吉林某牛场牛病毒性腹泻流行病学调查及分析

doi:10.16431/j.cnki.1671-7236.2019.01.029

Abstract

Abstract:

In order to totally understand the prevalence of bovine viral diarrhea (BVD) in a large-scale cattle farm in Jilin province,a total of 157 clinical serum samples,18 stool samples,17 liver and semen tissue samples were collected.Serum antibody detection was performed using BVDV antibody detection kit.BVDV antigen detection was performed on serum and clinical samples by Nano-PCR method and BVDV type 1 specific primers,the antigen positive results were sequenced and analyzed;The antigen positive samples were ground and diluted,filtered and sterilized by 0.22 μm filter membrane,and connected to MDBK cells for virus isolation and culture.The antigen detection was carried out again after three generations;The infection of MDBK cells was detected by immunofluorescence technique;Phylogenetic tree and homology analysis were drawn by Mega software.The results showed that the positive rate of clinical serum BVDV antibody in the cattle farm was 77.1%,the positive rate of serum antigen was 12.1%,and the positive rate of sample antigen in clinical feces was 74.3%.After the diseased material was connected to the cells,the cytopathic effect could not be observed.PCR results of the obtained strains were consistent with the results of sample antigen detection.The immunofluorescence assay results showed that BVDV venom was normally adsorbed in MDBK cells,and there was obvious fluorescence reaction.The main strain of BVD and BVDV JL-1 strain were analyzed by antigen sequencing,the homology was as high as 99.0%,and both were BVDV type 1 strains.This study had conducted a comprehensive survey of the prevalence of BVDV in the cattle farm,laying the foundation for the purification work.

Key words: bovine viral diarrhea (BVD); epidemiological investigation; virus isolation

CLC Number:

S855.3

LIU Zeyu, LI Jianyou, LIU Zhankui, LI Zhijie, WANG Chao, GUO Li. Epidemiological Investigation and Analysis of Bovine Viral Diarrhea of One Cattle Farm in Jilin Province[J]. , 2019, 46(1): 247-255.

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Epidemiological Investigation and Analysis of Bovine Viral Diarrhea of One Cattle Farm in Jilin Province

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