Abstract: In order to improve the resistance of probiotics on the adverse environmental, and to make them reach the stomach by active state and play the corresponding effect, use bacteria that our laboratory isolated as experimental objects, and use the technology of microcapsules coated with a combination of freeze-drying, design L9 (34) orthogonal test, to filter the best formula of preparation of microencapsulated Lactobacillus,and detection the characteristics of Lactobacillus microcapsules. The test results proved that with the sodium alginate concentration of 3%, calcium chloride concentration of 2%, skim milk powder concentration of 4%, lactose concentration of 6%, the embedding yields of viable bacteria in microcapsules reached 92.40%, and the product has good acid resistance and intestinal-lysis,and it has good storage stability at room temperature.

Key words: Lactobacillus from chicken; microcapsules; freeze drying; orthogonal test