Rifampin,Lincomycin,Amoxycilin,Ampicilin and Cephalothin were 100%. The rate of drug resistance to other antibiotics,such as Trobicin,Streptomycin,Cefradine were between 20% and 60%. Among of 5 strains,1 strain,1 strain and 3 strains were resistances to 23,11 and 9 antibiotics,respectively. The results of PCR confirmed that genome of 5 strains contained aminoglycoside antibiotic resistance genes. Antibiotic resistance genes had a certain correlation with drug resistance phenotypes. The results of disinfectants resistance showed that 5 strains were resistance to Povidone lodine solution,Bromogeramine solution,Glutaraldehyde solution,double quaternary ammonium salt-iodine disinfection liquid and compound of peracetic acid,except dischloroisocyanuric acid sodium chloride powder. Our results will provide information to prevention and control of E.coli O157∶H7 in Guangxi.

Key words: E.coli O157∶H7; drug resistance; disinfectant resistance

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