芝麻素对氟暴露斑马鱼生长性能及肠组织形态学的影响

doi:10.16431/j.cnki.1671-7236.2019.08.018

Abstract

Abstract:

In order to study the effects of sesamin on the growth performance and the morphology of intestinal tissues of zebrafish, 720 zebrafish were randomly divided into 6 groups:Control group (CK), sesamin Ⅰ group (1 g, S1), sasamin Ⅱ group (2 g, S2), 80 mg/L fluoride group (F), 80 mg/L fluoride+sesamin Ⅰ group (1 g, FS1), and 80 mg/L fluoride+sasamin Ⅱ group (2 g, FS2), with 3 replicates per group and 40 zebrafish per replicate.The body length, body weight, LGR, WGR, and SGR were determined and the intestine structures of zebrafish were observed using the histological method, and the height of intestinal villi were measured using the Imageproplus at 45th and 90th day.The results showed that at 45 d, compared with the control group, the body weight of zebrafish in group F was significantly decreased (P<0.05), and sesamin treatment could alleviate the decrease of body weight, however the difference was not significant (P>0.05).At 90 d, the body weight and body length of zebrafish in group F were remarkably reduced compared with the control group (P<0.05), and sesamin treatment could relieve the negative effects caused by fluoride to some extent, but the difference was not significant (P>0.05).After exposure for 45 and 90 d, the LGR, WGR, and SGR of zebrafish in group F were decreased compared to the control group, while they showed an increasing trend in groups FS1 and FS2 compared with the group F.Sesamin treatment could alleviate the damage caused by fluoride in intestinal tissue of zebrafish according to the tissue section of gut.All above results indicated that sesamin could attenuate the toxic effects of fluoride on growth performance and the intestinal tissues in zebrafish.

Key words: fluoride; sesamin; zebrafish; growth performance; intestinal tissue

CLC Number:

S917

GUO Wenjing, WANG Tianyu, WANG Guodong, WU Panhong, HE Xinjin, CHEN Jianjie, CAO Jinling, LUO Yongju. Effects of Sesamin on Growth Performance and Intestinal Tissue of Zebrafish Exposed to Fluoride[J]. , 2019, 46(8): 2338-2345.

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Effects of Sesamin on Growth Performance and Intestinal Tissue of Zebrafish Exposed to Fluoride

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